Ultrasound diagnosis of structural abnormalities in the first trimester

The advances in ultrasound technology have made it possible to identify fetal structural abnormalities and genetic syndromes in the first trimester. First trimester prenatal diagnosis of fetal central nervous system, renal, gastrointestinal, cardiac, and skeletal abnormalities is reviewed. Copyright © 2002 John Wiley & Sons, Ltd.     

A Multi‐Scale Soil Moisture Monitoring Strategy for California: Design and Validation

A multi‐scale soil moisture monitoring strategy for California was designed to inform water resource management. The proposed workflow classifies soil moisture response units (SMRUs) using publicly available datasets that represent soil, vegetation, climate, and hydrology variables, which control soil water storage. The SMRUs were classified, using principal component analysis and unsupervised K‐means clustering within a geographic information system, and validated, using summary statistics derived from measured soil moisture time series. Validation stations, located in the Sierra Nevada, include transect of sites that cross the rain‐to‐snow transition and a cluster of sites located at similar elevations in a snow‐dominated watershed. The SMRUs capture unique responses to varying climate conditions characterized by statistical measures of central tendency, dispersion, and extremes. A topographic position index and landform classification is the final step in the workflow to guide the optimal placement of soil moisture sensors at the local‐scale. The proposed workflow is highly flexible and can be implemented over a range of spatial scales and input datasets can be customized. Our approach captures a range of soil moisture responses to climate across California and can be used to design and optimize soil moisture monitoring strategies to support runoff forecasts for water supply management or to assess landscape conditions for forest and rangeland management.     

The Route to Best Science in Implementation of the Endangered Species Act’s Consultation Mandate: The Benefits of Structured Effects Analysis

The Endangered Species Act is intended to conserve at-risk species and the ecosystems upon which they depend, and it is premised on the notion that if the wildlife agencies that are charged with implementing the statute use the best available scientific information, they can successfully carry out this intention. We assess effects analysis as a tool for using best science to guide agency decisions under the Act. After introducing effects analysis, we propose a framework that facilitates identification and use of the best available information in the development of agency determinations. The framework includes three essential steps—the collection of reliable scientific information, the critical assessment and synthesis of available data and analyses derived from those data, and the analysis of the effects of actions on listed species and their habitats. We warn of likely obstacles to rigorous, structured effect analyses and describe the extent to which independent scientific review may assist in overcoming these obstacles. We conclude by describing eight essential elements that are required for a successful effects analysis.     

Genetic structure of the southeastern United States loggerhead turtle nesting aggregation: evidence of additional structure within the peninsular Florida recovery unit

The southeastern United States supports one of two large loggerhead turtle (Caretta caretta) nesting aggregations worldwide and is therefore critical to global conservation and recovery efforts for the species. Previous studies have established the presence of four demographically distinct nesting populations (management units) corresponding to beaches from (1) North Carolina through northeastern Florida, (2) peninsular Florida, (3) the Dry Tortugas, and (4) northwest Florida. Temporal and geographic genetic structure of the nesting aggregation was examined utilizing partial mitochondrial control region haplotype frequencies from 834 samples collected over the 2002 through 2008 nesting seasons from 19 beaches as well as previously published haplotype data. Most rookeries did not exhibit interannual genetic variation. However, the interannual variation detected did significantly impact the interpretation of spatial genetic structure in northeastern Florida. Based on pairwise F _ST comparisons, exact tests of population differentiation, and analysis of molecular variance, the present study upholds the distinctiveness of the four currently recognized management units and further supports recognition of discrete central eastern, southern (southeastern and southwestern), and central western Florida management units. Further subdivision may be warranted, but more intensive genetic sampling is required. In addition, tools such as telemetry and mark-recapture are needed to complement genetic data and overcome limitations of genetic markers in resolving loggerhead turtle rookery connectivity in the southeastern USA.     

A Strategy for Prioritizing Threats and Recovery Actions for At-Risk Species

Ensuring the persistence of at-risk species depends on implementing conservation actions that ameliorate threats. We developed and implemented a method to quantify the relative importance of threats and to prioritize recovery actions based on their potential to affect risk to Mojave desert tortoises (Gopherus agassizii). We used assessments of threat importance and elasticities of demographic rates from population matrix models to estimate the relative contributions of threats to overall increase in risk to the population. We found that urbanization, human access, military operations, disease, and illegal use of off highway vehicles are the most serious threats to the desert tortoise range-wide. These results suggest that, overall, recovery actions that decrease habitat loss, predation, and crushing will be most effective for recovery; specifically, we found that habitat restoration, topic-specific environmental education, and land acquisition are most likely to result in the greatest decrease in risk to the desert tortoise across its range. In addition, we have developed an application that manages the conceptual model and all supporting information and calculates threat severity and potential effectiveness of recovery actions. Our analytical approach provides an objective process for quantifying threats, prioritizing recovery actions, and developing monitoring metrics for those actions for adaptive management of any at-risk species.     

Low or absent unconjugated estriol in pregnancy: an indicator for steroid sulfatase deficiency detectable by fluorescence in situ hybridization and biochemical analysis

It has been previously reported that a low or absent maternal serum unconjugated estriol (uE3) level is associated with placental steroid sulfatase (STS) deficiency. Here we report a correlation between patients who present with a very low or absent maternal serum uE3 and a deletion of the STS gene as assessed by fluorescence in situ hybridization (FISH). We studied nine prenatal cases that presented to the clinical laboratory with an abnormal triple screen, specifically low or absent maternal serum uE3 and a 46,XY karyotype. FISH analysis showed complete deletion of a probe containing the STS gene in six cases and one case had a partial deletion (reduced but not absent signal). The remaining two cases were not deleted for the STS probe. All mothers tested whose fetus showed a deletion were shown to be STS deletion carriers using FISH. Biochemical analysis was performed on 7/9 prenatal specimens. All fetuses deleted for the STS probe were also found to be deficient for STS by biochemical analysis of cultured amniotic fluid (5/5). Of the two fetuses not deleted for the STS probe, one was deficient for STS activity, while the other had a normal result. The abnormal result of enzyme deficiency by biochemical analysis in a non-deletion case likely represents a mutation in the STS gene, not detectable by this FISH assay. Postnatal FISH confirmation of the STS deletion was performed in 1/7 cases. Clinical follow-up was available for 4/9 cases following birth. Copyright © 2002 John Wiley & Sons, Ltd.