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31.
Euglena gracilis as a model for the study of Cu2+ and Zn2+ toxicity and accumulation in eukaryotic cells 总被引:1,自引:0,他引:1
Einicker-Lamas M Mezian GA Fernandes TB Silva FL Guerra F Miranda K Attias M Oliveira MM 《Environmental pollution (Barking, Essex : 1987)》2002,120(3):779-786
We have observed the effect of copper and zinc on the biology of Euglena gracilis. The cells displayed different sensitivities to these metals, as the apparent LC50 for Cu2+ was 0.22 mM, and for Zn2+ it was 0.88 mM. While Zn2+ was able to increase cell proliferation even at 0.1 mM, the minimal CuCl2 concentration tested (0.02 mM) was sufficient to impair cell division. Higher concentrations of these metals not only inhibited cell division in a concentration-dependent manner, but also interfered with the metabolism of E. gracilis. A higher accumulation of proteins and lipids per cell was observed at the DI50 concentration for metal-treated cells. These results suggest that the test concentration of both metals leads to a failure in completing cell division. Ultrastructural analysis indicated a chloroplast disorganization in copper-treated cells, as well as the presence of electron dense granules with different shapes and sizes inside vacuoles. Microanalysis of these granules indicated an accumulation of copper, thus suggesting a detoxification role played by the vacuoles. These results indicate that E. gracilis is an efficient biological model for the study of metal poisoning in eukaryotic cells. They also indicate that copper and zinc (copper being more poisonous) had an overall toxic effect on E. gracilis and that part of the effect can be ascribed to defects in the structure of chloroplast membranes. 相似文献
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Godoi Marcelo Leitemberger Andrielli Böhs Lucas Martins Correa Silveira Marcia Victoria Rafique Jamal D’Oca Marcelo Gonçalves Montes 《Environmental Chemistry Letters》2019,17(3):1441-1446
Environmental Chemistry Letters - Herein, we describe a simple and inexpensive protocol for the hydrothiolation of alkynes. In this context, water extract of rice straw ash (WERSA) has been proven... 相似文献
34.
Marcia Thais Suriano Alaide A. Fonseca-Gessner Fabio O. Roque Claudio G. Froehlich 《Environmental monitoring and assessment》2011,175(1-4):87-101
The development of biomonitoring programs based on the macroinvertebrate community requires the understanding of species distribution patterns, as well as of the responses of the community to anthropogenic stressors. In this study, 49 metrics were tested as potential means of assessing the condition of 29 first- and second-order streams located in areas of differing types of land use in São Paulo State, Brazil. Of the sampled streams, 15 were in well-preserved regions in the Atlantic Forest, 5 were among sugarcane cultivations, 5 were in areas of pasture, and 4 were among eucalyptus plantations. The metrics were assessed against the following criteria: (1) predictable response to the impact of human activity; (2) highest taxonomic resolution, and (3) operational and theoretical simplicity. We found that 18 metrics were correlated with the environmental and spatial predictors used, and seven of these satisfied the selection criteria and are thus candidates for inclusion in a multimetric system to assess low-order streams in São Paulo State. These metrics are family richness; Ephemeroptera, Plecoptera and Trichoptera (EPT) richness; proportion of Megaloptera and Hirudinea; proportion of EPT; Shannon diversity index for genus; and adapted Biological Monitoring Work Party biotic index. 相似文献
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Fernandes Rafaella F. Alves Gustavo A. S. Gonçalves Renato V. Temperini Marcia L. A. 《Journal of Polymers and the Environment》2021,29(10):3105-3115
Journal of Polymers and the Environment - Polymer fractions from a biological matrix have been well-characterized by vibrational spectroscopy, XPS, CP/MAS 13C NMR, MALDI-TOF mass... 相似文献
37.
Lory JA Massey RE Zulovich JM Hoehne JA Schmidt AM Carlson MS Fulhage CD 《Journal of environmental quality》2004,33(3):1106-1113
Water quality concerns and revised regulations are changing how confined animal feeding operations manage manure. Devising acceptable and feasible changes in manure practices requires a full understanding of the forces shaping current manure management decisions. Previous theoretical models have shown that a wide range of factors influence the lowest cost solution for manure management. We used a mechanistic model to characterize the manure management practices on 39 swine operations (20 unagitated lagoon and 19 slurry operations) in five states (Iowa, Missouri, North Carolina, Oklahoma, and Pennsylvania). Information was collected from each operation about animal numbers, feed and water use, manure handling and storage characteristics, field locations, crop rotation, fertilizer need, and equipment inventory and usage. Collected data were used as input and to validate results from a mechanistic model that determined acres required for manure application, manure application rate, time required for manure application, value of manure, and costs of manure management. The 39 farms had a mean of 984 animal units (AU) per operation, 18.2 AU ha(-1) (7.4 AU acre(-1)), and manure application costs of dollar 10.49 AU(-1) yr(-1). Significant factors affecting manure management included operation size, manure handling system, state, and ownership structure. Larger operations had lower manure management costs (r2 = 0.32). Manure value potentially exceeded manure application costs on 58% of slurry and 15% of lagoon operations. But 38% of slurry operations needed to apply manure off the farm whereas all lagoon operations had sufficient land for N-based manure management. Manure management was a higher percentage of gross income on contract operations compared with independents (P < 0.01). This research emphasized the importance of site-specific factors affecting manure management decisions and the economics of U.S. swine operations. 相似文献
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K Ebner D W Brewster F Matsumura 《Journal of environmental science and health. Part. B》1988,23(5):427-438
Serum insulin and glucose were measured in young male rabbits after a single intraperitoneal dose of 1 or 50 micrograms/kg of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Serum insulin levels in the high dosed rabbits were significantly decreased between 15 min and 8 h post treatment, equivalent after 24 h, significantly elevated 48 h post treatment, and they were not different at 10 days post-treatment when compared to weight matched and pair-fed controls. At the low dose, rabbits showed no differences in serum insulin from controls. In the high dose group, serum glucose levels were generally not different between treated and control animals, though there was a transient hyperglycemia 1 h after treatment, and both treated groups became hypoglycemic after ten days. The results indicate that TCDD altered serum insulin levels which were not coupled to changes in serum glucose. 相似文献
40.
Waha Katharina Krummenauer Linda Adams Sophie Aich Valentin Baarsch Florent Coumou Dim Fader Marianela Hoff Holger Jobbins Guy Marcus Rachel Mengel Matthias Otto Ilona M. Perrette Mahé Rocha Marcia Robinson Alexander Schleussner Carl-Friedrich 《Regional Environmental Change》2017,17(6):1623-1638
Regional Environmental Change - The Middle East and North Africa (MENA) region emerges as one of the hot spots for worsening extreme heat, drought and aridity conditions under climate change. A... 相似文献