全文获取类型
收费全文 | 30372篇 |
免费 | 344篇 |
国内免费 | 457篇 |
专业分类
安全科学 | 951篇 |
废物处理 | 1440篇 |
环保管理 | 3675篇 |
综合类 | 5069篇 |
基础理论 | 7958篇 |
环境理论 | 18篇 |
污染及防治 | 8028篇 |
评价与监测 | 2071篇 |
社会与环境 | 1785篇 |
灾害及防治 | 178篇 |
出版年
2023年 | 140篇 |
2022年 | 322篇 |
2021年 | 316篇 |
2020年 | 240篇 |
2019年 | 284篇 |
2018年 | 478篇 |
2017年 | 478篇 |
2016年 | 742篇 |
2015年 | 568篇 |
2014年 | 886篇 |
2013年 | 2416篇 |
2012年 | 1060篇 |
2011年 | 1436篇 |
2010年 | 1168篇 |
2009年 | 1200篇 |
2008年 | 1439篇 |
2007年 | 1485篇 |
2006年 | 1264篇 |
2005年 | 1096篇 |
2004年 | 984篇 |
2003年 | 1077篇 |
2002年 | 951篇 |
2001年 | 1238篇 |
2000年 | 863篇 |
1999年 | 517篇 |
1998年 | 348篇 |
1997年 | 358篇 |
1996年 | 356篇 |
1995年 | 423篇 |
1994年 | 446篇 |
1993年 | 352篇 |
1992年 | 374篇 |
1991年 | 352篇 |
1990年 | 386篇 |
1989年 | 341篇 |
1988年 | 296篇 |
1987年 | 278篇 |
1986年 | 223篇 |
1985年 | 248篇 |
1984年 | 265篇 |
1983年 | 256篇 |
1982年 | 241篇 |
1981年 | 222篇 |
1980年 | 175篇 |
1979年 | 194篇 |
1978年 | 176篇 |
1975年 | 140篇 |
1974年 | 117篇 |
1972年 | 130篇 |
1971年 | 131篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
Dedicated to Professor H. J. Berthold, Hannover, on the occasion of his 65th birthday. 相似文献
992.
993.
994.
995.
Prenatal cytogenetic diagnosis after transabdominal chorionic villus sampling in the first trimester
First trimester prenatal cytogenetic diagnosis was attempted in 350 pregnancies after trans-abdominal chorionic villus sampling. The cytogenetic investigation was performed using both a short-term method (24 h incubation) and cell culture. Adequate samples were obtained in 99·1 per cent and in all these cases the fetal karyotype was established. A chromosome abnormality was found in 2·0 per cent of cases. A discrepancy between the karyotype obtained after 24 h incubation and the karyotype in cell culture was observed in 2·3 per cent. Maternal cell contamination in the cultures was confirmed in 13 of 181 cases where the 24 h incubation revealed a male karyotype. Studies of culture morphology showed that colonies of convoluted cells may serve as a marker for contamination with maternal cells in culture. For the present, we recommend using a short-term method as well as cell culture for cytogenetic investigation until the problems with karyotype discrepancy and maternal cell contamination have been further clarified. 相似文献
996.
Pregnancies with fetal trisomy 21 have been associated with low amniotic fluid alpha-fetoprotein levels (AFAFP). This observation led to the suggestion that low AFAFP levels be used as a criterion for completion of a chromosomal analysis in patients who are not otherwise at increased risk for a fetal chromosome abnormality and in whom karyotyping might not have been completed for economic reasons. In order to assess the usefulness of such criteria, we reviewed the AFAFP levels of 90 cases of fetal trisomy 21, 23 cases of trisomy 18, and 10 cases of trisomy 13. These were compared with 2400 control samples with normal chromosome constitution. AFAFP levels were generally lower in pregnancies with trisomy 21, showing a median value of 0·72 MoM. However, 40 per cent of the trisomy 21 samples had AFAFP values greater than 0·8 MoM and 20 per cent were over 1·0 MoM. These data imply that over 50 per cent of Down syndrome cases might have been missed using a cut-off level of 0·70 MoM for completion of chromosome analysis. Using a higher cut-off level will leave only a small percentage of samples unkaryotyped. The distribution of AFP levels in trisomy 13 and 18 is no different from controls; we therefore believe that fetal karyotyping should be completed in every amniotic fluid sample obtained. 相似文献
997.
A. H. J. T. Bröcker-Vriends M.D. E. Briët H. H. H. Kanhai E. Bakker J. C. F. M. Dreesen N. J. Leschot J. J. P. van de Kamp P. L. Pearson 《黑龙江环境通报》1988,8(6):411-421
We evaluated the feasibility, reliability, and acceptability of prenatal diagnosis of haemophilia A by DNA analysis of chorionic villi. Twenty-two women at risk to transmit the abnormal gene were referred for prenatal diagnosis, two of them twice. Two of the 22 women appeared to be non-carriers by DNA analysis. In one of these women, the results were known only after chorionic villus sampling had been carried out. Thirteen of the twenty carriers were heterozygous for an intragenic (Bell or Xbal) marker; six women were only heterozygous for the extragenic DXS52 (Stl4) locus. One of the women was homozygous for all the presently known DNA markers within or closely linked with the factor VIII locus. Twelve of the 22 fetuses at risk were male, ten were female. Seven of the 12 male fetuses were shown to be affected and were subsequently aborted. Four male fetuses appeared to be not affected. In one case, the diagnosis was made by use of an extragenic marker. The woman rejected fetal blood sampling to confirm the diagnosis. After birth, a normal factor VIII level was found in three of the four cases. The fourth pregnancy is still continuing. In one of the 12 male fetuses, no diagnosis at the gene level was possible. DNA analysis is expected to provide maximum certainty as to the phenotype of the fetus for approximately 60 per cent of the women; for another 37 per cent a rate of misdiagnosis of 4–5 per cent applies. In only 3 per cent of the cases will no diagnosis at the gene level be possible as yet. The new possibility of a prenatal diagnosis in the first trimester of pregnancy enabled some of these women to have a family of their own and was appreciated in particular by the women who underwent fetoscopy in an earlier pregnancy. 相似文献
998.
Marcy C. Speer Margaret A. Pericak-Vance Larry H. Yamaoka James Koh Wu-Yen Hung Peter C. Gaskell Jr. Jeffery M. Vance Richard J. Bartlett Allen D. Roses 《黑龙江环境通报》1988,8(6):427-437
Accurate carrier testing and prenatal diagnosis in Duchenne muscular dystrophy (DMD) families is facilitated when an Xp21 deletion is found to be segregating within a family. We discuss the results of the DNA testing in two families, one in which DNA from affected males was available for study and the other in which no DNA from an affected male was available. Factors complicating the counselling of DMD deletion families are outlined. 相似文献
999.
The prenatal diagnosis by ultrasound of an unusual case of supernumerary head is reported. The problems of differential diagnosis, the pathological findings after voluntary interruption of the pregnancy, and the problems of obstetric management are presented. 相似文献
1000.
A syncytiotrophoblast-associated antigen identified by the monoclonal antibody (McAb) H315 is detectable on the surface of a low proportion of peripheral blood cells in pregnant women, raising the possibility of a new approach to prenatal diagnosis of genetic disorders. We aimed at verifying the trophoblastic origin of H315+ cells and their use for prenatal diagnosis of β-thalassaemia. H315 + cells were separated from the peripheral blood of pregnant women: the DNA obtained from these cells in two selected cases was shown to have genetic markers indistinguishable from those of the mother and definitely different from the fetus. Our results suggest that H315 antigen is expressed by maternal cells and that prenatal diagnosis on peripheral blood of the mother using H315 McAb is not feasible. 相似文献