Effect of pH and salinity on pathogenicity of Flavobacterium columnare and Myxobacterium sp. in Indian cat fish, Clarias batrachus (Linn.) and Heteropneustes fossilis (Bloch.)

Flavobacterium columnare (FC) and Myxobacterium sp. recorded persistently associated in fish hatchery and culture system of Himalayan and Sub - Himalayan regions were found to be pathogenic. The pH and salinity played a significant role on the pathogenicity of these potent pathogens in case of Clarias batrachus and Heteropneustes fossilis. LD50 value of FC was 10(4.5) CFU in both the fishes and those of Myxobacterium sp it was 10(6) CFU ml(-1) fish(-1). Fish challenged with F. columnare and Myxobacterium sp. (@ 0.2 ml fish(-1)) individually consisting 10(5-6) cfu ml(-1) exhibited explicit symptoms of columnaris disease and marked with ulceration and saddle back lesion on the dorsal side of body. Maximum reisolation of inoculated bacteria was recorded at pH 7.0 and 7.5 and at 0.0-0.5 (F. columnare) and 0.0-1.0% (Myxobacterium sp.) salinity. Foregoing results elucidated that F. columnare was more sensitive to salinity in comparison to Myxobacterium sp. and their pathogenicity significantly (p<0.05) depends on the salinity and pH that might be one of the physical factors to control their proliferation.     

Influence of acetic acid soaking and mechanical grinding treatment on the properties of treated recycled aggregate concrete

Journal of Material Cycles and Waste Management - The utilization of recycled aggregate (RA) extracted from construction and demolition (C&amp;D) waste contributes to reducing the usage of...     

An Effective Means of Biofiltration of Heavy Metal Contaminated Water Bodies Using Aquatic Weed Eichhornia crassipes

Various aquatic plant species are known to accumulate heavy metals through the process of bioaccumulation. World’s most troublesome aquatic weed water hyacinth (Eichhornia crassipes) has been studied for its tendency to bio-accumulate and bio-magnify the heavy metal contaminants present in water bodies. The chemical investigation of plant parts has shown that it accumulates heavy metals like lead (Pb), chromium (Cr), zinc (Zn), manganese (Mn) and copper (Cu) to a large extent. Of all the heavy metals studied Pb, Zn and Mn tend to show greater affinity towards bioaccumulation. The higher concentration of metal in the aquatic weed signifies the biomagnification that lead to filtration of metallic ions from polluted water. The concept that E. crassipes can be used as a natural aquatic treatment system in the uptake of heavy metals is explored.     

An innovative technique for lake management with reference to aeration unit installed at lower lake, Bhopal, India

Water is key element in human life. All forms of life upon the earth depend upon water for their mere existence. Life & water may be aptly said to be two facets of the same coin. Most of the water bodies are getting polluted due to domestic waste, sewage, industrial waste and agricultural effluent. The present study is designed to ascertain the effectiveness of artificial aeration cum Ozonizer unit installed at Lower Lake, Bhopal for assessment of water quality. Various physico- chemical parameters like pH, Dissolved oxygen, Biochemical Oxygen demand, Chemical oxygen demand, nitrate, phosphate and bacteriological status were studied to assess the extent of deterioration in water quality of Lower lake and at the same time to assess the performance of the dual aeration system in improvement of water quality.     

Tectonic evolution of the Aravalli orogen (NW India): an inverted Proterozoic rift basin?

The Aravalli mountain range (AMR) in the northwestern part of the Indian Peninsula consists of two main Proterozoic metasedimentary and metaigneous sequences, the Aravalli and Delhi Supergroups, respectively, which rest over the Archaean gneissic basement. A synthesis and reinterpretation of the available geological, geochronological and geophysical data, including results of own field work and geophysical interpretations pertaining to the AMR, indicate its origin as an inverted basin: rifting into granitoid basement began ca. 2.5; Ga ago with Aravalli passive rifting (ca. 2.5–2.0 Ga) and Delhi active rifting (ca. 1.9–1.6 Ga). Associated mafic igneous rocks show both continental and oceanic tholeiitic geochemistry and are comparable with Phanerozoic, rift-related magmatic products. Available data showed no conclusive evidence for oceanic lithoshere and island-arc/active margin magmatic activity in the AMR. Subsequent inversion and orogeny (Delhi orogeny, ca. 1.5-1.4 Ga) lead to complex deformation and metamorphism. Only in the western and central zones has the basement been involved in this mid-Proterozoic (Delhi) deformation, whereas it is unaffected in the eastern part, except for local shear zones mainly along the basement/cover interface. The grade of metamorphism increases from the greenschist facies in the east to the amphibolite facies in the west with local HP assemblages. These latter are explained by rapid burial and exhumation of thin and cool continental lithosphere. Subsequently, during a final, mild phase of inversion, the Vindhyan basins consisting mainly of sandstones, limestones and shales, flanking the AMR formed which are comparable to foreland basins. The tectonic evolution of the AMR is therefore interpreted as an example of a major inverted continental rift and of a Proterozoic intra-continental orogen.     

Decolorization of synthetic dyes using a copper complex with glucaric acid

Selected azo, acridine, triphenyl methane, anthraquinone and thiazine-based dyes were decolorized using a catalytic system consisting of Cu(II)/glucaric acid/H(2)O(2). More than 90% decolorization was obtained with 100 ppm Acridine Orange, Azure B, Chicago Sky Blue, Crystal Violet, Methyl Orange, Poly B-411, Reactive Black 5, Reactive Blue 2, and Remazol Brilliant Blue R within 24 h. Seventy to eighty percent decolorization was achieved within the first 6 h. The decolorizaton was not affected by pH. The involvement of hydroxyl radicals produced in the system in the decolorization of the dye molecules was confirmed by electron spin resonance study.     

Dust events and their influence on aerosol optical properties over Jaipur in Northwestern India

In this study, we systematically document the link between dust episodes and local scale regional aerosol optical properties over Jaipur located in the vicinity of Thar Desert in the northwestern state of Rajasthan. The seasonal variation of AOT_{500 nm} (aerosol optical thickness) shows high values (0.51?±?0.18) during pre-monsoon (dust dominant) season while low values (0.36?±?0.14) are exhibited during winter. The Ångström wavelength exponent has been found to exhibit low value (<0.25) indicating relative dominance of coarse-mode particles during pre-monsoon season. The AOT increased from 0.36 (April_mean) to 0.575 (May–June_mean). Consequently, volume concentration range increases from April through May–June followed by a sharp decline in July during the first active phase of the monsoon. Significantly high dust storms were observed over Jaipur as indicated by high values of single scattering albedo (SSA_{440 nm}?=?0.89, SSA_{675 nm}?=?0.95, SSA_{870 nm}?=?0.97, SSA_{1,020 nm}?=?0.976) than the previously reported values over IGP region sites. The larger SSA values (more scattering aerosol), especially at longer wavelengths, is due to the abundant dust loading, and is attributed to the measurement site’s proximity to the Thar Desert. The mean and standard deviation in SSA and asymmetry parameter during pre-monsoon season over Jaipur is 0.938?±?0.023 and 0.712?±?0.017 at 675 nm wavelength, respectively. Back-trajectory air mass simulations suggest Thar Desert in northwestern India as the primary source of high aerosols dust loading over Jaipur region as well as contribution by long-range transport from the Arabian Peninsula and Middle East gulf regions, during pre-monsoon season.     

Utilization of anaerobically treated distillery spent wash for production of cellulases under solid-state fermentation

Pollution caused by distillery spent wash on one hand has stimulated the need to develop new technologies to treat the waste and on the other, forced us to reevaluate the efficient utilization of its nutritive potential for production of various high value compounds. In this study, anaerobically treated distillery spent wash was used for the production of cellulases by Aspergillus ellipticus under solid-state fermentation using wheat straw as a substrate. The interactions between distillery effluent concentration, initial pH, moisture content and inoculum size were investigated and modeled using response surface methodology (RSM) involving Box–Behnken design (BBD). Under optimized conditions, filter paper activity, β-glucosidase and endo-β-1,4-glucanase activities were found to be 13.38, 26.68 and 130.92 U/g of substrate respectively. Characterization of endo-β-1,4-glucanase and β-glucosidase was done after partial purification by ammonium sulfate fractionation followed by desalting. The partially purified endo-β-1,4-glucanase and β-glucosidase showed maximum activity at 60 °C. Saccharification studies performed with different lignocellulosic substrates showed that wheat bran was most susceptible to enzymatic hydrolysis. The study suggests that anaerobically treated distillery spent wash can be used as a viable nutrient source for cellulase production under solid-state fermentation by A. ellipticus.     

Pulling force prediction using neural networks

Purpose. In ergonomics and human factors investigations, pulling force (PF) estimation has usually been achieved using various types of biomechanical models, and independent approximation of PF was done with the help of upper extremity joints. Recently, multiple regression methods have gained importance for task-relevant inputs in predicting PF. Artificial neural networks (ANNs) also play a vital role in fitting the data; however, their use in work-related biomechanics and ergonomics is inadequate. Therefore, the current research aimed to accomplish comparative investigation of ANN and regression models by assessing their capacity to predict PF values. Methods. Multipositional PF data were acquired from 200 subjects at three different handle heights and body locations. ANN and regression models were formed using a random sample of three subsets (75% training, 15% selection, 10% validation) for proving the outcomes. Results. The comparison of ANN and regression models shows that the predictions of ANN models had a profoundly explained variance and lower root mean square difference values for the PF data at three handle heights. Conclusions. These outcomes advise that ANNs offer a precise and robust substitute for regression methods, and should be considered a useful method in biomechanics and ergonomics task assessments.