Developing green infrastructure ‘thinking’: devising and applying an interactive group-based methodology for practitioners

Recent years have witnessed a wave of interest in the concept of green infrastructure (GI) as a means of applying an ecosystem approach to spatial planning practice; however, more limited attention has been paid to decision-making processes or tools to enhance GI within spatial plans and guidance. We address this deficit by reporting on the development and application of an interactive group-based methodology to enhance GI ‘thinking’ and interdisciplinary collaboration, drawing on the literature on the sociology of interactions. Our findings suggest that a game-based approach to GI problem-solving was successful in breaking down professional barriers by creating an informal learning arena, providing an enabling opportunity for participants to solve problems in an iterative, non-linear style to develop principles for action with transferability to ongoing plan formation. This style of problem-solving was characterised by shifting norms and routines of interaction, leading to problem re-framing and a search for alternative solutions.     

Rapid and Sensitive LC/MS/MS Direct Injection Method for the Determination of Trace Level Corexit EC9500A Oil Dispersant in Seawater

A rapid and sensitive liquid chromatography tandem mass spectrometry (LC/MS/MS) method for the determination of trace dioctyl sulfosuccinate (DOSS) concentrations in seawater samples has been established. The method is well suited to aquatic environment impact monitoring following application of the dispersant Corexit EC9500A. Linearity of the method was demonstrated down to 0.05 ng/mL^?1 (0.05 µgL^?1) DOSS in seawater, with a 2.4% relative standard deviation precision for preparation replicates. A US EPA method limit of detection of <0.02 ng/mL^?1 (<0.02 µgL^?1) was calculated and specificity was confirmed by monitoring of two qualifier ions at 291.1 m/z and 227.1 m/z. These transitions were confirmed by QToF analysis to be associated with the DOSS precursor ion at 421.2 m/z. For application to seawater samples and samples containing oil particulates, a practical and repeatable calibration range of 0.5 ng/mL^?1 (0.5 µgL^?1) to 25.0 ng/mL^?1 (25.0 µgL^?1) DOSS is reported. The method was shown to have excellent precision and accuracy, with a consistent ≤1.6% relative standard deviation for system suitability standards at 0.5 ng/mL^?1 (0.5 µgL^?1) and linear weighted (1/x) regression coefficients of determination ≥0.995. The surfactant nature of the analyte is discussed in relation to detection limit and loss of analyte. Speculation of a relationship between DOSS in association or aggregation with divalent cations, such as Ca²⁺ present in salt water and hard water, is suggested. The consequent effects on cell ionic balance and membrane function are discussed.     

The effect of oil sands process-affected water and naphthenic acids on the germination and development of Arabidopsis

Oil sands mining in the Athabasca region of northern Alberta results in the production of large volumes of oil sands process-affected water (OSPW). We have evaluated the effects of OSPW, the acid extractable organic (AEO) fraction of OSPW, and individual naphthenic acids (NAs) on the germination and development of the model plant, Arabidopsis thaliana (Arabidopsis). The surrogate NAs that were selected for this study were petroleum NAs that have been used in previous toxicology studies and may not represent OSPW NAs. A tricyclic diamondoid NA that was recently identified as a component of OSPW served as a model NA in this study. Germination of Arabidopsis seeds was not inhibited when grown on medium containing up to 75% OSPW or by 50 mg L⁻¹ AEO. However, simultaneous exposure to three simple, single-ringed surrogate NAs or a double-ringed surrogate NA had an inhibitory effect on germination at a concentration of 10 mg L⁻¹, whereas inhibition of germination by the diamondoid model NA was observed only at 50 mg L⁻¹. Seedling root growth was impaired by treatment with low concentrations of OSPW, and exposure to higher concentrations of OSPW resulted in increased growth inhibition of roots and primary leaves, and caused bleaching of cotyledons. Treatment with single- or double-ringed surrogate NAs at 10 mg L⁻¹ severely impaired seedling growth. AEO or diamondoid NA treatment was less toxic, but resulted in severely impaired growth at 50 mg L⁻¹. At low NA concentrations there was occasionally a stimulatory effect on root and shoot growth, possibly owing to the broad structural similarity of some NAs to known plant growth regulators such as auxins. This report provides a foundation for future studies aimed at using Arabidopsis as a biosensor for toxicity and to identify genes with possible roles in NA phytoremediation.     

Perceptions and attitudes towards gas flaring in the Niger Delta,Nigeria

Gas flaring is the burning of crude oil’s associated gas. In Nigeria’s Niger Delta, the flaring process is usually very close to communities and their farmlands and has been implicated in serious environmental degradation of the region. Studies have suggested links between gas flaring and health problems in the communities and others have established relationships between gas flaring and poor agricultural yields. This study explores how the people in the region perceive gas flaring and what their attitudes are toward it. Ubeji town, a community where gas flaring takes place, was selected as a case study. It was found that the residents perceive gas flaring as hazardous to health, environment, and general well-being of the community. Most residents seem to be resigned to the continued presence of gas flaring activities in the community. The study, however, raised several questions on modeling perception and attitudes toward environmental problems in areas where political tension and economic adversity are prevalent.     

Offshore windmills and the effects of electromagnetic fields on fish

With the large scale developments of offshore windpower the number of underwater electric cables is increasing with various technologies applied. A wind farm is associated with different types of cables used for intraturbine, array-to-transformer, and transformer-to-shore transmissions. As the electric currents in submarine cables induce electromagnetic fields there is a concern of how they may influence fishes. Studies have shown that there are fish species that are magneto-sensitive using geomagnetic field information for the purpose of orientation. This implies that if the geomagnetic field is locally altered it could influence spatial patterns in fish. There are also physiological aspects to consider, especially for species that are less inclined to move as the exposure could be persistent in a particular area. Even though studies have shown that magnetic fields could affect fish, there is at present limited evidence that fish are influenced by the electromagnetic fields that underwater cables from windmills generate. Studies on European eel in the Baltic Sea have indicated some minor effects. In this article we give an overview on the type of submarine cables that are used for electric transmissions in the sea. We also describe the character of the magnetic fields they induce. The effects of magnetic fields on fish are reviewed and how this may relate to the cables used for offshore wind power is discussed.     

Isolation and characterization of two actins of the Pacific white shrimp, <Emphasis Type="Italic">Litopenaeus vannamei</Emphasis>

Two actin genes named actinT1 and actinT2 were isolated and sequenced from the Pacific white shrimp, Litopenaeus vannamei, by screening from a shrimp eyestalk cDNA library. L. vannamei actinT1 cDNA has a 1,128-bp open reading frame encoding for 376 amino acids while L. vannamei actinT2 cDNA has a 1,131-bp open reading frame coding for 377 amino acids. Alignment of the actinT1 and actinT2 cDNA sequences showed that these two actin genes share a sequence identity of 86% at amino acid residues. When compared with actins of several other invertebrate and vertebrate species, the nucleotide sequence of actinT1 is highly homologous (97–100%) with beta-actins, while actinT2 shares 86–95% identity with alpha-actins on the nucleotide level. Phylogenetic analysis and BLAST searches indicated that the ActinT1 protein is identical to crustacean beta-actins, while the ActinT2 protein is highly homologous to crustacean alpha-actins. Constitutive expression of the actinT1 and actinT2 genes were detected by RT-PCR in all adult shrimp organs, including brain, eye-stalk, gill, heart, hemolymph, hepatopancreas, muscle, swimming legs, and stomach, as well as in the shrimp zygote, nauplius, and mysis life stages. These data will facilitate attempts to clone and identify more shrimp genes and constitutive shrimp promoters.     

Assigning results of the Tool for Sustainability Impact Assessment (ToSIA) to products of a forest-wood-chain

A Tool for Sustainability Impact Assessment (ToSIA) has been developed for assessing sustainability impacts of forest-wood-chains (FWCs). Sustainability is determined by analysing environmental, economic, and social sustainability indicators for all the production processes along the FWC. Results of the tool can be analysed at an aggregated level for complete FWCs, but for some applications it is useful to assign the indicator results to products of the chain.This paper presents a procedure in ToSIA to assign sustainability impacts to multiple output products of FWC. The procedure was tested and demonstrated with an example FWC from Scandinavia that included furniture and bio-energy production. Two different allocation criteria, carbon-based and economic value-based, were applied with different options for assigning the impacts on the sub-products of the chain. Three indicators representing the three pillars of the sustainability were chosen to demonstrate the procedure: production costs (economic), employment (social) and transport intensity (environmental).The results indicated that the allocation criteria greatly affect the indicator results assigned to the different products of FWCs. The selection of the allocation criterion depends on the question approached and on the availability of the needed process related data. The data availability is assured for the carbon-based allocation within ToSIA, as following the carbon flows within the chain is mandatory for any ToSIA application. Economic values of products, on the other hand, are more closely linked to the aims of the production processes of the value chains and are thereby meaningful allocation criteria in many cases. The allocation procedure of ToSIA was proved to be flexible allowing different criteria and still consistent in allocation of the various sustainability impacts of the FWCs.