Utilizing of Sugar Refinery Waste (Cane Molasses) for Production of Bio-Plastic Under Submerged Fermentation Process

In the present study, depending upon the availability and cheaper cost, different carbon source were tested for the production of PHAs (Polyhydroxyalkonoates) by soil bacterium Pseudomonas aeruginosa and it was found that sugar refinery waste (cane molasses) produced the maximum PHA (biodegradable polymer) which is precursor for bio-plastic development. Urea served as potent nitrogen source over other inorganic nitrogen sources in bio-plastic synthesis. Effect of different physical parameters viz; pH, temperature and agitation speed were also studied on PHA production. Batch cultivation kinetics under optimized cultural and physical condition showed maximum cell mass and PHA concentration of 7.32?±?0.2 and 5.60?±?0.3?g/L, respectively after 54.0?h of cultivation. Sugar refinery waste (Total sugar 4%) and urea (0.8%) improved the economics of the process which exhibited a yield (Y_P/X) of 0.70 with productivity of 0.11?g/L/h. PHA was further characterized as PHB by using Fourier Transform Infra-red Spectroscopy (FT-IR).     

Studies on the removal of nickel from aqueous solutions using modified riverbed sand

This paper highlights the utility of riverbed sand (RS) for the treatment of Ni(II) from aqueous solutions. For enhancement of removal efficiency, RS was modified by simple methods. Raw and modified sands were characterized by scanning electron microscope (SEM), Energy Dispersive Spectroscopy (EDS), and Fourier Transform Infrared Spectroscopy (FTIR) to investigate the effect of modifying the surface of RS. For optimization of various important process parameters, batch mode experiments were conducted by choosing specific parameters such as pH (4.0–8.0), adsorbent dose (1.0–2.0 g), and metal ion concentrations (5–15 mg/L). Removal efficiency decreased from 68.76 to 54.09 % by increasing the concentration of Ni(II) in solution from 5 to 15 mg/L. Removal was found to be highly dependent on pH of aqueous solutions and maximum removal was achieved at pH 8.0. The process of removal follows first-order kinetics, and the value of rate constant was found to be 0.048 min^?1 at 5 mg/L and 25 °C. Value of intraparticle diffusion rate constant (k _id) was found to be 0.021 mg/g min^1/2 at 25 °C. Removal of Ni(II) decreased by increasing temperature which confirms exothermic nature of this system. For equilibrium studies, adsorption data was analyzed by Freundlich and Langmuir models. Thermodynamic studies for the present process were performed by determining the values of ΔG°, ΔH°, and ΔS°. Negative value of ?H° further confirms the exothermic nature of the removal process. The results of the present investigation indicate that modified riverbed sand (MRS) has high potential for the removal of Ni(II) from aqueous solutions, and resultant data can serve as baseline data for designing treatment plants at industrial scale.     

Nanodiagnostics for plant pathogens

Rapid detection technologies with high sensitivity and selectivity for plant pathogens are essential to prevent disease spread and minimize losses to assure optimal productivity and food security. Traditional laboratory techniques such as microscopy and culture are time-consuming, labour intensive and require complex sample handling. Immunological and molecular techniques have advanced but have some issues related to rapidity, signal strength and instrumentation. The integration of immunological and molecular diagnostics with nanotechnology systems offers an option where all detection steps can be accommodated on a portable miniaturized device for rapid and accurate detection of plant pathogens. The sensitive nature of functionalized nanoparticles can be used to design phytopathogen detection devices with smart sensing capabilities for field use. This review summarizes the current status and future prospects of nanotechnology for detection and diagnosis of plant pathogens.     

Tree seedling establishment in dry tropics: an urgent need of interaction studies

The current anthropogenic activities and climate change are increasingly becoming a growing global concern for dry tropical forests. Worldwide, these ecologically important forests have degraded considerably since the past few decades due to such factors. These factors have harmful consequences on the vegetation structure and diversity especially tree seedlings, which may further aggravate climate change. Generally, the vegetation recovery is very slow and unpredictable in the dry tropics due to complex interaction among tree seedling, site (particularly, soil) and climatic conditions. We inculcated that a better understanding of the behavior of individuals of different tree species at seedling stage in dry forests is of immense importance. It is increasingly being recognized for explaining and managing the future composition of plant communities under changing environmental conditions. In this regard, the multi-factorial interaction studies under various resource–disturbance combinations are needed in dry tropical ecosystems to understand the: (1) impact of relative variability in resources and disturbances on the responses of tree seedlings of native species and (2) how the later relates to distinct functional and life history traits of the individual tree species. Most importantly, such studies would improve our limited understanding of how variation (natural and man-made) in nutrient availability, under the influence of other local environmental factors (such as water, light, grass competition, herbivory, fire, allelopathy and enhanced CO₂ conditions), would affect the dynamics of dry tropical forest community. It may help in the proper management of these forests. Moreover, it may prove helpful in the current climate change scenario, as change in forest community dynamics may have consequences on soil C sequestration and CO₂ efflux at global scale.     

An Ecologically-Sustainable Surface Water Withdrawal Framework for Cropland Irrigation: A Case Study in Alabama

Agricultural production in the state of Alabama, USA, is mostly rain-fed, because of which it is vulnerable to drought during growing season. Since Alabama receives a significant portion of its annual precipitation during winter months, the goal of this study was to evaluate the feasibility of water withdrawal from streams during winter months for irrigation in the growing season. The Soil and Water Assessment Tool (SWAT) was used to estimate the quantity of water that can be sustainably withdrawn from streams during winter high flow periods. The model was successfully calibrated and validated for surface runoff, base flow, and total stream flow. The stream flows generated by the model at several locations within the watershed were then used to examine how much water can be sustainably withdrawn from streams of various orders (first, second and third). Although there was a considerable year-to-year variability in the amount of water that can be withdrawn, a 16-year average showed that first, second, and third order streams can irrigate about 11.6, 10.3, and 10.6% of their drainage areas, respectively. The percentage of drainage area that can be irrigated was not a function of stream order.     

Report: Medical students for monitoring biomedical waste segregation practices--why and how? Experience from a medical college

The cornerstone for an effective biomedical waste (BMW) management is appropriate waste segregation. Improper waste segregation practices can have an adverse impact on public health and increase the cost of treatment. Deficiencies in waste segregation practices can be corrected by continuous monitoring and education. Manpower for monitoring waste segregation in a large teaching hospital is scanty. We utilized the undergraduate medical students as monitors and studied the impact on waste segregation practices. A prospective observational study was carried out from August 2004 to January 2005. For monitoring waste segregation, the different areas of the hospital were divided amongst the II M.B.B.S students. They were provided with an observation sheet wherein the status of waste segregation and overall hygiene was recorded on a scale of 1-5. The impact of this programme was then analysed over the study period. The results indicated that a statistically significant improvement in waste segregation practices occurred in all areas. Thus, a large hospital with a medical college can identify students or a similar group for monitoring waste segregation or other aspects of biomedical waste management. This will improve their understanding of good practice in BMW management in future.     

Effective method for extraction of larvicidal component from leaves of Azadirachta indica and Artemisia annua Linn

Leaves of Artemisia annua linn. and Azadirachta indica were extracted in petroleum ether and hexane respectively by different methods of extraction i.e. cold extraction, reflux extraction and soxhlet extraction. The crude extract obtained was tested against third instar larvae of Anopheles stephensi. On comparison of larval mortality of crude extract obtained by these three methods, both soxhlet and reflux extraction method showed 100% mortality at 200 ppm after 48 hr in case of A. annua. However LC50 (20 ppm) value of crude extract obtained by soxhlet extraction showed better results than reflux extraction (35 ppm) method after 72 hr. In case of A. indica, crude obtained by soxhlet showed 100% mortality (after 48 hr) at 250 ppm and LC50 of 69 ppm at 72 hr. Reflux extraction does not show any appreciable mortality even at 250 ppm concentration.     

DDT and HCH residue load in mother's breast milk: a survey of lactating mother's from remote villages in Agra region

Breast milk is the natural and optimal food for the infants. In addition to meeting the nutritional needs, breast milk provides numerous immunological, developmental, psychological, ecological and practical advantages. The levels of the DDT and HCH residues in human milk collected from remote rural part of Agra were analysed. Beta and gamma isomers of HCH accounts for the major residue of total HCH excreted in breast milk. Delta-HCH was not detected in the samples. 95% of the samples were found to be contaminated with DDT and its metabolites. DDD was found in 88% of the samples analyzed. The total concentration of DDT and HCH were found lower than the previous studies carried out in India. The study shows the decreasing levels of these organochlorine pesticides from the environment. Total concentration of total DDT is higher than total HCH.     

Atmospheric dry deposition to leaf surfaces at a rural site of India

Dry deposition flux of major ions (Na+, K+, Ca2+, Mg2+, NH4+, F-, Cl-, NO3- and SO4(2-) to natural surfaces [guava (Psidium guyava) and peepal (Ficus religiosa) leaves] are determined at Rampur, a rural site of semi-arid region of India. Dry deposition flux is the highest for Ca2+ on guava leaves and for NH4+ on peepal leaves. Overall dry deposition flux is higher on guava leaves than of peepal leaves. The variation in deposition flux may be due to surface characteristics (surface roughness) and arrangement of leaves. Peepal leaves are arranged along the axis of the stem, whereas guava leaves are at right angles to the stem. The deposition flux of cations contributes 66% and 76% of dry deposition of all major ions on guava and peepal leaves, respectively as soil is major contributor towards dry deposition flux in tropical regions. ANOVA revealed no significant seasonal difference in deposition, although there is a trend for higher in winter. Deposition velocities of NH4+, NO3- and SO4(2-) are greater on guava leaves than peepal leaves, which can be attributed to the rougher surface of the guava leaf.