In vivo methylation of guanine by the organophosphorus insecticide tetrachlorvinphos

The in vivo methylating capability of the organophosphorus insecticide tetrachlorvinphos, assayed by the formation of 7-methyl-guanine in mouse liver, was investigated. Following intraperitoneal injection of male mice with different doses of the 14C-insecticide, labelled at the OCH3 groups, the total and specific radioactivity of nucleic acids and protein were determined. The 14C-labelling in the isolated macromolecules reached its maximum 24 hours following administration of the insecticide. Analysis of the acid hydrolysate of DNA and of RNA on Dowex-50 WX-12 revealed the presence of (7-14C) methylguanine. At maximum 14C-labelling, the amount of radioactive 7-MeGu, calculated as fraction of total dose, was around 9 X 10(-5) and 39 X 10(-5) for DNA and RNA, respectively.     

Microbial degradation of trifluralin by Aspergillus carneus, Fusarium oxysporum and Trichoderma viride

3H-Trifluralin was synthesized by condensation of 3H-4-chloro-3,5-dinitro-alpha, alpha, alpha-trifluorotoluene with di-n-propylamine. After incubation of trifluralin with Aspergillus carneus, Fusarium oxysporum and Trichoderma viride for 10 days, a small percentage (less than 10%) of unchanged herbicide was recovered in the extractable fraction. This indicates a fairly rapid degradation of the herbicide by the fungal species. Other than trifluralin, the culture medium contained at least five labelled products: 2,6-dinitro-N-n-propyl-alpha, alpha, alpha-trifluoro-p-toluidine; 2,6-dinitro-alpha, alpha, alpha-trifluoro-p-toluidine; 2-amino-6-nitro-alpha, alpha, alpha-trifluoro-p-toluidine, 2,6-dinitro-4-trifluoromethyl phenol and a major polar product which constituted more than 50% of the total extractable transformation products. A pathway, which simulates that of aerobic degradation of the herbicide in soil, is suggested for the microbiological degradation of trifluralin.     

Comparison of nonlinear least squares and log transformation procedures for calculating volatilization coefficients

A nonlinear least squares procedure and a log transformation procedure for calculating first-order rate coefficients from experimental concentration-versus-time data were compared using laboratory measurements of the volatilization from water of 1,1,1-trichloroethane and 1,2-dichloroethane and the absorption of oxygen by water. Ratios of the nonlinear least squares to log transformation volatilization and absorption coefficients for 77 tests ranged from 0.955 to 1.08 and averaged 1.01. Comparison of the maximum, minimum, and mean root-mean-square errors of prediction for six sets of coefficients showed that the errors for the nonlinear least squares procedure were almost always smaller than the errors for the log transformation procedure.     

Seasonal variations of Rn concentrations in the air of a tunnel located in Nagano city

The seasonal variation of 222Rn concentrations in the air of tunnels constructed during World War II at Nagano City has been investigated. The determination of 222Rn concentrations in tunnel air was performed using a solid-state nuclear track detector technique. The monthly radon concentrations changed smoothly, decreasing towards winter and increasing towards summer, and it was found that the concentrations strongly correlate with the temperature difference between the inside and the outside of the tunnel. In the innermost areas of the tunnel, the maximum concentration was observed in July, its value being about 6500 Bq m (-3). The concentrations of radon in the tunnel air decrease exponentially towards the openings of the tunnel, which indicates that the radon concentration in the tunnel is basically governed by diffusion and mixing of radon gas with air. These observations lead to the conclusion that the seasonal variation of the radon concentration in the tunnel air is mainly caused by a convection current due to a stack effect induced by the temperature difference between the tunnel air and the outside air.     

Biochemical composition of the blue crab Callinectes sapidus exposed to the water-soluble fraction of crude oil

The biochemical composition of juvenile blue crabs, Callinectes sapidus, exposed to sublethal concentrations of the water-soluble fraction of South Louisiana crude oil (0 to 2 504 ppb) for 21 d were examined. Although growth took place in all crabs, tissue content varied inversely with exposure concentration while percentage tissue water varied directly with exposure concentration. Total protein, lipid and RNA content of crabs exposed to crude oil were significantly less than that of control crabs by Day 21. DNA content was not significantly different from that of control crabs, suggesting that the difference in tissue content was due to differences in cell volume and not cell number. There were no consistent differences in the concentration of the major biochemical components, indicating that the relative contribution of each of the components remained stable during the period of sublethal stress. Ratios of RNA:DNA and protein:DNA decreased in exposed crabs and were positively correlated with scope for growth and observed growth. The ratios may be useful as indirect indicators of physiological condition. Analysis of lipid classes indicated that structural lipids in stressed crabs were less affected than were lipids used for energy storage. The changes in biochemical composition suggest that the pattern of energy utilization was altered in crabs exposed to crude oil. Growth in size without comparable growth in tissue resulted in decreased tissue content. Available energy was used for growth, with little being stored in lipid reserves.     

Mammalian toxicity of Technical X-factor I. An acute oral study

Intragastric administration of acute doses of Technical X-factor (beta-isomer free BHC, a byproduct obtained during separation of Lindane from Tech. BHC) carried in peanut oil elicited typical signs of central nervous system depression in adult rats. The computed oral LD50 values of X-factor were 4174 and 5673 mg/kg. body weight for male and female rats respectively indicating that its acute toxicity was of relatively low order compared to Tech. BHC. It is suggested that the low acute mammalian toxicity of X-factor could possibly be due to a direct antagonism of the delta isomer (present in larger proportion in X-factor) with the gamma isomer.     

Effects of stream hydraulic conditions on foraging strategies of false dace, Pseudorasbora parva, in the lentic ecosystem

The effects of current velocity on the foraging behavior of false dace, Pseudorasbora parva, were examined in a zero velocity (stagnant) condition, and at four flow rates (5, 7, 11 and 16 cm per second). In stagnant water, the fish displayed a cruise-searching pattern, but they used a drift-feeding foraging tactic in flowing water. The shape of the transverse field of reaction field was elliptical in the stagnant condition, whereas their downward regions were restricted under the flowing conditions. The fish had a blind spot oriented directly in front of their reaction field. Although the relative swimming speed (with considering the against current velocity) increased with increasing flow velocity, the absolute search speed (ignore the against current velocity) showed about 1.2 fish body length per second at stagnant water and low flow velocity. The results of feeding rates showed stop-and wait (driff-feeding) foraging strategy is more efficient in flowing water because prey drift directly towards the fish and prey easily detected.     

Yield and arsenate uptake of arbuscular mycorrhizal tomato colonized by Glomus mosseae BEG167 in As spiked soil under glasshouse conditions

A glasshouse pot experiment was conducted to study the effect of arbuscular mycorrhizal (AM) colonization by Glomus mosseae BEG167 on the yield and arsenate uptake of tomato plants in soil experimentally contaminated with five As levels (0, 25, 50, 75 and 150 mg kg(-1)). Mycorrhizal colonization (50-70% of root length) was little affected by As application and declined only in soil amended with 150 mg As kg(-1). Mycorrhizal colonization increased plant biomass at As application rates of 25, 50 and 75 mg kg(-1). Shoot As concentration increased with increasing As addition up to 50 mg kg(-1) but decreased with mycorrhizal colonization at As addition rates of 75 and 150 mg kg(-1). Shoot As uptake increased with mycorrhizal colonization at most As addition levels studied, but tended to decrease with addition of 150 mg As kg(-1). Total P uptake by mycorrhizal plants was elevated at As rates of 25, 50 and 75 mg kg(-1), and more P was allocated to the roots of mycorrhizal plants. Mycorrhizal plants had higher shoot and root P/As ratios at higher As application rates than did non-mycorrhizal controls. The soil of inoculated treatments had higher available As than uninoculated controls, and higher pH values at As addition levels of 25, 50 and 75 mg kg(-1). Mycorrhizal colonization may have increased plant resistance to potential As toxicity at the highest level of As contamination studied. Mycorrhizal tomato plants may have potential for phytoextraction of As from moderately contaminated soils or phytostabilization of more highly polluted sites.     

Economic Underdevelopment And Sustainable Development In The World:Conditioning Factors,Problems And Opportunities

This paper deals with the various factors that condition underdevelopment in the world. It suggests some alternatives and points out the potential opportunities that both the developed and the underdeveloped world will have in the coming years to change the gloomy prospects that we see today. The present paper outlines the trends in historical priorities in development, changing as they pass from economic development to social development to ecological conservation – the three pillars of Sustainable Development.The paper analyses the importance of geography, a very relevant and often neglected conditioning factor. Also, it analyses the role of socio-economic conditioning factors like political immaturity, demographics, land ownership and external debt.Furthermore, the current opportunities for economic take-off are presented, from cash surpluses to low interest rates, from natural resource management to tourism or migration, from the Information Revolution to liberalisation of agricultural markets. Current obstacles are also analysed. The paper, on the basis of current facts and figures, reaches the conclusion that there is a structural need for sustained development aid for most poor countries, but it must be distributed in a more rational way.Readers should send their comments on this paper to: BhaskarNath@aol.com within 3 months of publication of this issue.     

Synthesis of Molecular Imprinting Polymers Separating Toluic Acid Isomers (II)

Polymers that can separate toluic acid isomers were synthesized by molecular imprinting technique. Molecular imprinting polymers (MIPs) for each isomer of toluic acids (TA) were synthesized using styrene and 4-vinylpyridine (4-Vpy). The adsorption characteristics of TA isomers, salicylic acid (SA), and benzoic acid (BA) on each MIP were investigated. The materials used for polymerization of TA isomers MIPs were adsorbed relatively well. This verifies that the MIPs which can adsorb template selectively were synthesized. In addition, the quantities of adsorbed TA isomers on the TA isomers MIPs and on the control polymer polymerized without template were compared and discussed. The variation of adsorption ability for MIPs with repeated use was investigated, showing excellent reproducibility.