Diastereoisomer- and enantiomer-specific determination of hexabromocyclododecane in fish oil for food and feed

Fish oils are one of the main sources of ω-3 fatty acids. However, they can present elevated levels of some lipophilic pollutants, such as hexabromocyclododecanes (HBCDs). Since data about HBCDs in fish oil samples are very limited, in this study, 25 samples of fish oil for feed and food have been analyzed. Total HBCDs, as well as, α-, β- and γ-diastereoisomers have been determined. Total HBCDs ranged from 0.09 to 26.8 ng g⁻¹, with higher concentrations in fish oil for feed (average value of 9.69 ng g⁻¹) than those for food (1.14 ng g⁻¹). Concentrations of α-HBCD (average value of 4.12 ng g⁻¹ in feed samples and 0.48 ng g⁻¹ in food samples) and γ-HBCD (5.05 and 0.43 ng g⁻¹ respectively) were higher than that of β-HBCD (0.52 and 0.19 ng g⁻¹ respectively) in most of the samples. However, none of them was predominant in the samples. Concentrations of HBCDs were compared to concentrations of other pollutants and correlation between dioxin and dioxin-like PCBs levels and HBCDs levels were observed. Intake of HBCDs was calculated for fish oil with human consumption purposes and it ranged from 0.08 to 5.38 ng HBCDs d⁻¹, which could contribute significantly to HBCDs total intake. Enantiomeric fractions were also determined. No clear enrichment was observed for γ-HBCD, while (−)-α-HBCD enrichment was detected in some samples.     

Strain specificity in a tropical marine sponge

Grafting experiments have provided direct evidence for the occurrence of strain specificity in the marine demosponge Verongia longissima (Carter). All autografts accepted, all xenografts rejected, and there was both acceptance and rejection of allografts. Among allografts, groups of individuals could easily be identified which showed consistent acceptances and rejections of other individuals. The groups were designated as strains. Histological preparations of graft rejections showed the development of a cuticle between the surfaces of the two sponges in contact. The role of specificity in patterns of interactions between sponges in situ has been discussed from an ecological perspective.     

Sources and concentrations of indoor nitrogen dioxide in Barcelona, Spain

Sources and concentrations of indoor nitrogen dioxide (NO2) were examined in Barcelona, Spain, during 1996-1999. A total of 340 dwellings of infants participating in a hospital-based cohort study were selected from different areas of the city. Passive filter badges were used for indoor NO2 measurement over 7-30 days. Dwelling inhabitants completed a questionnaire on housing characteristics and smoking habits. Data on outdoor NO2 concentrations were available for the entire period of the study in the areas of the city where indoor concentrations were determined. Bivariate analysis was performed to investigate relationships between indoor NO2 concentrations on one hand and outdoor NO2 concentrations, housing, and occupant characteristics on the other. Stepwise multiple linear regression was performed with variables that were found to have a significant bivariate relationship. Indoor NO2 mean values ranged between 23.57 ppb in 1996 and 27.02 ppb in 1999, with the highest yearly value of 27.82 ppb in 1997. In the same time period, mean outdoor NO2 concentration ranged between 25.26 and 25.78 ppb with a peak of 30.5 ppb in 1998. Multiple regression analysis showed that principal sources of indoor NO2 concentrations were the use of a gas cooker, the absence of an extractor fan when cooking, and cigarette smoking. The absence of central heating was also associated with higher NO2 concentrations. Finally, each ppb increase in outdoor NO2 was associated with a 1% increase in indoor concentrations.     

Influence of tree species composition,thinning intensity and climate change on carbon sequestration in Mediterranean mountain forests: a case study using the CO2Fix model

Prediction of future forest carbon (C) stocks as influenced by forest management and climate is a crucial issue in the search for strategies to mitigate and adapt to global change. It is hard to quantify the long-term effect of specific forest practices on C stocks due to the high number of processes affected by forest management. This work aims to quantify how forest management impacts C stocks in Mediterranean mountain forests based on 25 combinations of site index, tree species composition and thinning intensity in three different climate scenarios using the CO2Fix v.3.2 model Masera et al. (Ecol Modell 164:177–199, 2003). The study area is an ecotonal zone located in Central Spain, and the tree species are Scots pine (Pinus sylvestris L.) and Pyrenean oak (Quercus pyrenaica Willd.). Our results show a strong effect of tree species composition and a negligible effect of thinning intensity. Mixed stands have the highest total stand C stocks: 100 % and 15 % more than pure oak and pine stands respectively, and are here suggested as a feasible and effective mitigation option. Climate change induced a net C loss compared to control scenarios, when reduction in tree growth is taken into account. Mixed stands showed the lowest reduction in forest C stocks due to climate change, indicating that mixed stands are also a valid adaptation strategy. Thus converting from pure to mixed forests would enhance C sequestration under both current and future climate conditions.     

Environmental performance of construction waste: Comparing three scenarios from a case study in Catalonia,Spain

The main objective of this paper is to evaluate environmental impacts of construction wastes in terms of the LIFE 98 ENV/E/351 project. Construction wastes are classified in accordance with the Life Program Environment Directive of the European Commission. Three different scenarios to current waste management from a case study in Catalonia (Spain) have been compared: landfilling, recycling and incineration, and these scenarios were evaluated by means of Life Cycle Assessment. The recommendations of the Catalan Waste Catalogue and the European Waste Catalogue have been taken into account. Also, the influence of transport has been evaluated.Results show that in terms of the Global Warming Potential, the most environmentally friendly treatment was recycling, followed by incineration and lastly landfilling. According to the influence of treatment plants location on the GWP indicator, we observe that incineration and recycling of construction wastes are better than landfilling, even for long distances from the building site to the plants. This is true for most wastes except for the stony types, than should be recycled close to the building site.In summary, data from construction waste of a Catalan case study was evaluated using the well established method of LCA to determine the environmental impacts.     

A 10 μm Outpoint Size Selective Inlet for Hi-Vol Samplers

A Two-stage Size Selective Inlet for use with hi-vol samplers was designed and tested. The inlet, which operates at a flow rate of 1.13 m³/min, is shown to have a cutpoint of 9.8 μm and a fractionation curve slope of 1.45. The cutpoint is well within the EPA suggested limits of 10 ± 1 μm. Fractionation is not affected by wind speed over the test range of 2-24 km/h.

Re-entrainment or bounce of solid particles is not of consequence. The difference in penetration of 20 μm aerodynamic diameter glass beads and liquid aerosols is less than 1% at all wind speeds.     

Modelling soil carbon development in Swedish coniferous forest soils—An uncertainty analysis of parameters and model estimates using the GLUE method

Boreal forest soils such as those in Sweden contain a large active carbon stock. Hence, a relatively small change in this stock can have a major impact on the Swedish national CO₂ balance. Understanding of the uncertainties in the estimations of soil carbon pools is critical for accurately assessing changes in carbon stocks in the national reports to UNFCCC and the Kyoto Protocol. Our objective was to analyse the parameter uncertainties of simulated estimates of the soil organic carbon (SOC) development between 1994 and 2002 in Swedish coniferous forests with the Q model. Both the sensitivity of model parameters and the uncertainties in simulations were assessed. Data of forests with Norway spruce, Scots pine and Lodgepole pine, from the Swedish Forest Soil Inventory (SFSI) were used. Data of 12 Swedish counties were used to calibrate parameter settings; and data from another 11 counties to validate. The “limits of acceptability” within GLUE were set at the 95% confidence interval for the annual, mean measured SOC at county scale. The calibration procedure reduced the parameter uncertainties and reshaped the distributions of the parameters county-specific. The average measured and simulated SOC amounts varied from 60 t C ha⁻¹ in northern to 140 t C ha⁻¹ in the southern Sweden. The calibrated model simulated the soil carbon pool within the limits of acceptability for all calibration counties except for one county during one year. The efficiency of the calibrated model varied strongly; for five out of 12 counties the model estimates agreed well with measurements, for two counties agreement was moderate and for five counties the agreement was poor. The lack of agreement can be explained with the high inter-annual variability of the down-scaled measured SOC estimates and changes in forest areas over time. We conclude that, although we succeed in reducing the uncertainty in the model estimates, calibrating of a regional scale process-oriented model using a national scale dataset is a sensitive balance between introducing and reducing uncertainties. Parameter distributions showed to be scale sensitive and county specific. Further analysis of uncertainties in the methods used for reporting SOC changes to the UNFCCC and Kyoto protocol is recommended.     

Strategic adaptation of nitrogen management for El Niño Southern Oscillation-induced winter wheat system

The rainfall anomaly (RA) associated with El Niño-Southern Oscillation (ENSO) has various unwanted impacts on agricultural system globally. The loss of inorganic nitrogen (N) depending on extreme wet or dry conditions is a major concern. The main objective of this study was to adapt site-specific N strategies to mitigate the effects of ENSO on yields of winter wheat (Triticum aestivum L.; WW) system. After thorough calibration and evaluation, Decision Support Systems for Agrotechnology Transfer (DSSAT, version 4.5) model suite was adapted. Seasonal analysis was used to compare the variability in simulated leached-N, N uptake, and WW yields under long-term historical real-weather conditions. The site X climate interactions and impacts of weather factors on WW yields were assessed across 60 growing seasons in three phases of ENSO: El Niño (EN), La Niña (LN), and neutral (NT). Based on RA, the simulated yields were lower during LN than EN (11 %) and NT (12 %) on coastal sandy-loam soils and higher during LN than EN (13 %) and NT (8 %) on heavy silt loam soils at valley. N strategy with basal of 22 kg N ha^?1?+?a split of 112 kg N ha^?1 at Feekes (F) 4 stage of WW was adapted for maximum yield and minimum N leaching during LN at valley and NT at coastal sites. However, basal of 22 kg N ha^?1?+?two equal splits of 56 kg N ha^?1 at both F4 and F6 was found as the most adaptable N strategy during both EN and NT phases at valley and EN and LN at coastal sites.