Biodegradability and Mechanical Properties of Poly(vinyl alcohol)-Based Blend Plastics Prepared Through Extrusion Method

Plastic blend materials consisting of poly(vinyl alcohol), glycerol and xanthan or gellan were prepared through laboratory extrusion. Their base mechanical properties were compared with the properties of poly(vinyl alcohol) foil and their biodegradability in soil, compost and both activated and anaerobic sludge were assessed. In samples with lower polysaccharide content (10–21 %w/w) the tensile strength of 15–20 MPa was found; the elongation at break of all blends was relatively close to the parameter of poly(vinyl alcohol) foil. The biodegradability levels of the blends tested corresponded to the content of natural components, and the mineralization of the samples with the highest carbohydrate proportion (42 %) reached 50–78 %, depending on the type of the environment. Complete biodegradation of all samples occurred in activated sludge.     

Seasonal changes in daily precipitation extremes in mainland Portugal from 1941 to 2007

This study aims mostly at understanding seasonal variations in the intensity, frequency and duration of extreme precipitation events in mainland Portugal. For this purpose, selected precipitation indices that mainly focus on extremes were calculated at the seasonal scale for daily data recorded in the period 1941–2007 at 57 meteorological stations scattered across the area. These indices were explored for trends at the local and regional levels. The results show that there are marked changes in precipitation indices at the seasonal scale. Trends in spring and autumn precipitation have opposite signals. In spring, statistically significant drying trends are found together with a reduction in extremes. In autumn, wetting trends are detected for all indices, although overall they are not significant at the 5 % level. In addition, the relationship between seasonal extreme precipitation indices and atmospheric large-scale modes of low-frequency variability is analysed by means of a seasonal correlation analysis. Four modes of low-frequency variability are explored. Results confirm that, over mainland Portugal, the North Atlantic Oscillation is one of the most important teleconnection patterns in any season and the mode of variability that has the greatest influence on precipitation extremes in the area, particularly in the winter and autumn.     

Sorption and desorption of sulfentrazone in Brazilian soils

This study was undertaken to obtain information about the behavior of sulfentrazone in soil by evaluating the sorption and desorption of the herbicide in different Brazilian soils. Batch equilibrium method was used and the samples were analyzed by high performance liquid chromatography. Based on the results obtained from the values of Freundlich constants (Kf), we determined the order of sorption (Haplic Planosol < Red-Yellow Latosol < Red Argisol < Humic Cambisol < Regolitic Neosol) and desorption (Regolitic Neosol < Red Argisol < Humic Cambisol < Haplic Planosol < Red-Yellow Latosol) of sulfentrazone in the soils. The process of pesticide sorption in soils was dependent on the levels of organic matter and clay, while desorption was influenced by the organic matter content and soil pH. Thus, the use of sulfentrazone in soils with low clay content and organic matter (low sorption) increases the probability of contaminating future crops.     

Effects of a constructional intervention on airborne and deposited particulate matter in the Portuguese National Tile Museum, Lisbon

In the 1970s, a large ambulatory of the National Tile Museum, Lisbon, was closed with glass panes on both ground and first floor. Although this design was meant to protect the museum collection from ambient air pollutants, small openings between the glass panes remain, creating a semi-enclosed corridor. The effects of the glass panes on the indoor air quality were evaluated in a comparative study by monitoring the airborne particle concentration and the extent of particle deposition at the enclosed corridor as well as inside the museum building. Comparison of the indoor/outdoor ratio of airborne particle concentration demonstrated a high natural ventilation rate in the enclosed corridor as well as inside the museum building. PM₁₀ deposition velocities on vertical surfaces were estimated in the order of 3?×?10^?4 m s^?1 for both indoor locations. Also, the deposition rates of dark-coloured and black particles in specific were very similar at both indoor locations, causing visual degradation. The effectiveness of the glass panes in protecting the museum collection is discussed.     

Variation patterns in individual fish responses to chemical stress among estuaries, seasons and genders: the case of the European flounder (Platichthys flesus) in the Bay of Biscay

The objective was to describe and model variation patterns in individual fish responses to contaminants among estuaries, season and gender. Two hundred twenty-seven adult European flounders were collected in two seasons (winter and summer) in four estuaries along the Bay of Biscay (South West France), focusing on a pristine system (the Ster), vs. three estuaries displaying contrasted levels of contaminants (the Vilaine, Loire and Gironde). Twenty-three variables were measured by fish, considering the load of contaminants (liver metals, liver and muscle persistent organic pollutants, muscle polycyclic aromatic hydrocarbons); the gene expression (Cyt C oxydase, ATPase, BHMT, Cyt P450 1A1, ferritin); the blood genotoxicity (Comet test); and liver histology (foci of cellular alteration–tumour, steatosis, inflammation, abnormal glycogen storage). Canonical redundancy analysis (RDA) was used to model these variables using gender, season and estuary of origin as explanatory variables. The results underlined the homogeneity of fish responses within the pristine site (Ster) and more important seasonal variability within the three contaminated systems. The complete model RDA was significant and explained 35 % of total variance. Estuary and season respectively explained 30 and 5 % of the total independent variation components, whilst gender was not a significant factor. The first axis of the RDA explains nearly 27 % of the total variance and mostly represents a gradient of contamination. The links between the load of contaminants, the expression of several genes and the biomarkers were analysed considering different levels of chemical stress and a possible multi-stress, particularly in the Vilaine estuary.     

Occurrence and levels of organochlorine compounds in human breast milk in Bangladesh

In low-income countries, the use of some organochlorine pesticides is still common in order to increase food production. Monitoring the chemical exposure is an important step in risk-reducing strategies. This is the first study to report concentrations of organochlorines in breast milk of women from Bangladesh where farming is the main income source.Organochlorines such as p,p′-DDT, o,p′-DDT, p,p′-DDE, p,p′-DDD (i.e., ∑DDT), HCB, α-, β- and γ-HCH, trans-chlordane, cis-chlordane, oxy-chlordane, trans-nonachlor, cis-nonachlor, mirex and polychlorinated biphenyls (CB 28, 52, 99, 101, 105, 114, 118, 123, 128, 138, 141, 149, 153, 156, 157, 163, 167, 170, 180, 183, 187, 189, 194) were analyzed in breast milk collected in 2002 from 72 first-time mothers (median age 20 years) living in the rural area Matlab, Bangladesh.While the concentrations of PCBs and many of the pesticides were low, the concentrations of p,p′-DDT and its metabolite p,p′-DDE were high (median 349 and 1645 ng g⁻¹ lipid, respectively) in comparison to other countries. The median value of ∑DDT was 2123 ng g⁻¹ lipid. The estimated daily exposure to p,p′-DDT, p,p′-DDE and ∑DDTs was 10, 30 and 42 μg kg⁻¹ body weight, respectively, in 3 months old infants. The p,p′-DDE/p,p′-DDT ratio ranged from 1 to 23, where 58% of the mothers had a ratio below 5 indicating recent or ongoing DDT exposure.This study reports infant exposure and maternal body burden of organochlorines through breast milk. Although the findings give no reason to limit breast-feeding, it is essential to identify the main exposure sources and find means to decrease the exposure.     

Purification of carbonic anhydrase from bovine erythrocytes and its application in the enzymic capture of carbon dioxide

This work presents a study of industrially applicable techniques to obtain a biologically supported carbon dioxide capture system, based on the extraction of carbonic anhydrase from bovine blood. Carbonic anhydrase is a metalloenzyme which catalyzes the reversible hydration of carbon dioxide. The objective of this study was to establish conditions to obtain carbonic anhydrase from bovine erythrocytes and apply it in the capture of carbon dioxide. To achieve this, two different purification techniques were evaluated: one by extraction with the organic solvents chloroform and ethanol, where different solvent proportions were studied; and the other by ammonium sulfate precipitation, testing percent saturations between 10% and 80%. Carbon dioxide was enzymatically captured by its precipitation as calcium carbonate with the enzyme obtained by both techniques. The enzyme extracted by ethanol and chloroform showed an activity of 2623 U mL⁻¹, recovery of 98% and purification factor of 104-fold. That precipitated by ammonium sulfate showed an activity of 2162 U mL⁻¹, recovery of 66% and purification factor of 1.4-fold using 60% ammonium sulfate saturation. The results obtained in the carbon dioxide capture experiments showed that the carbonic anhydrase extracted in this study not only enhanced the hydration of CO₂, but also promoted the formation of CaCO₃.     

Recycling of wastes from fish beneficiation by composting: chemical characteristics of the compost and efficiency of their humic acids in stimulating the growth of lettuce

Waste from the beneficiation of fish was composted with crushed grass aiming to characterize their chemical composition and investigate the possibility of the use of the final compost as source of humic acids (HA) able to stimulate the growth of lettuce. Compost presented pH value, C/N ratio, and electrical conductivity that allow its use as an organic fertilizer. The element content was present in the following order of abundance in the compost: P?>?Ca?>?N?>?Mg?>?K?>?Fe?>?Zn?>?Mn?>?Mo?>?Cu, and the humus composition was similar to that observed in others kind of organic residues composted. The high content of oxygen pointed out a high level of oxidation of HA, in line with the predominance of phenolic acidity in the functional groups. The ¹³C-NMR spectra showed marked resonances due to the presence of lipids and other materials resistant to degradation as methoxy substituent and N-alkyl groups. A concentration of 20 mg L^?1 HA increased significantly both dry and wet root matter in lettuce but the CO₂ assimilation, stomatal conductance, and number of lateral roots of the plants were not affected. However, increases of 64% in the water-use efficiency was observed due to the HA addition, probably related to the root morphology alteration which resulted in 1.6-fold increase of lateral root average length and due to the higher H⁺ extrusion activity. Reuse of residues from the fish beneficiation activity by composting may represent a safe tool to increase the value of recycled organic residues and generate HA with potential use as plant growth stimulants.     

Water quality and restoration in a coastal subdivision stormwater pond

Stormwater ponds are commonly used in residential and commercial areas to control flooding. The accumulation of urban contaminants in stormwater ponds can lead to a number of water quality problems including high nutrient, chemical contaminant, and bacterial levels. This study examined the interaction between land use and coastal pond water quality in a South Carolina residential subdivision pond. Eutrophic levels of chlorophyll and phosphorus were present in all seasons. Harmful cyanobacterial blooms were prevalent during the summer months. Microcystin toxin and fecal coliform bacteria levels were measured that exceeded health and safety standards. Low concentrations of herbicides (atrazine and 2,4-D) were also detected during summer months. Drainage from the stormwater pond may transport contaminants into the adjacent tidal creek and estuary. A survey of residents within the pond's watershed indicated poor pet waste management and frequent use of fertilizers and pesticides as possible contamination sources. Educational and outreach activities were provided to community members to create an awareness of the water quality conditions in the pond. Pond management strategies were then recommended, and selected mitigation actions were implemented. Water quality problems identified in this study have been observed in other coastal stormwater ponds of varying size and salinity, leading this project to serve as a potential model for coastal stormwater pond management.     

Decision support model for assessing aquifer pollution hazard and prioritizing groundwater resources management in the wet Pampa plain, Argentina

This paper gives an account of the implementation of a decision support system for assessing aquifer pollution hazard and prioritizing subwatersheds for groundwater resources management in the southeastern Pampa plain of Argentina. The use of this system is demonstrated with an example from Dulce Stream Basin (1,000 km² encompassing 27 subwatersheds), which has high level of agricultural activities and extensive available data regarding aquifer geology. In the logic model, aquifer pollution hazard is assessed as a function of two primary topics: groundwater and soil conditions. This logic model shows the state of each evaluated landscape with respect to aquifer pollution hazard based mainly on the parameters of the DRASTIC and GOD models. The decision model allows prioritizing subwatersheds for groundwater resources management according to three main criteria including farming activities, agrochemical application, and irrigation use. Stakeholder participation, through interviews, in combination with expert judgment was used to select and weight each criterion. The resulting subwatershed priority map, by combining the logic and decision models, allowed identifying five subwatersheds in the upper and middle basin as the main aquifer protection areas. The results reasonably fit the natural conditions of the basin, identifying those subwatersheds with shallow water depth, loam–loam silt texture soil media and pasture land cover in the middle basin, and others with intensive agricultural activity, coinciding with the natural recharge area to the aquifer system. Major difficulties and some recommendations of applying this methodology in real-world situations are discussed.