Exploring the abundance and influencing factors of antimicrobial resistance genes in manure plasmidome from swine farms

Plasmids play a critical role in the dissemination of antimicrobial resistance genes (ARGs), however, a systematical understanding of ARGs originated from plasmids in swine production is currently lacking. Herein, quantitative polymerase chain reaction was applied to determine the prevalence of ten ARGs and the class1 integron gene intI1 of plasmid source in swine manure from 44 farms in Sichuan, Hubei and Hebei provinces, China. All assayed ARGs were observed in plasmid DNA samples, and the average absolute abundance of aac(6’)-Ib-cr, bla_NDM, bla_CTX-M, optrA, ermB, floR, mcr-1, qnrS, tetM, sul1 and intI1 were 7.09, 2.90, 4.67, 6.62, 7.55, 7.14, 4.08, 4.85, 7.16, 7.11 and 8.07 of 10 log copies/gram, respectively. IntI1 showed a high correlation (r > 0.8, P < 0.01) with the abundance of aac(6’)-Ib-cr and sul1 in swine manure. Moreover, the farm scale (i.e., herd population) and geographical location were not found to be critical factors influencing the absolute abundance of ARGs of plasmid DNA in swine farms. However, the concentrations of florfenicol, Cu, Zn, Fe, total phosphorus (TP) and total potassium (TK) demonstrated a significant correlation with the abundance of several ARGs. Particularly, Cu and Zn had high correlations with optrA and bla_CTX-M, respectively. Our results demonstrated that antibiotics, heavy metals and environmental nutrients are likely jointly contributing to the long-term persistence of ARGs in swine production. This study provides insights into the abundance and influencing factors of ARGs from swine manure, which is of significance for assessing and reducing the public health risks in livestock production.     

Electrically enhanced photodegradation of an azodye(Acid Orange Ⅱ) using a Pt/TiO2 film electrode irradiating with an UV lamp

A photoelectrochemical process in the degradation of an azodye(Acid Orange II)on a Pt/TiO2 film electrode was investigated.By using the glass device and the voltage stabilized source of direct current,decolorization ratios higher than 78% were observed during a period of 5h.Comparing this value with the sum of the decolorization ratios obtained by a sole application of electrochemical(lower than 3%)and photochemical(about 23%)procedures, a significant synergic effect between both processes was observed.The effects of adscititious voltage and pH value on the decolorization ratios were obvious while the effect of the amount of aeration was minor.     

民国初年灾荒史研究综述

自民国迄今,一些政治家、思想家及学者分别从自然科学角度和社会历史角度对民国初年灾荒史予以诠释,依时间而言主要集中于20世纪80年代以后,依内容而言又呈现出一种多元化的态势。其间,虽然取得了一定的成就,诸如积累了一些资料、研究内容多样化等,但仍存在着一些不足。     

Spatial and temporal distribution of illegal dumping sites in the nature protected area: the Ojców National Park,Poland

The existence of illegal dumping continues to be a worldwide problem, even in nature protected areas, and its distribution is not random. An understanding of the distribution of illegal dumping sites is crucial for the enhancement of effective waste management systems. Therefore, this study aims at a better understanding of spatial and temporal changes to illegal dumping sites in a nature protected area (the Ojców National Park) from 1994 to 2016. The most important spatial factors that control the distribution of illegal dumping sites were the distance from roads and from the field-forest edge. In the last two decades, the number of small dumping sites has increased, whereas the number of large illegal dumping sites has decreased. Moreover, this study presents a model of the potential occurrence of illegal dumping sites, which indicates places that should be under the control of the national park and of local authorities.     

Emissions of intermediate volatility organic compound from waste cooking oil biodiesel and marine gas oil on a ship auxiliary engine

Ship auxiliary engines contribute large amounts of air pollutants when at berth.Biodiesel,including that from waste cooking oil(WCO),can favor a reduction in the emission of primary pollutant when used with internal combustion engines.This study investigated the emissions of gaseous intermediate-volatile organic compounds(IVOCs) between WCO biodiesel and marine gas oil(MGO) to further understand the differences in secondary organic aerosol(SOA) production of exhausts.Results revealed that WCO exhaust exhibited similar IVOC composition and volatility distribution to MGO exhaust,despite the differences between fuel contents.While WCO biodiesel could reduce IVOC emissions by 50% as compared to MGO,and thus reduced the SOA production from IVOCs.The compositions and volatility distributions of exhaust IVOCs varied to those of their fuels,implying that fuel-component-based SOA predicting model should be used with more cautions when assessing SOA production of WCO and MGO exhausts.WCO biodiesel is a cleaner fuel comparing to conventional MGO on ship auxiliary engines with regard to the reductions in gaseous IVOC emissions and corresponding SOA productions.Although the tests were conducted on test bench,the results could be considered as representative due to the widely applications of the test engine and MGO fuel on real-world ships.     

Observation of chemical components of PM2.5 and secondary inorganic aerosol formation during haze and sandy haze days in Zhengzhou, China

Mineral dust particles play an important role in the formation of secondary inorganic aerosols, which largely contribute to haze pollution in China.During this study, a haze episode(haze days) and a typical haze process mixed with sandstorm(sandy haze days)were observed in Zhengzhou with a series of high-time-resolution monitoring instruments from November 22 to December 8, 2018.Concentrations of PM_(10) and crustal elements clearly increased in the sandy haze days.Concentrations of gaseous pollutants, metallic elements emitted from anthropogenic sources, nitrate, and ammonium during sandy haze days were slightly lower than those during the haze days but still obviously higher than those during the non-haze days.The sulfate concentrations, the sulfate fractions in PM_(2.5),and the sulfur oxidation ratios significantly increased in the sandy haze days.Heterogeneous reactions dominated the conversion of SO_2 during the haze and sandy haze days.Enhanced SO_2 conversion during the sandy haze days may be attributed to the high concentrations of transition metal ions from the sandstorm when the values of relative humidity(RH) were in 30%–70%, and high O_3 at certain time points.Gas-phase NO_2 oxidation reactions were the main pathways for nitrate formation.In the sandy haze days,higher nitrogen oxidation ratio(NOR) at daytime may be associated with higher RH and lower temperature than those in the haze days, which facilitate the gas-to-particle partitioning of nitrate; higher NOR values at night may be attributed to the higher O_3 concentrations, which promoted the formation of N_2O_5.     

Spatiotemporal characterization and regional contributions of O3 and NO2: An investigation of two years of monitoring data in Henan, China

To investigate the characteristics of ground level ozone(O_3) for Henan Province,the ambient air quality monitoring data of 2015 and 2016 were analyzed.The result showed that the 8 h-max-O_3 concentrations displayed a distinct seasonality,where the maximum and minimum values,averaging 140.41,54.19 μg/m~3,occurred in summer and winter,respectively.There is a significant correlation between meteorological factors and O_3 concentration.The Voronoi neighborhood averaging analysis indic ated that O_3,temperature,and ultraviolet radiation in Henan province were decreased from northwest to southeast,while relative humidity and precipitation displayed the opposite trend.Besides meteorological factors,the chemical processes play an essential role in ozone formation.Reactions of NO,NO_2 and O_3 form a closed system,and the partitioning point of the OX-component(O_3+NO_2) was at 40 and 80 μg/m~3 for nitrogen oxide(NO_x) in winter and summer,respectively,with NO_2 dominating at higher NO_x pollution and O_3 being the m ajor component at lower levels.The relationship between oxidant(OX=O_3+NO_2)and NOx concentrations were evaluated to understand the regional and local contribution of OX.It showed that high regional contribution occurred in the spring,whereas the highest local contribution lead to the summer peak of O_3 observed in Zhengzhou.This present study reveals important environment impacts from the photochemical process and the meteorological conditions in the region with better understanding on the O_3 characterization.     

氧化木糖无色杆菌(Achromobacte rxylosoxidans)LAX2对Cu、Pb和Cd复合污染土壤的生物矿化修复研究

我国土壤重金属复合污染较为突出,是目前亟待解决的土壤环境问题之一.本文研究了一株氧化木糖无色杆菌LAX2对Cu、Pb和Cd共存体系的生物矿化作用及其复合污染土壤的矿化修复作用.结果表明,菌株LAX2的发酵液、无菌发酵液和菌体细胞对3种重金属的去除能力大小顺序均为Pb~(2+)Cd~(2+)Cu~(2+).X-射线衍射、扫描电镜、红外光谱和能谱分析表明,3种重金属共存时菌株LAX2发酵液可诱导形成PbCO_3和CdCO_3晶体,而Cu不能单独成矿,混合矿物晶体呈长杆状.菌株LAX2发酵液能够明显降低黑钙土和白浆土中Cu、Pb和Cd的有效态含量,矿化修复30 d后,黑钙土中Cu、Pb、Cd的有效态含量分别降低了48.0%、71.4%、62.8%,白浆土中Cu、Pb、Cd的有效态含量分别降低了42.0%、63.2%、53.6%;碳酸盐结合态和铁锰氧化物结合态含量明显增加,可交换态和有机物结合态含量明显降低.矿化修复后的土壤中重金属的浸出毒性随修复时间的增长而降低,黑钙土中Cu、Pb、Cd浸出量分别降低了90.3%、93.2%、92.8%,白浆土中Cu、Pb、Cd浸出量分别降低了82.5%、86.1%、84.3%.以上结果说明,菌株LAX2可通过碳酸盐矿化作用固定土壤中的复合重金属,且在相同条件下对黑钙土的修复效果好于白浆土.