Variation of raw wastewater microbiological quality in dry and wet weather conditions

The microbiological quality of urban wastewaters presents important environmental, sanitary, and political challenges. However, the variability of untreated wastewater quality is seldom known when it comes to microbial parameters. This study aims to evaluate the variability of microbiological quality in wastewater influents from different wastewater treatment plants connected to combined and partially separate sewer networks in the Parisian area and to evaluate the impact of this variability on the treatment efficiency and on the accuracy of wastewater effluent monitoring. The densities of fecal indicator bacteria (FIB), Escherichia coli and intestinal enterococci, and their partitioning on settleable particles were analyzed at the inlet of two wastewater treatment plants during dry weather (130 composite samples and 7 days sampled every 2 hours) and storm events (39 composite samples, and 7 rain courses) from 2008 to 2012. The results showed that fecal indicator densities vary according to the network characteristics and according to the meteorological conditions. During storm events, a significant dilution of E. coli and enterococci was observed, as well as a decrease in the settleable fraction of E. coli during the maximal impact of the storm. However, storm events did not significantly impact the regular FIB monitoring. FIB removals by primary and secondary treatment were significantly correlated with FIB densities in influent wastewater; however, meteorological conditions also influenced the removal of FIB.     

Modelling the influence of demographic parameters on group structure in social species with dispersal asymmetry and group fission

Female philopatry characterizes many mammal populations subdivided into social groups. Fission of these social groups is a relatively discrete event in the life of groups or of individuals, leading to the distribution of females among several newly formed groups. Fission is an important event because it can be a way for females to disperse. Group fissions have rarely been observed and their modalities generally remain poorly known, the best-documented species being primates. Most group fissions occur along lines of maternal relatedness, but the death of a matriarch may disrupt the cohesion within a matriline, inducing separation of sisters, accompanied by their descendants, when a group splits. Our model shows that the numbers and sizes of matrilines within groups depend on the precise demographic parameters and age structure of a population and not only on its rate of increase. For comparable population-growth periods, high survival rates of adult females induce an increase in the sizes of matrilines, whereas high survival rates of immature individuals induce an increase in the numbers of matrilines. Following fission, groups of a given size included, in the first case, only a few large matrilines, whereas in the second case, they consisted mainly of many small matrilines. The present study constitutes a preliminary stage, before modelling consequences of demographic structure of groups or populations on their genetic structure.     

Teachers' conceptions of nature and environment in 16 countries

By using an original framework involving complementary statistical approaches, we investigated the environmental attitudes of 6379 pre-service and in-service teachers in 16 countries of Europe and its neighbourhood. To test hypotheses about the nature of environmental attitudes, we examined the variation across groups of individuals (between-class analysis), investigated the independent effects of several candidate explanatory factors (orthogonal analysis), and finally inspected potential relationships between conceptions on a variety of topics (co-inertia analysis).     

Water pressure head and temperature impact on isoxaflutole degradation in crop residues and loamy surface soil under conventional and conservation tillage management

Laboratory incubations were performed in order to evaluate the dissipation of the proherbicide isoxaflutole in seedbed layer soil samples from conventional and conservation tillage systems and in maize and oat residues left at the soil surface under conservation tillage. The effects of temperature and water pressure head on radiolabelled isoxaflutole degradation were studied for each sample for 21 d. Mineralisation of isoxaflutole was low for all samples and ranged from 0.0% to 0.9% of applied ¹⁴C in soil samples and from 0.0% to 2.4% of applied ¹⁴C in residue samples. In soil samples, degradation half-life of isoxaflutole ranged from 9 to 26 h, with significantly higher values under conservation tillage. In residue samples, degradation half-life ranged from 3 to 31 h, with significantly higher values in maize residues, despite a higher mineralisation and bound residue formation than in oat residues. Whatever the sample, most of the applied ¹⁴C remained extractable during the experiment and, after 21 d, less than 15% of applied ¹⁴C were unextractable. This extractable fraction was composed of diketonitrile, benzoic acid derivative and several unidentified metabolites, with one of them accounting for more than 17% of applied ¹⁴C. This study showed that tillage system design, including crop residues management, could help reducing the environmental impacts of isoxaflutole.     

Rational pricing of groundwater with a growing population

When population is increasing, characterizing the optimal water consumption path is complicated by the fact that the underlying dynamics of the water stock is contingent on the level of the stock itself. We propose a method of constructing the optimal path in this case. Since population is increasing, the optimal consumption path may involve refraining at times from consuming the totality of the surface water flow in order to restock in groundwater for future consumption. The aquifer then serves as a means to achieve welfare increasing intertemporal transfers of surface water. Therefore the aquifer itself, as distinct from the stock of water it serves to store, may have value and the marginal valuation of water when groundwater stocks are being drawn upon should, for this reason, differ at times from the marginal valuation of water when it is drawn strictly from surface water.     

Fungal laccases: from structure-activity studies to environmental applications

Laccases are multicopper oxidases mainly secreted by filamentous fungi. Producing radical forms from organic substrates, they are involved in numerous reactions leading to the degradation and polymerization of xenobiotics. Our studies have led to a better knowledge of the structural, catalytic and genetic properties of laccases and allowed to develop a strategy for their evolution through genetic engineering. Here, we show that fungal laccases, wild or engineered, may be potent tools to develop bioremediation processes of soils polluted by organic compounds, and assays to assess the ecotoxicological impact of these pollutants on soil fungi.     

Regulation of worker reproduction in bumblebees (Bombus terrestris): workers eavesdrop on a queen signal

In the annual bumblebee Bombus terrestris, the onset of queen-worker conflict over male production is seasonally and socially constrained. Workers will do better if they start to reproduce (the so-called competition phase) only after ascertaining that larvae are committed to gyne development but before the season ends because they gain more by rearing sister-gynes than their own sons. Here, we tested two nonmutually exclusive hypotheses as to what triggers the onset of worker reproduction: Workers can directly monitor larval development and/or workers eavesdrop on the queen signal that directs gyne development. Exposing workers to gyne larvae through a double mesh did not advance the competition phase compared to control colonies. However, when workers, but not the queen, were allowed contact with gyne larvae, both the competition phase and gyne production were advanced. Thus, while larvae do not emit a volatile pheromone that discloses their developmental route, the physical contact of workers with such larvae triggers early competition phase. However, workers exclusively exposed to worker larvae (colonies prevented from producing gyne larvae) started to reproduce at the same time as control colonies. Replacing the resident queen with an older queen (from gyne-rearing colonies) advanced the competition phase, irrespective of worker age. The results are consistent with the hypothesis that workers eavesdrop on the queen pheromones. This is adaptive because it allows workers a broader time-window for reproduction and thus to gain fitness from rearing both sister-gynes and sons before the season ends without affecting colony development.     

Environmental health in minority and other underserved populations: Benign methods for identifying lead hazards at day care centres of New Orleans

This study tests the hypothesis that exterior sources of lead dust are more important than interior sources in the route of exposure of children. Benign field methods were used to distinguish between potential and actual lead exposure problems. Utilising hand wipe and surface wipe techniques, hand and environmental samples were obtained from selected day care centres at different locations within New Orleans. Previous research has shown that soil lead is determined by location within the city. Private and public day care centres were selected from inner and outer city areas to estimate the extent of hand lead exposure. To measure and identify the extent of environmental lead exposure, hand wipes were taken before and after playing outdoors. Results of preliminary findings show that outdoor lead dust is a more potent contaminant of hands than indoor lead dust. An association was found between the amount of lead on children's hands after playing outdoors and the lead content in the exterior dust and soil. Although two girls out of forty children had exceptionally high hand lead quantities after playing outdoors, in general, boys have higher hand lead levels than girls. The private inner-city day care centre had a severe contamination problem in its outdoor play area. By contrast, the outdoor play area of the public inner city day care centre is of such a high quality that the quantity of lead dust is independent of location in the city.     

Photochimie et environnement - V. Photohydrolyse du monochlorobenzene en solution aqueuse diluee

A primary photohydrolysis of monochlorobenzene into phenol in dilute aqueous solution is occuring quantitatively whatever the experimental conditions were: pH (1<pH<12), excitation wavelength (253.7 nm or 300 nm), presence or absence of oxygen, initial concentration (in the range 10^?4M – 4.10^?3 M). The initial quantum yield of appearance of phenol has been found to be 0.10 ± 0.02 at 253.7 nm, and 0.19 ± 0.06 at 300 nm. The excited state leading to phenol cannot be quenched by acetonitrile at concentration up to 1.9 M. An heterolytic scission of the C-Cl bond parallels the concerted scission of water; a photosubstitution mechanism cannot account for the observed phenomena, especially in acidic media.Monofluoro- and bromobenzene irradiated in degased solution undergo a similar photohydrolysis; the initial quantum yields of appearance of phenol are 0.003 ± 0.001 and 0.06 ± 0.01, respectively for fluoro- and bromobenzene.