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排序方式: 共有493条查询结果,搜索用时 15 毫秒
21.
Michelle L. Soupir Saied Mostaghimi Nancy G. Love 《Journal of the American Water Resources Association》2008,44(6):1591-1599
Abstract: Remediation of waters impaired by bacterial indicators is usually dictated by total maximum daily load plans, which are heavily dependent on fate and transport modeling of bacterial indicators. Nonpoint source pollution models are most frequently used to assess bacterial transport to surface waters and most models typically simulate bacterial transport as a dissolved pollutant. Previous studies have found that cells preferentially attach to sediments; however, a variety of techniques have been used to assess attachment including filtration, fractional filtration, and centrifugation. In addition, a variety of chemical and physical dispersion techniques are used to release attached and bioflocculated cells from particulates. Here we developed and validated an easy‐to‐replicate laboratory procedure for separation of unattached from attached E. coli which will also identify particle sizes to which E. coli preferentially attach. Physical and chemical dispersion techniques were evaluated and a combined hand shaker treatment for 10 min followed by dilutions in 1,000 mg/l of Tween 85 significantly increased total E. coli concentrations by 31% when compared with a control. In order to separate unattached from attached fractions, two commonly used techniques, fractional filtration, and centrifugation were combined. The filtration and centrifugation treatments did not reduce E. coli concentrations when compared with a control (p > 0.05), indicating that damage was not inflicted upon the E. coli cells during the separation procedure. 相似文献
22.
Cadmium stress alters gene expression of DNA mismatch repair related genes in Arabidopsis seedlings 总被引:1,自引:0,他引:1
Cadmium (Cd) is a non essential element, and is a widespread environmental pollutant. Exposure to Cd can result in a variety of adverse health effects in plant and humans. In the current study, Arabidopsis seedlings were used as a bio-indicator of Cd pollution. Seedlings were grown on MS media containing 0-6.0 mg L(-1) Cd for 18 days, and the gene expression patterns were used to link increased Cd exposure with progressive biological effects. Reduction of total soluble protein content in shoots of the Arabidopsis seedlings occurred with increase in Cd concentrations. For the gene expression patterns, seven genes known to be involved in cell division and DNA mismatch repair (MMR) system were investigated by semi-quantitative RT-PCR, and normalized using 18S rRNA gene expression. Expression of the proliferating cell nuclear antigen 2 (atPCNA 2), MutS 3 homolog (atMSH 3) and MutL1 homolog (atMLH1) genes in shoots of Arabidopsis was strongly induced by exposure to 0.75 mg L(-1) Cd, but were repressed by other Cd concentrations whereas exposure to 0.75-6 mg L(-1) of Cd resulted in a decreased expression of atPCNA1, atMSH 2, 6 and 7 genes independently of any observable biological effects, including survival, fresh weight and chlorophyll level of shoots. This work demonstrated that specific gene expression changes could serve as useful molecular biomarkers indicative of Cd exposure and related biological effects. 相似文献
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Temporal and Spatial Trends in Nutrient and Sediment Loading to Lake Tahoe,California‐Nevada,USA 下载免费PDF全文
Robert Coats Jack Lewis Nancy Alvarez Patricia Arneson 《Journal of the American Water Resources Association》2016,52(6):1347-1365
Since 1980, the Lake Tahoe Interagency Monitoring Program (LTIMP) has provided stream‐discharge and water quality data—nitrogen (N), phosphorus (P), and suspended sediment—at more than 20 stations in Lake Tahoe Basin streams. To characterize the temporal and spatial patterns in nutrient and sediment loading to the lake, and improve the usefulness of the program and the existing database, we have (1) identified and corrected for sources of bias in the water quality database; (2) generated synthetic datasets for sediments and nutrients, and resampled to compare the accuracy and precision of different load calculation models; (3) using the best models, recalculated total annual loads over the period of record; (4) regressed total loads against total annual and annual maximum daily discharge, and tested for time trends in the residuals; (5) compared loads for different forms of N and P; and (6) tested constituent loads against land use‐land cover (LULC) variables using multiple regression. The results show (1) N and P loads are dominated by organic N and particulate P; (2) there are significant long‐term downward trends in some constituent loads of some streams; and (3) anthropogenic impervious surface is the most important LULC variable influencing water quality in basin streams. Many of our recommendations for changes in water quality monitoring and load calculation methods have been adopted by the LTIMP. 相似文献
26.
Michael Shapiro Peter Rogers 《Journal of the American Water Resources Association》1978,14(6):1340-1356
ABSTRACT: The density and distribution of land uses has important consequences for the planning of sewerage systems and for the costs of these systems. This paper examines these consequences using a simplified service area model. The model determines the area to be served by a central waste treatment plant, where alternative on-lot disposal systems are also available. The model is applied to various urban area configurations, which are summarized by their total populations and by their population density distributions. Both minimum regional cost and minimum local cost service area configurations are determined. In addition, the sensitivity of the model to the parameters of the cost and population density functions is assessed. It is found that the model is most sensitive to the parameters of the collection cost function. 相似文献
27.
Employing in-depth, elite interviews, this empirical research contributes to understanding the dynamics among policy windows,
policy change, and organizational learning. First, although much of the research on agenda setting—how issues attract enough
attention that action is taken to address them—has been conducted at the national scale, this work explores the subnational,
regional scale. With decentralization, regional-scale environmental decision-making has become increasingly important. Second,
this research highlights the role of policy windows and instances of related organizational learning identified by natural
resources managers. Having practitioners identify focusing events contrasts with the more typical approach of the researcher
identifying a particular focusing event or events to investigate. A focusing event is a sudden, exceptional experience that,
because of how it leads to harm or exposes the prospect for great devastation, is perceived as the impetus for policy change. 相似文献
28.
Runion GB Davis MA Pritchard SG Prior SA Mitchell RJ Torbert HA Rogers HH Dute RR 《Journal of environmental quality》2006,35(4):1478-1486
Plant species vary in response to atmospheric CO2 concentration due to differences in physiology, morphology, phenology, and symbiotic relationships. These differences make it very difficult to predict how plant communities will respond to elevated CO2. Such information is critical to furthering our understanding of community and ecosystem responses to global climate change. To determine how a simple plant community might respond to elevated CO2, a model regenerating longleaf pine community composed of five species was exposed to two CO2 regimes (ambient, 365 micromol mol(-1) and elevated, 720 micromol mol(-1)) for 3 yr. Total above- and belowground biomass was 70 and 49% greater, respectively, in CO2-enriched plots. Carbon (C) content followed a response pattern similar to biomass, resulting in a significant increase of 13.8 Mg C ha(-1) under elevated CO2. Responses of individual species, however, varied. Longleaf pine (Pinus palustris Mill.) was primarily responsible for the positive response to CO2 enrichment. Wiregrass (Aristida stricta Michx.), rattlebox (Crotalaria rotundifolia Walt. Ex Gmel.), and butterfly weed (Asclepias tuberosa L.) exhibited negative above- and belowground biomass responses to elevated CO2, while sand post oak (Quercus margaretta Ashe) did not differ significantly between CO2 treatments. As with pine, C content followed patterns similar to biomass. Elevated CO2 resulted in alterations in community structure. Longleaf pine comprised 88% of total biomass in CO2-enriched plots, but only 76% in ambient plots. In contrast, wiregrass, rattlebox, and butterfly weed comprised 19% in ambient CO2 plots, but only 8% under high CO2. Therefore, while longleaf pine may perform well in a high CO2 world, other members of this community may not compete as well, which could alter community function. Effects of elevated CO2 on plant communities are complex, dynamic, and difficult to predict, clearly demonstrating the need for more research in this important area of global change science. 相似文献
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William F. Porter Nancy E. Mathews H. Brian Underwood Richard W. Sage Jr. Donald F. Behrend 《Environmental management》1991,15(6):809-814
Populations of white-tailed deer (Odocoileus virginianus) inhabiting many state and national parks and suburban areas have grown to the point that they conflict with human activities.
Conflicts range from destruction of vegetation through browsing to public perception that diseases carried by deer pose threats
to human health. Traditional modes of hunting to control populations are inappropriate in many of these areas because of intense
human development and activity. This article explores an alternative approach for population reduction based on deer social
organization. Female white-tailed deer are highly philopatric and female offspring remain near their dams for life. This suggests
that a population expands slowly as a series of overlapping home ranges in a form analogous to the petals on a rose. Incorporating
the rose petal concept into a model of population growth shows that removal of deer by family unit can potentially alleviate
conflicts in localized areas for as many as 10–15 yr. 相似文献