Prenatal exclusion of homocystinuria (cystathionine β-synthase deficiency) by assay of phytohaemagglutinin-stimulated fetal lymphocytes

Homocystinuria due to cystathionine β-synthase deficiency was excluded in a fetus at 23 weeks' gestation by demonstrating activity of the enzyme in fetal lymphocytes after stimulation by phytohaemagglutinin. Fetal blood sampling was carried out because two determinations of enzyme activity on cultured amniotic cells gave low, not fully diagnostic values.     

NITRATE CONCENTRATIONS IN GROUNDWATER BENEATH A BEEF CATTLE FEEDLOT1

ABSTRACT. A study of nitrate concentration in the groundwater beneath a beef cattle feedlot near Central City, Nebraska was started in 1968. An intensive 3-day pumping study was conducted at the feedlot at the start of 1970 irrigation system. Little nitrate concentration was found in the ground-water coming from beneath the feedlot. Pumping caused a slight increase in nitrate concentration over the average concentration for the previous 2 years.     

Methodological aspects of a national population-based study of persistent organochlorine compounds in serum

Key methodological aspects are presented for a study of concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), polychlorinated biphenyls (PCBs), and organochlorine pesticides in the serum of a sample of the New Zealand population aged 15 years and older. The study took advantage of the sampling frame and sample collection and interview processes of the National Nutrition Study (NNS). An additional blood sample for this organochlorines study was collected by the NNS and questions added to the NNS questionnaire. Serum was obtained from the blood and, based on responses to questions in the questionnaire, samples with possible occupational exposure to organochlorines were excluded. Remaining samples providing at least 2 ml of serum were pooled within 80 strata defined according to geographic area, age group, sex and ethnicity. A minimum number of five individual serum samples was required for pooling within a stratum. Within strata with sufficient samples, two or three pooled samples were created for variance calculation. Eligible for inclusion in the study were 2497 individual serum samples. Sixty strata had sufficient serum samples for pooling and chemical analysis. This was the first study of organochlorine compounds with a national population-based sample. Two factors that made the study feasible deserve emphasis. First, being able to "piggy-back" on another study. Second, pooling of samples to reduce analytic expenses. It is hoped that the methods used in this study will form the basis for other studies investigating organochlorine concentrations in national populations.     

The recovery of selenious acid aerosols on glass fiber filters

Previous workers have shown that selenium is only partially trapped on a filter during air sampling. In some cases, these losses have been attributed to volatilization of selenium dioxide. Our results demonstrate that selenium dioxide, in the presence of moist air, is completely recovered (apparently as selenious acid aerosols) and that the previous shortfalls must be due to other selenium species as yet unidentified. Selenious acid aerosols in our study were formed by volatilizing selenium dioxide (approximately 3 mg) into a stream of moist ambient air (relative humidity, greater than 50%), and trapped on glass fiber filters using a high-volume air sampler. Selenium(IV) was ultrasonically extracted from the filter with water and analyzed by atomic absorption spectrometry. Selenious acid aerosols were trapped on the filters with high efficiency (105 +/- 5 percent) using a 50 minute sampling period. With an extended sampling period (24 hours) the recovery was 103 +/- 6 percent.     

Future directions in air-quality science,policy, and education

On February 12-15, 2001, more than 200 scientists, engineers, decision makers, and educators participated in a conference on the "Future Directions in Air Quality Research: Ecological, Atmospheric, Regulatory/Policy, and Educational Issues." Important perspectives are summarized from the keynote addresses of noted scientists and educators, as well as managers in government, industry, and public interest groups. Observations and recommendations are provided to stimulate further thought about how to increase opportunities to make greater use of scientific knowledge in air-quality decision making and to ensure that decisions are effective, economically viable, health and ecologically sound, and socially acceptable. Recommendations are given regarding ways in which communications between scientists and policy makers should be structured so as to make appropriate and effective use of scientists and the knowledge they can provide in policy-making fora.     

Bacterial sources,pathways and management strategies for urban runoff

The microbiological quality of diffuse impermeable surface runoff is described in terms of bacterial densities and pathogens observed within urban catchments in North London and Milton Keynes and the use of somatic bacteriophages as faecal indicators are evaluated. The studies show the occurrence of faecal indicator organisms (FIOs) and pathogens to be ubiquitous in stormwater runoff from all types of urban land use surfaces, with the possible exception of major highways. Urban catchments in North London show a progressive downstream increase in FIOs and pathogens consonant with increasing urbanization and incidence of stormwater outfalls and combined sewer overflows (CSOs). Surface water FIOs and pathogens appear to be predominantly of non‐human origin being primarily derived from animal and bird sources, although the effect is over‐ridden in the presence of misconnections and CSO discharges. A combination of infrastructure improvement, end‐of‐pipe detention, source control and more robust local authority regulation is recommended for effective management and remediation of bacteriological urban water quality.     

CONSIDERATIONS OF SCALE IN WATER QUALITY MONITORING AND DATA ANALYSIS1

ABSTRACT: An assumption of scale is inherent in any environmental monitoring exercise. The temporal or spatial scale of interest defines the statistical model which would be most appropriate for a given system and thus affects both sampling design and data analysis. Two monitoring objectives which are strongly tied to scale are the estimation of average conditions and the evaluation of trends. For both of these objectives, the time or spatial scale of interest strongly influences whether a given set of observations should be regarded as independent or serially correlated and affects the importance of serial correlation in choosing statistical methods. In particular serial correlation has a much different effect on the estimation of long-term means than it does on the estimation of specific-period means. For estimating trends, a distinction between serial correlation and trend is scale dependent. An explicit consideration of scale in monitoring system design and data analysis is, therefore, most important for producing meaningful statistical information.     

A comparison of traditional,cluster and Zürich-Montpellier analyses of infaunal pelecypod associations from two adjacent sediment beds

A Zürich-Montpellier analysis provided more detailed information about pelecypod associations in two sediment beds than either a traditional subjective approach or a cluster analysis based on Jaccard's coefficient. The Zürich-Montpellier test separated till and sand pelecypod associations, and indicated distinguishing species and atypical samples. Species distinguishing the till association were Cardita ventricosa, Yoldia myalis, Semele rubropicta, Venerupis kennerlyi, Clinocardium nuttallii, Nuculana minuta and Macoma incongrua. Species distinguishing the sand were Macoma elimata, Compsomyax subdiaphana, Yoldia ensifera, Macoma calcarea, M. lipara, M. brota, Yoldia limatula and Macoma alaskana. Two samples contained species from both associations plus some species rare elsewhere. Combined R-and Q-type grouping procedures, of which the Zürich-Montpellier approach is an example, are useful in evaluating benthic faunal similarities, but the suitability of various forms of the procedure needs further study.Supported by the Defense Research Board of Canada, Grant No. 9520-23. Based on a thesis submitted by J. D. Popham to the University of Victoria in partial fulfillment of the requirements for the degree of B.Sc. (Honours).     

Quantitative and qualitative observations on Laminaria dentigera and other subtidal kelps of southern Kodiak Island,Alaska

Observations were made and quantitative samples of non-canopy kelps were collected, using SCUBA, at 9 subtidal sites off southern Kodiak Island in May, 1976. At a 10th site, only observations were made. The most abundant species in the quadrats were Laminaria dentigera, L. yezoensis, Pleurophycus gardneri, Agarum cribrosum, and Alaria marginata. We found fertile plants of all these species. L. dentigera dominated at all sampled sites except one within a bed of Nereocystis luetkeana, where P. gardneri was dominant. L. yezoensis was dominant at the site which was visited but not sampled. The average wet weight of non-canopy kelps in the 55 samples was 12 kg m^-2, and the quadrat range was 2 to 35 kg m^-2. The reported ranges of P. gardneri and Alaria marginata are extended westward from Montague Island (147°22W) to Bumble Bay, Kodiak Island (154°43W), where A. marginata was found at a depth of 8.5 m.     

Evaluation of a gradient sampling design for environmental impact assessment

Before-After Control Impact (BACI) sampling designsare commonly used in environmental impact assessmentand are considered the most effective for detectingchanges due to anthropogenic disturbances. Thesedesigns handle local spatial variability throughrandomized placement of samples into a treatmentstratum and a control stratum. When a contaminantdisperses with distance from a point source it issuggested that a gradient design will be moresensitive to change than randomized placement ofsamples. This requires allocating samples according todistance, rather than by random placement withinrandomly placed blocks. In this paper gradient versusrandom sampling designs were compared using data froman oil field in the North Sea. The gradient samplingdesign was more powerful than a randomized CI sampling design.