Polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) in breast milk of first-time Irish mothers: impact of the 2008 dioxin incident in Ireland

The 2008 dioxin incident in Ireland resulted in elevated concentrations of polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs) in Irish pork and pork products, due to the consumption of contaminated animal feed by pigs. In order to investigate any resulting impact on the Irish population, these contaminants were measured in pooled breast milk samples from 109 first-time mothers, collected in 2010. A comparison of the results with similar data from 2002 revealed generally lower concentrations of PCDD/Fs and dioxin-like PCBs in the 2010 samples, confirming the declining trend reported by many authors. Contaminant concentration levels for both 2002 and 2010 were generally slightly lower than those reported internationally, with a mean combined PCDD/F and PCB WHO-TEQ of 9.66 pg g⁻¹ fat, for an overall pooled sample of milk from 2010. An apparent slight increase in PCDFs was observed between 2002 and 2010 (from 2.73 pg WHO-TEQ g⁻¹ fat to 3.21 pg WHO-TEQ g⁻¹ fat), with the main contributory congener being 2,3,4,7,8-PentaCDF. While it cannot be totally discounted that the slight increase in 2,3,4,7,8-PentaCDF and in the overall PCDF WHO-TEQ in breast milk could be attributable to consumption of Irish pork during the 2008 incident, we consider that it is more likely that this was due to other factors, including the predominantly urban/industrial sampling locations for the 2010 samples, compared to 2002.     

Evaluation of effectiveness of EDTA and sodium thiosulfate in removing metal toxicity toward sea urchin embryo-larval applying the TIE

Since the development of the TIE (Toxicity Identification and Evaluation) in 1988 it has been assumed that the capacity of EDTA and sodium thiosulfate to complex some metals, and thus remove their toxicity, can be applied to both freshwater and seawater ecotoxicological tests and the results subsequently interpreted. However, it is now known that there is a wide variability in the extent of this complexation. In this context, the removal of toxicity caused by the presence of Hg²⁺, Cd²⁺, Cu²⁺, Cr⁶⁺, Zn²⁺, Ni²⁺, Pb²⁺, Ag¹⁺ and Se²⁺, through metal complexation by EDTA and sodium thiosulfate, in relation to the performance of embryo-larval tests with the sea urchin Arbacia lixula was investigated. It was observed that EDTA was capable of removing the toxicity of Pb²⁺, Zn²⁺ and Cu²⁺ while sodium thiosulfate only reduced the toxicity of Ag¹⁺. Compared to the complexation observed in freshwater ecotoxicological tests, the complexing agents used in this study (EDTA and sodium thiosulfate) have a lower capacity to complex metals in the marine ecotoxicological test with A. lixula.     

Bacterial community profiles from sediments of the Anacostia River using metabolic and molecular analyses

Background aim and scope  

Though the tidal Anacostia River, a highly polluted riverine system, has been well characterized with regard to contaminants, its overall resident bacterial populations have remained largely unknown. Improving the health of this system will rely upon enhanced understanding of the diversity and functions of these communities. Bacterial DNA was extracted from archived (AR, year 2000) and fresh sediments (RE, year 2006) collected from various locations within the Anacostia River. Using a combination of metabolic and molecular techniques, community snapshots of sediment bacterial diversity and activity were produced.     

Fallout traces of the Fukushima NPP accident in southern West Siberia (Novosibirsk,Russia)

Background  

The fallout of artificially produced radioactive isotopes has been recorded at a site in southern West Siberia (54°50′43.6″ N, 083°06′22.4″ E, Novosibirsk, Russia).     

New methodological improvements in the Microtox® solid phase assay

The classic Microtox® solid phase assay (MSPA) based on the inhibition of light production of the marine bacteria recently renamed Aliivibrio fischeri suffers from various bias and interferences, mainly due to physico-chemical characteristics of the tested solid phase. To precisely assess ecotoxicity of sediments, we have developed an alternative method, named Microtox® leachate phase assay (MLPA), in order to measure the action of dissolved pollutants in the aqueous phase. Two hypotheses were formulated to explain the observed difference between MSPA and MLPA results: a real ecotoxicity of the solid phase or the fixation of bacteria to fine particles and/or organic matter. To estimate the latter, flow cytometry analyses were performed with two fluorochromes (known for their ability to stain bacterial DNA), allowing correction of MSPA measurements and generation of new (corrected) IC50. Comparison of results of MLPA with the new IC50 MSPA allows differentiating real ecotoxic and fixation effect in classic MSPA especially for samples with high amount of fines and/or organic matter.     

Enhanced resistance of yeast mutants deficient in low-affinity iron and zinc transporters to stannous-induced toxicity

Tin or stannous (Sn²⁺) compounds are used as catalysts, stabilizers in plastic industries, wood preservatives, agricultural biocides and nuclear medicine. In order to verify the Sn²⁺ up-take and toxicity in yeast cells we utilized a multi-elemental analysis known as particle-induced X-ray emission (PIXE) along with cell survival assays and quantitative real-time PCR. The detection of Sn²⁺ by PIXE was possible only in yeast cells in stationary phase of growth (STAT cells) that survive at 25 mM Sn²⁺ concentration. Yeast cells in exponential phase of growth (LOG cells) tolerate only micro-molar Sn²⁺ concentrations that result in intracellular concentration below of the method detection limit. Our PIXE analysis showed that STAT XV185-14c yeast cells demonstrate a significant loss of intracellular elements such as Mg, Zn, S, Fe and an increase in P levels after 1 h exposure to SnCl₂. The survival assay showed enhanced tolerance of LOG yeast cells lacking the low-affinity iron and zinc transporters to stannous treatment, suggesting the possible involvement in Sn²⁺ uptake. Moreover, our qRT-PCR data showed that Sn²⁺ treatment could generate reactive oxygen species as it induces activation of many stress-response genes, including SOD1, YAP1, and APN1.     

Photocatalytic oxidation of monuron in the suspension of WO3 under the irradiation of UV-visible light

A comprehensive study of the degradation of monuron, one of the phenylurea herbicides, was conducted by UV-Vis/WO₃ process. It was found that hydroxyl radicals played a major role in the decay of monuron while other radicals (e.g. superoxide) and hole might also contribute to the decomposition of monuron. The oxidation path likely plays a major role in the generation of hydroxyl radicals. The effects of initial pH level, initial concentration of monuron, and inorganic oxidants on the performance of UV-Vis/WO₃ process were also investigated and optimized. Comparison between monuron decay pathways by UV-Vis/WO₃ and UV/TiO₂ was conducted. The decay mechanisms, including N-terminus demethylation, dechlorination and direct hydroxylation on benzene ring, were observed to be involved in the oxidation of monuron in these two processes. Sixteen intermediates were identified during the photodegradation of monuron and degradation pathways were proposed accordingly.     

The relationship between the implementation of a Safety Management System and the attitudes of employees towards unsafe acts in aviation

Airports represent highly complex organisations, incorporating such interdependent operations as airlines, ground transport, flight services, ground services, refueling, maintenance, customer services, catering, administration and security. Airports, and especially International Airports, must ensure that their operations are conducted in a safe and efficient manner, as the consequence of any error or failure during operations has the potential for catastrophic outcomes. The international governing body for air transportation, ICAO, requires that airports must implement a Safety Management System as a means of ensuring safe operations and eliminating or reducing the likelihood of low frequency/high consequence incidents. This research project sought to determine the extent to which the implementation of a Safety Management System (SMS) influenced the attitudes of airport employees toward unsafe acts. The hypothesis tested was that the implementation of an SMS into an airport will result in an improvement in attitudes toward safety. A Safety Culture Survey was utilized to measure these attitudes. Two International Airports were chosen to measure the extent to which the introduction of an SMS at one airport would influence the safety attitude and culture of those employees. Sharjah International Airport, UAE, was used as the experimental group, as it did not have a formal SMS in operation, with another English-speaking International Airport (the Second Airport) being used as a control group, as it already had an SMS in operation. A Safety Culture Survey was used as the pre and post-test measure over a 12-month period to determine the extent of influence of the introduction of the SMS at Sharjah Airport. The results of Phase 1 of the survey (June 2008) were compared against the results of Phase 2 of the survey (June 2009). The average score reported by participants at Sharjah Airport increased significantly from pre-test measure to post-test measure in relation to communication, safety rules, supportive environment, personal risk appreciation, work environment, and involvement. At the same time, the average score for personal priorities decreased significantly from Phases 1 to 2. Results indicate that participants at Sharjah Airport recorded a significant positive shift in attitude to the safety factors covered in the Safety Culture Survey, whilst at the same time responses from the Second Airport showed no such shift in attitude. The Second Airport showed neither decline nor improvement in responses. Whilst some methodological issues were identified, the results were of sufficient strength as to conclude that the outcomes reported support the hypothesis that the introduction of an SMS at Sharjah Airport has effected positive changes not observed at the Second Airport. Recommendations for ongoing research were made to further explore the strength of relationship between SMS and safety attitudes, as well as the relationship between safe attitude and safe behaviour.     

Prediction of the confidence interval for stability analysis of chain pillars in coal mines

The conventional deterministic approach based (DAB) methods are simple and widely used to determine an adequate chain pillar size in coal mines where geological settings, coal characterizations and mining methods have been explored. It is evident that such deterministic methods have some intrinsic limitations in handling uncertainties in material properties, non-regular geometries and different mining operations. In the deterministic stability analysis of a chain pillar, the safety factors as well as all input parameters are deterministic. Although, these parameters generally exhibit uncertainty and should lead to a probabilistic expression for the safety factor. The probabilistic analysis provides a confidence interval to express the reliability of chain pillars stability.In this paper, safety factor is defined as a probabilistic function and the failure probability of designed chain pillar of Tabas coal mine is then evaluated using the First Order Second Moment (FOSM) and the Advanced Second Moment (ASM) methods. The results of the probabilistic stability analysis show that the confidence interval for designed pillars with both mentioned methods applied are similar and their values vary from 84% to 88%. The results of such analyses can be used as a basis for decision-making about the size of chain pillars and reduce the risk of pillars stability as well as the uncertainties. However, a trade-off between cost is essential for making the final decision.