Safety evaluation of cable barriers installation on rural highways in British Columbia

Objective: The objective of this study was to evaluate the safety effectiveness of cable barrier systems installation on rural highway sections in British Columbia, Canada.

Methods: Data on police-attended serious collisions (injury?+?fatality) on a number of rural highway sections in British Columbia, Canada, were used in the analysis. An empirical Bayes (EB) approach was employed to ensure that the evaluation results were reliable and to account for the regression to the mean artifact. Safety performance functions (SPFs) were developed using data collected at similar sites. For both median cable barrier (MCB) and roadside cable barrier (RCB) sections, the evaluation was undertaken using all serious collisions, truck serious collisions, and off-road serious collisions.

Results: For MCB sections, the evaluation results showed statistically significant reductions of 21.7, 53.8, and 34.8% in all serious collisions, truck serious collisions, and off-road left (ORL) combined with head-on (HO) serious collisions. For RCB sections, statistically significant reductions of 74.7, 100, and 100% were found in all serious collisions, truck serious collisions, and off-road right (ORR) serious collisions, respectively. The impact of the after period on the evaluation results was explored. It was found that the changes in safety become more stable using an after period of 2–5 years.

Conclusions: Cable barriers were successful in reducing the frequency of serious collisions on provincial highways in British Columbia.     

Detection of fetal cells in transcervical samples and prenatal diagnosis of chromosomal abnormalities

Transcervical samples collected by lavage, aspiration, and cytobrush from women between 6 and 13 weeks of gestation were tested for the presence of fetal cells using fluorescence in situ hybridization (FISH) with probes for chromosomes X, Y, 1, and 21, and by polymerase chain reaction (PCR) amplification of DNA sequences derived from chromosomes X, Y, and 21. With a few exceptions, a good correlation was observed between the results of sexing the fetuses using FISH or PCR on transcervical cell (TCC) samples retrieved by lavage and those obtained by testing fetal (placental) tissue. In a comparative study between TCC samples collected by lavage or cytobrush, the sex of the fetus was correctly diagnosed by PCR amplification of a Y-derived DNA sequence. Variable results were observed with samples obtained by aspiration, mainly because this procedure was found to be more prone to failure to remove thick mucus without previous injection of physiological saline. Chromosome 21-derived small tandem repeats (STRs) of fetal origin were successfully detected in about 40 per cent of TCC samples recovered by lavage. Two cases of chromosomal abnormalities, one of trisomy 21 and one of triploidy, were detected in TCC samples in the course of our investigations.     

Spatial variation of phosphorus fractionation in the coral reef sediments of Lakshadweep Archipelago,Indian Ocean

Surface sediments were collected from the shore and lagoons of Kavaratti, Kadamat and Agatti islands of Lakshadweep Archipelago during May 2015 and analysed for the spatial distribution of the micronutrient element, phosphorus. Phosphorus was separated by sequential extraction procedure into five fractions – exchangeable (Ex-P), iron bound, (Fe-P), calcium bound (Ca-P), organic and residual fractions (OP) and total phosphorus (TP). The average relative contribution of each P species to TP was: OP?>?Ca –P?>?Ex – P?>?Fe – P. The high concentration of organic and residual phosphorus (87–96%) compared to inorganic phosphorus is particularly evident at stations characterised by higher total phosphorus concentrations. Among the three forms of IP in the sediments, Ca-P was dominant at all stations. The OC/OP ratio ranged from 3 to 163 in the sediments, suggesting that the organic matter in sediments had been subjected to degradation. Hence, the major contribution towards organic and residual phosphorus form is from the residual fraction comprising biologically resistant or non-available phosphorus form composed of refractory materials. The concentration of phosphorus reported in the present study is higher than that of the earlier studies in Lakshadweep, indicating a terrestrial and anthropogenic in?uence on the sediment.     

Development and applications of functional gene microarrays in the analysis of the functional diversity, composition, and structure of microbial communities

Functional gene arrays (FGAs) are a special type of microarrays containing probes for key genes involved in microbial functional processes, such as biogeochemical cycling of carbon, nitrogen, sulfur, phosphorus, and metals, biodegradation of environmental contaminants, energy processing, and stress responses. GeoChips are considered as the most comprehensive FGAs. Experimentally established probe design criteria and a computational pipeline integrating sequence retrieval, probe design and verification, array construction, data analysis, and automatic update are used to develop the GeoChip technology. GeoChip has been systematically evaluated and demonstrated to be a powerful tool for rapid, specific, sensitive, and quantitative analysis of microbial communities in a high-throughput manner. Several generations of GeoChip have been developed and applied to investigate the functional diversity, composition, structure, function, and dynamics of a variety of microbial communities from different habitats, such as water, soil, marine, bioreactor, human microbiome, and extreme ecosystems. GeoChip is able to address fundamental questions related to global change, bioenergy, bioremediation, agricultural operation, land use, human health, environmental restoration, and ecological theories and to link the microbial community structure to environmental factors and ecosystem functioning.     

Measurement of particulate aliphatic and polynuclear aromatic hydrocarbons in Santiago de Chile: source reconciliation and evaluation of sampling artifacts

Using a novel sampler, particulate organic compounds were collected in Santiago de Chile from June 9 to August 10, 1997. This sampler consists of a diffusion denuder to remove gas-phase organics prior to particle collection, a Teflon filter, and a PUF cartridge downstream of the filter. PAHs and n-alkanes were measured using gas chromatography/mass spectrometry analysis. Volatilization of particles collected on the Teflon filter varied from 15 to 85% for both n-alkanes and PAHs, with strong dependence on molecular weight. The relative distribution of n-alkanes and the values of molecular diagnostic ratios, such as Carbon preference index, indicated a mixed origin with strong anthropogenic input. Indeed, CPI values ranged from 0.66 to 1.96 (for the whole range of n-alkanes). The percent contribution of leaf “wax” n-alkanes (4.55–20.83%) indicated the low contribution of biogenic sources. In addition, the distribution pattern of PAHs was characteristic of anthropogenic emissions. The dominant contribution of combustion-related PAHs (CPAHs), 74–84%, indicated that vehicular emissions was the major source of PAHs.     

Biochemical composition of sedimentary organic matter in the coral reefs of Lakshadweep Archipelago,Indian Ocean

Surface sediments were collected from the shore and lagoons of Kavaratti, Kadamat Agatti and Pitti islands of Lakshadweep Archipelago during May 2015 and analysed for biochemical composition and quality of organic matter. The biochemical composition of sedimentary organic matter from the entire study area was characterised by the dominance of carbohydrates (CHO) followed by proteins (PRT) and finally lipids (LPD). PRT:CHO ratios were less than 1 and indicated the presence of aged organic matter in the islands. The poor nutritional quality of sediments to support benthic fauna was evident from the values of LPD:CHO ratios. The refractory nature of sediments and less availability of food to benthic source was supported by BPC:TOC ratios. Based on estimated ratios and biopolymeric carbon values, the trophic status of the study area was categorised as oligotrophic.     

Evaluation of various global solar radiation models for Nigeria

This study assessed the performance of six solar radiation models. The objective was to determine the most accurate model for estimating global solar radiation on a horizontal surface in Nigeria. Twenty-two years meteorological data sets collected from the Nigerian Meteorological agency and the National Aeronautics and Space Administration for the three regions, covering the entire climatic zones in Nigeria were utilized for calibrating and validating the selected models for Nigeria. The accuracy and applicability of various models were determined for three locations (Abuja, Benin City, and Sokoto), which spread across Nigeria using seven viable statistical indices. This study found that the estimation results of considered models are statistically significant at the 95% confidence level, but their accuracy varies from one location to another. However, the multivariable regression relationship deduced in terms of sunshine ratio, air temperature ratio, maximum air temperature, and cloudiness performs better than other relationships. The multivariable relationship has the least root mean square error and mean absolute bias error, not exceeding 1.0854 and 0.8160 MJ m^?2 day^?1, respectively, and monthly relative percentage error in the range of ± 12% for the study areas.     

A Rapid Method to Score Stream Reaches Based on the Overall Performance of Their Main Ecological Functions

A method was developed to score the ecological condition of first- to third-order stream reaches in the Auckland region of New Zealand based on the performance of their key ecological functions. Such a method is required by consultants and resource managers to quantify the reduction in ecological condition of a modified stream reach relative to its unmodified state. This is a fundamental precursor for the determination of fair environmental compensation for achieving no-net-loss in overall stream ecological value. Field testing and subsequent use of the method indicated that it provides a useful measure of ecological condition related to the performance of stream ecological functions. It is relatively simple to apply compared to a full ecological study, is quick to use, and allows identification of the degree of impairment of each of the key ecological functions. The scoring system was designed so that future improvements in the measurement of stream functions can be incorporated into it. Although the methodology was specifically designed for Auckland streams, the principles can be readily adapted to other regions and stream types.     

Equivalency of a personal dust monitor to the current United States coal mine respirable dust sampler

The United States National Institute for Occupational Safety and Health, through an informal partnership with industry, labor, and the United States Mine Safety and Health Administration, has developed and tested a new instrument known as the Personal Dust Monitor (PDM). The new dust monitor is an integral part of the cap lamp that coal miners normally carry to work and provides continuous information about the concentration of respirable coal mine dust within the breathing zone of that individual. Previous laboratory testing demonstrated that there is a 95% confidence that greater than 95% of individual PDM measurements fall within +/-25% of reference measurements. The work presented in this paper focuses on the relationship between the PDM and respirable dust concentrations currently measured by a coal mine dust personal sampler unit utilizing a 10 mm Dorr-Oliver nylon cyclone. The United Kingdom Mining Research Establishment instrument, used as the basis for coal mine respirable dust standards, had been designed specifically to match the United Kingdom British Medical Research Council (BMRC) criterion. The personal sampler is used with a 1.38 multiplier to convert readings to the BMRC criterion. A stratified random sampling design incorporating a proportionate allocation strategy was used to select a sample of mechanized mining units representative of all US underground coal mines. A sample of 180 mechanized mining units was chosen, representing approximately 20% of the mechanized mining units in production at the time the sample was selected. A total of 129 valid PDM/personal sampler dust sample sets were obtained. A weighted linear regression analysis of this data base shows that, in comparison with the personal sampler, the PDM requires a mass equivalency conversion multiplier of 1.05 [95% C.I.=(1.03, 1.08)] when the small intercept term is removed from the analysis. Removal of the intercept term results in a personal sampler-equivalent concentration increase of 2.9% at a PDM measurement of 2.0 mg m(-3).