SMALL FISH MODELS FOR IDENTIFYING CARCINOGENS IN THE AQUEOUS ENVIRONMENT1

ABSTRACT: Contaminants in water and sediments can be carcinogenic to aquatic wildlife as well as humans. Identifying those carcinogens, however, is difficult because they often occur in low concentrations and exert their effects over a large part of the life span of affected organisms. Furthermore, the carcinogens are often components of complex mixtures. Recent studies suggest that laboratory-reared fish species might be well suited for testing water-associated and other carcinogens. Here, we review the principal carcinogen exposure methods that utilize small fish species or can be adapted to utilize small fish species to detect carcinogens in aqueous environments. Emphasis is placed on methods for which the end-point is tumor induction. The methods discussed are dietary exposures, skin painting, embryo microinjection, early life stage (pulse) exposures, static water exposures, flow-through exposures, and controlled field exposures. Early life stage exposures seem to have the greatest utility with regard to carcinogen sensitivity, ease of administration, disposal of test compounds, and economy of materials and effort. For certain types of carcinogens, however, long-term flow-through exposures are probably required. In summary, small fish carcinogenesis models offer an array of methodologies that can be utilized in a variety of combinations depending on compounds tested, exposure parameters employed, and end points sought.     

Soil Remediation Techniques at Uncontrolled Hazardous Waste Sites

The A&WMA Critical Review entitled “Soil Remediation Techniques at Uncontrolled Hazardous Waste Sites” was presented by Ronald C. Sims, Department of Civil and Environmental Engineering, Utah State University, Logan, Utah. Dr. Sims presented his review at the 83rd Air & Waste Management Association Annual Meeting, held in Pittsburgh, Pennsylvania in June 1990. Prepared discussions presented during the Critical Review session are published here, along with some closing remarks by Dr. Sims. Ronald Harkov, Chairman of the Critical Review Subcommittee of the Publications Committee, served as moderator of the 1990 A&WMA Critical Review session.     

Characterizing natural degradation of tetrachloroethene (PCE) using a multidisciplinary approach

A site in mid-western Sweden contaminated with chlorinated solvents originating from a previous dry cleaning facility, was investigated using conventional groundwater analysis combined with compound-specific isotope data of carbon, microbial DNA analysis, and geoelectrical tomography techniques. We show the value of this multidisciplinary approach, as the different results supported each interpretation, and show where natural degradation occurs at the site. The zone where natural degradation occurred was identified in the transition between two geological units, where the change in hydraulic conductivity may have facilitated biofilm formation and microbial activity. This observation was confirmed by all methods and the examination of the impact of geological conditions on the biotransformation process was facilitated by the unique combination of the applied methods. There is thus significant benefit from deploying an extended array of methods for these investigations, with the potential to reduce costs involved in remediation of contaminated sediment and groundwater.Electronic supplementary materialThe online version of this article (10.1007/s13280-020-01418-5) contains supplementary material, which is available to authorized users.