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591.
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We present a remarkable chain of events in which percutaneous umbilical cord sampling was performed in an attempt to clarify a situation of possible fetal sex chromosome mosaicism in an amniotic fluid culture and led to the discovery that the mother herself had a 45,X/46,XX/ 47.XXX chromosome constitution. This may have simply represented the chance concurrence of pseudo-mosaicism in the amniotic fluid culture of a woman with an abnormal sex chromosome constitution, but it is also possible that the 45,X colony was maternal in origin. Although clearly a most unusual circumstance, the possibility should be kept in mind when termination of a pregnancy is being considered because of apparent mosaicism in a prenatal diagnostic study. 相似文献
593.
A protocol for easy storage and later expansion of lymphocyte populations is given. Compared with methods using transformed cell lines, the method has a number of advantages for repeated production of cells for the isolation of DNA in amounts sufficient for use in diagnostic DNA technology. 相似文献
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598.
The “Linh Duong” (Pseudonovibos spiralis), a newly described Vietnamese bovid, has been shown to be related to the Caprinae on the basis of the mitochondrial cytochrome
b gene sequencing from horn fragments. To integrate the greatest available biodiversity we performed a new phylogenetic analysis
including all genera of Caprinae. Unexpectedly, Pseudonovibos is found to be robustly associated with Rupicapra, and closer to the Alpine than to the Pyrenean chamois. Several clues led us to interpret this result as a carry-over DNA
contamination of Pseudonovibos by Rupicapra.
Received: 19 July 1999 / Accepted in revised form: 20 October 1999 相似文献
599.
Self-diffusion coefficients were studied for the highly polar liquid N-methylformamide at pressures up to 200 MPa between the melting pressure curves and 420 K by the spin-echo method. N-Methylformamide exists as a mixture of two conformers in the neat liquid. These conformers have large differences at lower
temperatures in their dynamic and structural properties. The self-diffusion coefficient of the cis-conformer being 17% lower than that of the trans-conformer at the same T and p. This is the first observation of such an effect. The experimental study is supported by Monte Carlo (MC) calculations which
show that the first neighbors around a cis conformer are arranged differently than in an all trans liquid. The difference leads in the simulations to a much lower dielectric constant for the trans-cis mixture and might also explain the retardation of diffusion for the cis conformer.
Received: 15 November 1999 / Accepted in revised form: 28 February 2000 相似文献
600.