Evaluation of lot-to-lot repeatability and effect of assay media choice in the recombinant Factor C assay

Measurement of environmental endotoxin exposures is complicated by variability encountered using current biological assay methods arising in part from lot-to-lot variability of the Limulus-amebocyte lysate (LAL) reagents. Therefore, we investigated the lot-to-lot repeatability of commercially available recombinant Factor C (rFC) kits as an alternative to LAL. Specifically, we compared endotoxin estimates obtained from rFC assay of twenty indoor dust samples, using four different extraction and assay media, to endotoxin estimates previously obtained by Limulus amebocyte lysate (LAL) assay and amounts of 3-hydroxy fatty acids (3-OHFA) in lipopolysaccharide (LPS) using gas-chromatography mass spectroscopy (GC-MS). We found that lot-to-lot variability of the rFC assay kits does not significantly alter endotoxin estimates in house dust samples when performed using three of the four assay media tested and that choice of assay media significantly altered endotoxin estimates obtained by rFC assay of house dust samples. Our findings demonstrate lot-to-lot reproducibility of rFC assay of environmental samples and suggest that use of rFC assay performed with Tris buffer or water as the extraction and assay medium for measurement of endotoxin in dust samples may be a suitable choice for developing a standardized methodology.     

Gemeinsame Kontrolle von Zellwachstum und Transportfunktionen in einer Nierenzellinie

Several protein kinases have been shown to be involved in the regulation of cell growth and differentiation. Molecules, regulating the activity of these protein kinases, also effect the activity of certain transport systems. Genetic experiments, suggesting a similar connection between the regulation of cell growth and transport functions in MDCK cells, are discussed.     

RCRA corrective action costs: An estimating model for nonfederal facilities

This article presents an analysis of the prospective costs of RCRA corrective action for private, nonfederal facilities. Two data bases developed by Research Triangle Institute and a remedial action cost model developed by CH2M Hill provide the foundation for this work. The methodology has two components, a remedial action knowledge base and a discrete-state Monte Carlo analysis. Under base case assumptions, it is estimated that the total costs of RCRA corrective action will be $240 billion, with a 5 percent chance the costs will be less than $170 billion and a 5 percent chance the costs will be more than $377 billion.     

Potential of carbon accumulation in no-till soils with intensive use and cover crops in southern Brazil

The area under no-till (NT) in Brazil reached 22 million ha in 2004-2005, of which approximately 45% was located in the southern states. From the 1970s to the mid-1980s, this region was a source of carbon dioxide to the atmosphere due to decrease of soil carbon (C) stocks and high consumption of fuel by intensive tillage. Since then, NT has partially restored the soil C lost and reduced the consumption of fossil fuels. To assess the potential of C accumulation in NT soils, four long-term experiments (7-19 yr) in subtropical soils (Paleudult, Paleudalf, and Hapludox) varying in soil texture (87-760 g kg(-1) of clay) in agroecologic southern Brazil zones (central region, northwest basaltic plateau in Rio Grande Sul, and west basaltic plateau in Santa Catarina) and with different cropping systems (soybean and maize) were investigated. The lability of soil organic matter (SOM) was calculated as the ratio of total organic carbon (TOC) to particulate organic carbon (POC), and the role of physical protection on stability of SOM was evaluated. In general, TOC and POC stocks in native grass correlated closely with clay content. Conversely, there was no clear effect of soil texture on C accumulation rates in NT soils, which ranged from 0.12 to 0.59 Mg ha(-1) yr(-1). The C accumulation was higher in NT than in conventional-till (CT) soils. The legume cover crops pigeon pea [Cajanus cajan (L.) Millsp] and velvet beans (Stizolobium cinereum Piper & Tracy) in NT maize cropping systems had the highest C accumulation rates (0.38-0.59 Mg ha(-1) yr(-1)). The intensive cropping systems also were effective in increasing the C accumulation rates in NT soils (0.25-0.34 Mg ha(-1) yr(-1)) when compared to the double-crop system used by farmers. These results stress the role of N fixation in improving the tropical and subtropical cropping systems. The physical protection of SOM within soil aggregates was an important mechanism of C accumulation in the sandy clay loam Paleudult under NT. The cropping system and NT effects on C stocks were attributed to an increase in the lability of SOM, as evidenced by the higher POC to TOC ratio, which is very important to C and energy flux through the soil.     

Gas Chromatographic Analysis of Incinerator Effluents

Comparative gas cleaning performance of a pilot-scale venturi scrubber with throat dimensions of 6 in. wide × 1 2 in. long × 1 2 in. deep was obtained for the following three methods of water injection: a system of spray nozzles located along each short side of the throat (Figure 2a); a continuous slot located immediately above the nozzles, along each short side of the throat (Figure 2b); and a weir located 2 ft above the spray nozzles along long side of the throat (Figure 2c). For each method of water injection the gas cleaning performance, as a function of the pressure drop, was measured by two tests: absorption of SO₂ and collection efficiency for particles of methylene blue of controlled size.