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961.
We describe two applications of a recently introduced system for very precise, continuous measurement of water oxygen saturation. Oxygen microoptodes (based on the dynamic fluorescence quenching principle) with a tip diameter of ~50 µm, an eight-channel optode array, an intermittent flow system, and online data registration were used to perform two types of experiments. The metabolic activity of Antarctic invertebrates (sponges and scallops) was estimated in respiration experiments, and, secondly, oxygen saturation inside living sponge tissue was determined in different flow regimes. Even in long-term experiments (several days) no drift was detectable in between calibrations. Data obtained were in excellent correspondence with control measurements performed with a modified Winkler method. Antarctic invertebrates in our study showed low oxygen consumption rates, ranging from 0.03-0.19 cm3 O2 h-1 ind.-1. Oxygen saturation inside living sponge specimens was affected by flow regime and culturing conditions of sponges. Our results suggest that oxygen optodes are a reliable tool for oxygen measurements beyond the methodological limits of traditional methods. 相似文献
962.
Laurie B. Connell Scott P. MacQuarrie Betty M. Twarog Melissa Iszard V. Monica Bricelj 《Marine Biology》2007,150(6):1227-1236
The softshell clam, Mya arenaria, is a commercially important bivalve with wide latitudinal distribution in North America. Populations of clams with a history
of repeated exposure to toxic Alexandrium spp. have developed a natural resistance to the paralytic shellfish toxins (PSTs) produced by these algae. An association
between PST resistance in individual clams and a single mutation in the saxitoxin (STX) binding region of the α-subunit of
the voltage-gated sodium (Na+) channel gene was previously identified. Here we establish that more than one mutation associated with nerve resistance to
STX occurred at this locus. Both cDNA from mRNA and genomic DNA sequences from individual clams are identical demonstrating
that both alleles are expressed simultaneously. In addition, one resistant allele per individual is sufficient to confer neural
resistance to STX even though heterozygous individuals show an intermediate level of resistance to STX in in vitro nerve trunk
assays. 相似文献
963.
Abstract: Populations of Marianas fruit bats, Pteropus mariannus, were surveyed on each of the 15 Mariana Islands in 1983–1984. It is estimated that a minimum of 8,700–9,000 fruit bats occur in the archipelago, with about 8245% of these bats found on the nine northernmost and largely uninhabited islands. The islands of Anatahan, Pagan, and Agrihan had the largest populations, with minimum population estimates of 3,000, 2,500, and 1,000 bats, respectively. Smaller populations of about 400–1,000 fruit bats occurred on Asuncion, Guam, Rota, and Guam. The remaining is-ands in the archipelago were each estimated to have fewer than 125 bats. Population densities of fruit bats were highest on islands with little bunting but were generally much lower on human-inhabited islands where bunting was common. Quantity and quality of existing habitat were other important factors regulating the size of fruit bat populations in the Marianas. Increased enforcement of existing laws protecting bats and a public awareness program are important tasks needed to conserve and manage fmit bats in the Marianu Islands. The census techniques used in this study may be applicable to other fypes of colonial, mobile wildlife that inhabit islands. 相似文献
964.
Summary Home ranges, areas of intensive use, and locations of nest sites were determined for female Columbian ground squirrels and their daughters over seven years from a low-density population in southwestern Alberta. Adult females usually retained similar home ranges from year to year. However, they moved their nest sites more often than expected in years when a yearling daughter was present. Core areas and centres of activity also changed when the nest site was moved. Most daughters settled on their natal areas. The relinquishing of nest sites by females to their daughters is seen as a form of parental investment. 相似文献
965.
966.
Michaelis-Menten uptake kinetics were observed at all light intensities. With constant illumination, the Vmax and K1 in nitrate uptake over the natural light intensity range of 0 to 2000 E were 0.343 g-at NO3–N(g)-1 at protein-N h-1 and 26 E, respectively. Nitrate uptake was inhibited at higher light intensities. The Ks for nitrate uptake did not vary as a function of light intensity remaining relatively constant at 0.62 g-at NO3–N 1-1. With intermittent illumination, the Vmzx for light intensity in nitrate uptake over a light intensity range of 0 to 5000 E was 0.341 g-at NO3–N(g)-1-at protein-N h-1. No inhibition of nitrate uptake was observed at higher than natural light intensities. Chaetoceros curvisetus will probably never experience light inhibition of nitrate uptake under natural conditions. 相似文献
967.
Methodology and measurement of adenylate energy charge ratios in environmental samples 总被引:7,自引:0,他引:7
A method for measuring ATP, ADP and AMP levels in environmental samples was devised, and applied to seawater and bacterial cell extracts. This procedure is specifically designed for measuring the extremely low concentrations of total adenine nucleotides ([AT]=[ATP]+[ADP]+[AMP]) that are apt to occur in most natural ecosystems (i.e., 10 ng AT ml-1 of sample extract). Although the current assay methodology can be used with purified firefly luciferase reagents, it has been suitably modified to accept crude luciferase preparations as well. ATP, ADP and AMP levels have been measured, and the corresponding energy charge (EC) ratios determined for seawater samples collected off the Southern California coast. The EC ratios ranged from 0.50 to 0.89, with peak values corresponding to the subsurface maxima in ATP and chlorophyll a concentrations, and the minimum values corresponding to the deepest water sampled (1500 m). The measurement of adenylate energy charge ratios in environmental samples can be a useful indicator of mean community metabolic activity and potential for cell growth. 相似文献
968.
Petrocelis middendorffii is a non-calcareous encrusting red alga growing on rocks in the middle and upper intertidal zone. We have photographically followed the fate of 43 individual plants for periods up to 88 months at Waadah Island, near Neah Bay, Washington (USA). Growth is slow, with surface area increasing about 4% year-1 for those individuals growing. Other plants were observed to decrease in total surface coverage, fragment, and eventually disappear. Plants on neighboring but not contiguous sample sites are not in synchrony, with some subpopulations increasing their total space coverage while others diminished theirs. Age estimates, based on a variety of growth interpretations, suggest that an average sized individual (123 cm2) will be from 25 to 87 years old. Recruitment and mortality rates appear low. The ecological traits ofP. middendorffii may be similar to those of other crustose heteromorphic benthic algae. 相似文献
969.
Blooms of the brevetoxin-producing Karenia brevis in the Gulf of Mexico cause massive fish kills, food poisoning and adverse respiratory effects in humans. Sedimentation of
toxic cells following inert clay application could reduce toxin incorporation by commercially important suspension-feeding
bivalves and thus prevent direct public health impacts, but could potentially lead to brevetoxin (PbTx) accumulation by benthic
deposit-feeders. The goal of this study was therefore to compare suspension- and deposit-feeding as pathways for brevetoxins.
We investigated: (1) the effect of toxic K. brevis on both feeding modes using a facultative deposit-suspension feeding tellinid bivalve, the clam Macoma balthica, as a model species and (2) the relative effectiveness of brevetoxin transfer via suspension- and deposit-feeding over 24-h
exposure. Sedimentation of K. brevis was achieved by treatment with 0.25 g phosphatic clay l−1 and brevetoxin concentrations were measured by ELISA. Karenia brevis reduced both suspension- and deposit-feeding activity. This study demonstrates that brevetoxins can be rapidly accumulated
by a surface deposit-feeding bivalve from sedimented K. brevis cells and that comparable toxin levels can be attained by both suspension- and deposit-feeding modes [1.2–1.6 μg PbTx (g
tissue wet weight)−1]. Deposit-feeding clams generally do not pose a direct threat to humans but may provide a pathway for brevetoxin food web
transfer. 相似文献
970.
In birds, host experience can modify response to parasites but nothing similar is known for insects. We studied two desert tenebrionid beetles, the subsocial host Parastizopus armaticeps and its obligate and ubiquitous brood-(clepto)parasite Eremostibes opacus, which mimics the hosts odour spectrum. Nearly 10% of host burrows in the field (n=214) remain unparasitised even after introducing E. opacus experimentally (n=22). In discrimination tests, 7% of naive host beetles eject the parasite, showing individual variation in olfactory discrimination ability. To test for effects of prior breeding experience, naive (first time) or experienced (second time) breeders with or without prior parasite contact were confined with E. opacus at breeding onset, their behaviour recorded and parasite number counted on breeding completion. More first- than second-time breeding pairs completely excluded the parasite and second-time breeders with no prior contact with E. opacus had fewer parasites than any others. These pairs increased burrow-guarding dramatically, mostly during early reproduction, this being the best predictor of parasite number. Prolonged guarding, however, must be traded-off against other brood-care behaviours. Hence, it might pay breeding pairs to take the risk of accepting some parasites breeding within their burrow, and we expect this effect will be more pronounced if poor feeding and digging conditions are taken into account. This might explain why apparently 90% of the P. armaticeps breeding burrows in the field contain at least one parasitic E. opacus.Communicated by D. Gwynne 相似文献