Possible Environmental Factors Underlying Amphibian Decline in Eastern Puerto Rico: Analysis of U.S. Government Data Archives

Abstract: The past three decades have seen major declines in populations of several species of amphibians at high elevations in eastern Puerto Rico, a region unique in the humid tropics because of the degree of environmental monitoring that has taken place through the efforts of U.S. government agencies. I examined changes in environmental conditions by examining time-series data sets that extend back at least into the 1980s, a period when frog populations were declining. The data include forest cover; annual mean, minimum, and maximum daily temperature; annual rainfall; rain and stream chemistry; and atmospheric-dust transport. I examined satellite imagery and air-chemistry samples from a single National Aeronautics and Space Administration aircraft flight across the Caribbean showing patches of pollutants, described as thin sheets or lenses, in the lower troposphere. The main source of these pollutants appeared to be fires from land clearing and deforestation, primarily in Africa. Some pollutant concentrations were high and, in the case of ozone, approached health limits set for urban air. Urban pollution impinging on Puerto Rico, dust generation from Africa ( potential soil pathogens), and tropical forest burning ( gaseous pollutants) have all increased during the last three decades, overlapping the timing of amphibian declines in eastern Puerto Rico. None of the data sets pointed directly to changes so extreme that they might be considered a direct lethal cause of amphibian declines in Puerto Rico. More experimental research is required to link any of these environmental factors to this problem.     

Lower thermal capacity limits of the common brown shrimp (Crangon crangon,L.)

Brown shrimp (Crangon crangon, L.) are subjected to a huge annual temperature range, and certain thermal conditions during winter have been identified to affect the brown shrimp population. Despite that, little is known about its thermal biology with regard to critically low temperatures. In the present study, we determined the critical thermal minima (CT_min) and the critical lethal minima (CL_min) of male and female brown shrimp of different body sizes in laboratory-based experiments. For the CT_min trials, shrimp were acclimated to 4.0, 9.0, and 14.0 °C and exposed to a cooling rate of ?0.2 °C min^?1. In the CL_min trials, brown shrimp were exposed to a cooling rate of ?1.0 °C day^?1 without prior thermal acclimation. Acclimation temperature significantly affected the temperature tolerance of brown shrimp (p < 0.001). CT_min among the experimental groups just varied slightly, and no clear effect of gender or body size was observed. In the CL_min trials, brown shrimp even tolerated the coldest temperature of ?1.7 °C that could be established in the experimental setup. However, we observed a negative relationship between temperature and reactivity within the range of 7.0 and 1.0 °C that was determined by means of the flicking response. This relationship suddenly broke between 1.0 and 0.0 °C where an abrupt drop in the reactivity of the shrimp became apparent. The results of this study revealed that brown shrimp hold a wider thermal range as originally reported and that it can cope with subzero temperatures. Implications of low-temperature tolerance are discussed in the context of the brown shrimp’s ecology as well as stock assessment.     

Factors influencing movement probabilities of big brown bats (Eptesicus fuscus) in buildings.

We investigated movements of female big brown bats (Eptesicus fuscus) roosting in maternity colonies in buildings in Fort Collins, Colorado (USA), during the summers of 2002, 2003, and 2005. This behavior can be of public health concern where bats that may carry diseases (e.g., rabies) move among buildings occupied by people. We used passive integrated transponders (PIT tags) to mark individual bats and hoop PIT readers at emergence points to passively monitor the use of building roosts by marked adult females on a daily basis during the lactation phase of reproduction. Multi-strata models were used to examine movements among roosts in relation to ambient temperatures and ectoparasite loads. Our results suggest that high ambient temperatures influence movements. Numbers of mites (Steatonyssus occidentalis) did not appear to influence movements of female bats among building roosts. In an urban landscape, periods with unusually hot conditions are accompanied by shifting of bats to different buildings or segments of buildings, and this behavior may increase the potential for contact with people in settings where, in comparison to their more regularly used buildings, the bats may be more likely to be of public concern as nuisances or health risks.     

Single-laboratory evaluation and modification of U.S. EPA methods 7470 and 7471 for the determination of mercury in aqueous and solid hazardous wastes

The current U.S. Environmental Protection Agency (U.S. EPA) protocols for mercury determinations in aqueous and solid waste samples (SW-846 Methods 7470 and 7471) using recirculating cold-vapor atomic absorption spectrometry (CV-AAS) have been evaluated. The U.S. EPA methods are not sufficiently flexible to permit special quality control (QC) measures, have limited detectability for low-level mercury concentrations, and are plagued by spectral interferences caused by the nonspecific absorption of primary mercury radiation by volatile organic vapors. The U.S. EPA protocols have been modified in a single-laboratory study to facilitate additional QC measures, to enhance detectability for low-level mercury concentrations, and to eliminate nonspecific vapor absorption interferences. Volumetric manipulations for additional QC measures, if required, are facilitated by performing the sample digestions in Erlenmeyer flasks rather than in the current Biochemical Oxygen Demand (BOD) reduction-aeration bottles. Typical manipulations for additional QC measures that are now feasible include dilution of concentrated samples and multiple aliquot sampling for post-digestion spike and replicate analyses. Instrument detectability is improved 10-fold by using a gas sparging bottle as a dedicated reduction-aeration vessel and a silver wool-amalgamation CV-AAS system operated in an open configuration. The on-line amalgamation/thermal desorption process of the modified CV-AAS system eliminates interfering water and organic matrix vapors prior to the mercury absorption measurement. Good accuracy and precision have been obtained with the amalgamation CV-AAS system for the analyses of four reference sediment materials. The amalgamation CV-AAS measurements on the reference sediment digests have been successfully performed at absolute mercury concentration levels that are only 1 to 4 times above the instrumental detection limit of the U.S. EPA recirculating CV-AAS method.     

Influence of test conditions on solubility controlled leaching predictions from air-pollution-control residues.

Leaching of Al, Ca, Mg, Si, S, Ba, Sr, Mo, Zn, Cd, Pb, and Cu from waste incineration air-pollution-control (APC) residues was investigated. Real-life conditions, i.e. removal of readily soluble compounds and longer equilibration time, were considered. Three different pH-static leaching experiments evaluating the importance of salt level and equilibration time were performed: (i) 48-h test on untreated APC residue samples, (ii) 48-h test on washed residue samples, and finally (iii) a 172-h test on washed residue samples. Experimental data were evaluated by geochemical modelling to identify potential solubility controlling minerals. For some elements (Al, Ca, Mg, Si, S, Mo, Zn, Cd, and Cu) the same controlling minerals were suggested regardless of the equilibration period or untreated/washed character of the tested material, whereas leaching of other elements (Ba, Sr) was far better described by considering a longer equilibration time, thereby pointing out the kinetic effects. Finally, a significant fraction of total lead (57%) was found to be rather mobile in the initial stage of leaching. Both pre-washing of the residues and longer equilibration times were shown to be simple yet useful methods to identify mineral phases that could control the release of constituents after the removal of readily soluble compounds in the initial stage of leaching.     

Noise susceptibility: A comparison of two naval aviator populations

The identification of characteristics of noise-susceptible (S) or noise-resistant (R) individuals is necessary for the development of noise-susceptibility risk profiles. Fifty-six naval aviators, categorized as having either incurred a hearing loss, i.e., hearing threshold levels (HTLs) > 40 dB at 4–8 kHz or retained normal hearing, i.e., HTLs ≤ 25 dB at 125 Hz-8 kHz, after thousands of flight hours were compared along several auditory and non-auditory dimensions. A number of variables occurred differentially in the two groups: Minimal Auditory Intensity Differential (MAID) scores at 2 kHz (p < 0.01) and 4 kHz (p < 0.001); iris pigmentation (blue eyes were over-represented in the S group; p < 0.05); systolic blood pressure (sitting; S group was higher; p < 0.05); calcium, albumin, and LDH levels (higher in the R group; p < 0.05); and present tobacco usage (more S aviators were currently smokers; p < 0.05). The S population also tended (p < 0.10) to exhibit elevated cholesterol and triglyceride levels as well as higher contralateral acoustic reflexes, and to have fewer individuals who had never smoked. Although no classic profile of the S or R individual definitely emerged, results suggested that at least one measurement device (MAID test) may serve as an “early warning” of imminent noise-induced damage. Further research, however, is required to test this possibility.