Single-step nested multiplex PCR to differentiate between various bivalve larvae

A nested multiplex polymerase chain reaction (PCR) assay has been developed that allows the discrimination between six bivalve larvae common to Danish coastal waters (Cerastoderma edule, Macoma balthica, Mytilus edulis, Spisula subtruncata, Ensis americanus and members of the order Myoida). This assay involves the simultaneous use of a pair of general universally targeted 18S rRNA gene primers, five specific 18S rRNA gene targeted oligonucleotide primers internal (nested) to the universal primer pair and one species-specific primer that is not nested (Mya). The specificity of each primer was evaluated in silico, empirically, and verified further by sequencing of amplification products from single larvae collected from plankton samples. Identification of individually isolated bivalve larvae from plankton samples was based on the size of the PCR product produced by the specific primers after visualisation by agarose gel electrophoresis. Preliminary studies indicated that this method was suitable for use with freshly collected and preserved larvae, and is therefore suitable for field application.Communicated by M. Kühl, Helsingør     

Changes in the activity budgets of yellow baboons (Papio cynocephalus) during sexual consortships

Summary The energetic cost of guarding estrous females has been assumed to be a potentially major factor in limiting the duration of male consortships in a number of polygamous species. This issue has been examined in the present study by assessing changes in the usual activity budgets of 13 male and 20 female yellow baboons which occurred during their consortships.The percent of time adult males spent feeding was strongly related to their agonistic rank: Higher-ranking males fed proportionately more than lower-ranking males while not in consort, and they showed a relatively greater decrease in feeding time during consortships.The proportion of time females spent feeding was related to their agonistic rank and (to a lesser degree) to their parity. Higher-ranking females fed more than lower-ranking females outside of consortships, and showed a relatively greater decrease in time spent feeding while in consort. Nulliparous females tended to feed more than parous females while not in consort; during consortships, the feeding time of nulliparous females decreased, while that of parous females increased slightly.The activity of male and female consort partners was most coordinated during the fertile cycle stage of the female partner, and least coordinated once the female had passed the fertile stage.Although reductions in feeding time during consortships were generally much more pronounced in males than in females, neither males nor females appeared to attain the undisturbed optimal feeding level for their sex while in consort.     

Bidirectional visibility

The percentage of the cross-sectional area of two objects (e.g., vehicles, hikers, or animals) that can be seen from each of their locations in a forested area is generally not the same. There is a directionality to the visibility between them. This is due to the relative positions and sizes of the vegetation and other view-blocking features between the objects. An analytical technique has been developed to help understand bidirectional visibility. Its use entails the construction of a visibility diagram containing the basic visibility information between observers in a given setting. An example is presented showing the use of the visibility diagram to determine visibility between two moving observers in a forested environment. The diagram is also used to determine the differences in the percentage each observer has of the other's visible cross-sectional area (bidirectional visibility). A discussion of the application of the technique in the planning or development of new facilities, as well as in forest and wildlife management, is provided.     

Body feathers as a potential new biomonitoring tool in raptors: a study on organohalogenated contaminants in different feather types and preen oil of West Greenland white-tailed eagles (Haliaeetus albicilla)

We investigated the variation in concentrations and profiles of various classes of organohalogenated compounds (OHCs) in different feather types, muscle tissue and preen oil from 15 white-tailed eagle (Haliaeetus albicilla) carcasses from Greenland. The influence of moult patterns and potential external contamination onto the feather surface was examined, while the present study is also the first to investigate the use of body feathers for OHC monitoring. Concentrations of sum polychlorinated biphenyls (PCBs) in feathers from white tailed eagles ranged from 2.3 ng/g in a primary wing feather to 4200 ng/g in body feathers. Using 300 mg of body feathers, almost 50 different OHCs could be quantified and median concentrations in body feathers were 10 fold higher than concentrations in tail feathers (rectrices) or primary wing feathers. Body feathers could be very useful for biomonitoring taking into account their easy sampling, short preparation time and high levels of OHCs. In addition, the effects of confounding variables such as feather size, moult and age are also minimised using body feathers. Correlations with concentrations in muscle tissue and preen oil were high and significant for all feather types (r ranging from 0.81 to 0.87 for sum PCBs). Significant differences in concentrations and profiles of OHCs were found between different primary feathers, indicating that the accumulation of OHCs in feathers varies over the moulting period (maximum three years). Washing of feathers with an organic solvent (acetone) resulted in a significant decrease in the measured concentrations of OHCs in feathers. However, our results indicated that preen oil is probably not the only contributor to the external contamination that can be removed by washing with acetone. Possibly dust and other particles may be of importance and may be sticking to the preened feathers. Rectrices washed only with water showed high and significant correlations with concentrations in muscle and preen oil as well. Washing with acetone therefore does not seem to be of great influence when relating to internal tissue concentrations. We recommend washing feathers only with distilled water in order to remove dirt and dust particles before analysis.     

Water supply,waste assimilation,and low-flow issues facing the Southeast Piedmont Interstate-85 urban archipelago

Rapidly growing cities along the Interstate-85 corridor from Atlanta, GA, to Raleigh, NC, rely on small rivers for water supply and waste assimilation. These rivers share commonalities including water supply stress during droughts, seasonally low flows for wastewater dilution, increasing drought and precipitation extremes, downstream eutrophication issues, and high regional aquatic diversity. Further challenges include rapid growth; sprawl that exacerbates water quality and infrastructure issues; water infrastructure that spans numerous counties and municipalities; and large numbers of septic systems. Holistic multi-jurisdiction cooperative water resource planning along with policy and infrastructure modifications is necessary to adapt to population growth and climate. We propose six actions to improve water infrastructure resilience: increase water-use efficiency by municipal, industrial, agricultural, and thermoelectric power sectors; adopt indirect potable reuse or closed loop systems; allow for water sharing during droughts but regulate inter-basin transfers to protect aquatic ecosystems; increase nutrient recovery and reduce discharges of carbon and nutrients in effluents; employ green infrastructure and better stormwater management to reduce nonpoint pollutant loadings and mitigate urban heat island effects; and apply the CRIDA framework to incorporate climate and hydrologic uncertainty into water planning.     

Considering built environment and spatial correlation in modeling pedestrian injury severity

Objective: This study looks at mitigating and aggravating factors that are associated with the injury severity of pedestrians when they have crashes with another road user and overcomes existing limitations in the literature by focusing attention on the built environment and considering spatial correlation across crashes.

Method: Reports for 6,539 pedestrian crashes occurred in Denmark between 2006 and 2015 were merged with geographic information system resources containing detailed information about the built environment and exposure at the crash locations. A linearized spatial logit model estimated the probability of pedestrians sustaining a severe or fatal injury conditional on the occurrence of a crash with another road user.

Results: This study confirms previous findings about older pedestrians and intoxicated pedestrians being the most vulnerable road users and crashes with heavy vehicles and in roads with higher speed limits being related to the most severe outcomes. This study provides novel perspectives by showing positive spatial correlations of crashes with the same severity outcomes and emphasizing the role of the built environment in the proximity of the crash.

Conclusions: This study emphasizes the need for thinking about traffic calming measures, illumination solutions, road maintenance programs, and speed limit reductions. Moreover, this study emphasizes the role of the built environment, because shopping areas, residential areas, and walking traffic density are positively related to a reduction in pedestrian injury severity. Often, these areas have in common a larger pedestrian mass that is more likely to make other road users more aware and attentive, whereas the same does not seem to apply to areas with lower pedestrian density.     

Adaptation to Climate Change in Developing Countries

Adaptation to climate change is given increasing international attention as the confidence in climate change projections is getting higher. Developing countries have specific needs for adaptation due to high vulnerabilities, and they will in this way carry a great part of the global costs of climate change although the rising atmospheric greenhouse gas concentrations are mainly the responsibility of industrialized countries. This article provides a status of climate change adaptation in developing countries. An overview of observed and projected climate change is given, and recent literature on impacts, vulnerability, and adaptation are reviewed, including the emerging focus on mainstreaming of climate change and adaptation in development plans and programs. The article also serves as an introduction to the seven research articles of this special issue on climate change adaptation in developing countries. It is concluded that although many useful steps have been taken in the direction of ensuring adequate adaptation in developing countries, much work still remains to fully understand the drivers of past adaptation efforts, the need for future adaptation, and how to mainstream climate into general development policies.     

Airborne trace element pollution in 11 European cities assessed by exposure of standardised ryegrass cultures

Within a European biomonitoring programme, Italian ryegrass (Lolium multiflorum Lam.) was employed as accumulative bioindicator of airborne trace elements (As, Cd, Cr, Cu, Fe, Ni, Pb, Sb, V, Zn) in urban agglomerations. Applying a highly standardised method, grass cultures were exposed for consecutive periods of four weeks each to ambient air at up to 100 sites in 11 cities during 2000–2002. Results of the 2001 exposure experiments revealed a clear differentiation of trace element pollution within and among local monitoring networks. Pollution was influenced particularly by traffic emissions. Especially Sb, Pb, Cr, Fe, and Cu exhibited a very uneven distribution within the municipal areas with strong accumulation in plants from traffic-exposed sites in the city centres and close to major roads, and moderate to low levels in plants exposed at suburban or rural sites. Accumulation of Ni and V was influenced by other emission sources. The biomonitoring sites located in Spanish city centres featured a much higher pollution load by trace elements than those in other cities of the network, confirming previously reported findings obtained by chemical analyses of dust deposition and aerosols. At some heavily-trafficked sites, legal thresholds for Cu, Pb, and V contents in foodstuff and animal feed were reached or even surpassed. The study confirmed that the standardised grass exposure is a useful and reliable tool to monitor and to assess environmental levels of potentially toxic compounds of particulate matter.     

Windsor, Ontario exposure assessment study: design and methods validation of personal, indoor, and outdoor air pollution monitoring

The Windsor, Ontario Exposure Assessment Study evaluated the contribution of ambient air pollutants to personal and indoor exposures of adults and asthmatic children living in Windsor, Ontario, Canada. In addition, the role of personal, indoor, and outdoor air pollution exposures upon asthmatic children's respiratory health was assessed. Several active and passive sampling methods were applied, or adapted, for personal, indoor, and outdoor residential monitoring of nitrogen dioxide, volatile organic compounds, particulate matter (PM; PM-2.5 pm [PM2.5] and < or =10 microm [PM10] in aerodynamic diameter), elemental carbon, ultrafine particles, ozone, air exchange rates, allergens in settled dust, and particulate-associated metals. Participants completed five consecutive days of monitoring during the winter and summer of 2005 and 2006. During 2006, in addition to undertaking the air pollution measurements, asthmatic children completed respiratory health measurements (including peak flow meter tests and exhaled breath condensate) and tracked respiratory symptoms in a diary. Extensive quality assurance and quality control steps were implemented, including the collocation of instruments at the National Air Pollution Surveillance site operated by Environment Canada and at the Michigan Department of Environmental Quality site in Allen Park, Detroit, MI. During field sampling, duplicate and blank samples were also completed and these data are reported. In total, 50 adults and 51 asthmatic children were recruited to participate, resulting in 922 participant days of data. When comparing the methods used in the study with standard reference methods, field blanks were low and bias was acceptable, with most methods being within 20% of reference methods. Duplicates were typically within less than 10% of each other, indicating that study results can be used with confidence. This paper covers study design, recruitment, methodology, time activity diary, surveys, and quality assurance and control results for the different methods used.