Immediate biomarker responses to benzo[a]pyrene in polluted and unpolluted populations of the blue mussel (Mytilus edulis L.) at high-latitudes

Immediate biomarker responses of two high-latitude populations of the blue mussel Mytilus edulis to benzo[a]pyrene (B[a]P) were evaluated. Mussels collected from a clean and a polluted site in southwest Iceland were exposed to the nominal dose of 100 microg B[a]P L(-l) for 3 h, after 4 days of acclimatization in clean seawater. To test the sensitivity to the toxicant and immediate biological responses, the following biomarkers were used: DNA single strand breaks, heart rate and feeding rate. All the biomarkers revealed differences between the study sites. Irrespective of the origin of the organisms, the short time exposure to the high B[a]P concentration did not induce DNA single strand breaks or significantly affect the feeding rate. However, the heart rate results showed significantly different responses. The mussels from the polluted site (Reykjavík harbour) increased their heart rate when exposed to B[a]P, while no difference was observed between the heart rate values of the individuals from the clean site (Hvassahraun). The mussels seem to sense the pollutant they have been previously exposed to, and their acute response indicates physiological adaptation to the polluted environment. The results indicate limited sensitivity and temporal predictivity, i.e. transient measurable changes of these biomarkers, as well as showing that the background of the organisms should be considered when evaluating short-term biomarker responses to contaminants.     

Effects of elevated yolk testosterone levels on survival, growth and immunity of male and female yellow-legged gull chicks

Androgen hormones of maternal origin contained in the eggs of avian species are considered to have positive effects on offspring characteristics and performance. However, negative consequences have also been reported, suggesting that mothers may experience a trade-off between beneficial and detrimental effects of egg androgens to offspring fitness. We studied the effects of elevated yolk testosterone (T) concentration on survival, development and phenotype of male and female yellow-legged gull (Larus michahellis) chicks by injecting egg yolks with physiological doses of the hormone. Elevated yolk T resulted in a male-biased post-hatching sex ratio, T-treated clutches producing a greater proportion of males compared to control ones at day 4 post-hatching, likely resulting from a reduction of female embryonic survival, whereas no effect of hormone treatment on hatching success or short-term chick survival was observed. In addition, T depressed post-hatching body mass in both sexes but had no effects on the intensity of the cell-mediated immune response or skeletal growth. No sex differences in egg characteristics or chick phenotype were detected. Time to hatching was not affected by T, but females originating from first laid eggs hatched earlier than males of the same laying order, independently of hormone treatment. However, the implications of sex differences in hatching times are unclear in the study species. Taken together, our results suggest that female yellow-legged gulls may be constrained in transferring androgens to their eggs by negative consequences on the viability of female offspring and growth of chicks of the two sexes.     

Study of atmospheric ozone formation by means of a neural network-based model

The occurrence of high ozone levels in the atmosphere of urban areas has become a serious pollution problem in a number of large cities in the world. Although mathematical models have been proposed for predicting ozone concentrations as a function of a number of gas components, sometimes there are uncertainties due to lack of the combined effects of meteorological factors and the complex chemical reaction system involved. The application of neural network models, based on measured values of air pollutants and meteorological factors at different locations within the S?o Paulo Metropolitan Area, combine chemical and meteorological information. This has shown to be a promising tool for predicting ozone concentration. Simulations carried out with the model indicate the sensitivity of ozone in relation to different air pollution and weather conditions. Predictions using this model have shown good agreement with measured values of ozone concentrations.     

Water-soluble extracts of the diatom Thalassiosira rotula induce aberrations in embryonic tubulin organisation of the sea urchin Paracentrotus lividus

Eggs and embryos of the sea urchin Paracentrotus lividus were used as a model to study the effect at the cellular level of potential anti-mitotic compounds extracted from the diatom Thalassiosira rotula. Eggs and embryos incubated in a water-soluble diatom extract, corresponding to 5 × 10⁶ and 10⁷ cells ml⁻¹, were totally blocked (i.e. cell division was blocked) at the one-cell stage. At lower concentrations (2.5 and 1.25 × 10⁶ cells ml⁻¹), the first mitotic division was inhibited in 32 ± 26% and 25 ± 3.5% of the zygotes, respectively, demonstrating the dose-dependent effect of diatom extracts on sea urchin development. Immunofluorescence dyes, specific for DNA and α-tubulin subunits, were used to stain nuclei and microtubules in sea urchin embryos during various phases of development. Images with the confocal laser scanning microscope showed that tubulin was not organised in filaments at the sperm aster and cortex levels, and that the pronuclei were not fused in embryos incubated soon after fertilisation with water-soluble diatom extracts corresponding to 10⁷ cells ml⁻¹. At lower diatom-extract concentrations (4 × 10⁶ cells ml ⁻¹), fusion of the pronuclei occurred but the mitotic spindle was not formed. Microtubules were clearly de-polymerised and the chromatin appeared globular and compacted at the centre of the cell. A similar structure was observed for sea urchin embryos incubated with 0.1 mM colchicine, a potent anti-mitotic compound. When sea urchin embryos were incubated in water-soluble diatom extracts at different times prior to the first mitotic division, microtubules appeared de-polymerised at each step, from pronuclear fusion to telophase, and cell division was blocked. At the histological level, embryos incubated with 4 × 10⁶ cells ml⁻¹ diatom extract showed nuclear fragmentation without cytokinesis. The possible use of sea urchin embryos as a bioassay to test for other unknown compounds with cytotoxic activity in phytoplankton species is discussed. Received: 7 May 1998 / Accepted: 9 December 1998     

Atmospheric mercury at mediterranean coastal stations

Mercury in air has been measured at five coastal Mediterranean sites, involving measurements in Spain, France, Italy, Slovenia and Israel. Four two-weeks long measurements campaigns were performed at the five sites. The measurements were carried out during autumn 2003 and winter, spring and summer 2004. Total gaseous mercury/elemental gaseous mercury, particulate mercury and divalent gaseous mercury were measured in parallel at the five sites. The activities constituted a subtask of the EU funded MERCYMS research project, which also included Mediterranean Sea cruises where both mercury in air and water were measured. The result from an evaluation of all the coastal air data is presented. Mercury concentrations from the different sites are compared with similar data obtained in northern Europe and elsewhere. The result shows that the background concentration of mercury in Mediterranean coastal air is lower than earlier anticipated. Background concentrations of TGM, RGM and TPM corresponded to 1.75–1.80 ng m⁻³, 1–13 and 3–23 pg m⁻³, respectively. The measurements also showed that the mercury concentration occasionally can be very high in some areas due to local anthropogenic emissions. It is proposed that diurnal variation in RGM concentrations observed during situation with nocturnal inversion merely is an effect of meteorology rather than due to local photochemistry.     

Within-clutch egg size asymmetry covaries with embryo sex in the yellow-legged gull Larus michahellis

Non-random sex allocation may occur whenever the expected reproductive value of sons and daughters differs, as is the case when the sexes differ in susceptibility to environmental conditions or maternal effects (e.g. egg size and hatch order). Under such circumstances, covariation between egg and clutch characteristics and egg sex may be expected, and this covariation should vary with maternal state or ecological conditions. In this 2-year study (2007–2008), we examined sex allocation in relation to egg and clutch traits in the yellow-legged gull Larus michahellis, a species where male chicks are larger and more susceptible to harsh rearing conditions than female ones. In 2008, eggs were more likely male early in the season in two- but not three-egg clutches, and large eggs were more likely males late in the season. No egg/clutch traits predicted egg sex in 2007. Within-clutch egg mass asymmetry (the difference in egg mass between the first- and last-laid eggs) predicted sex in both years. In 2007, clutches with smaller egg mass variation were more likely to contain males, while in 2008 this relationship held for the last-laid egg and was reversed for the preceding egg(s). Laying order and sex of the previous egg did not predict egg sex, providing no evidence of sex-specific oocyte clustering. Thus, the relationships between egg sex and egg/clutch traits differed among years, suggesting a phenotypically plastic response of females to extrinsic conditions, and involved within-clutch egg mass asymmetry, a trait likely reflecting variation in maternal quality and/or reproductive tactics, which has been largely neglected in previous studies of sex allocation.     

Parent-absent signalling of need and its consequences for sibling competition in the barn swallow

In species with parental care, competition among siblings for access to limited parental resources is common. Sibling competition can be mediated by begging behaviour, a suite of different visual and acoustic displays by which offspring solicit parental care. These are mostly addressed to the parents upon food provisioning, but can also be performed in the absence of the attending parents. This so-called parent-absent begging (PAB) may function as an intrabrood communication signal and potentially affect intrabrood competition dynamics for access to food. We investigated the role of PAB in moulding sibling interactions and its effect on food intake among altricial barn swallow (Hirundo rustica) nestlings, both under normal and experimentally reduced food intake. Frequency of PAB increased after food deprivation. Nestlings that had performed PAB increased their begging intensity upon the subsequent parental feeding visit, while siblings reduced their own begging level, but only when they had not been food-deprived. As a consequence, nestlings which had performed PAB before parental arrival had larger chances of receiving food. However, nestlings did not benefit from displaying PAB when competing with food-deprived siblings. Our findings show that PAB reliably reflects need of food, indicating that a nestling will vigorously compete for the subsequent food item. By eavesdropping siblings' PAB displays, nestlings may optimally balance the costs of scrambling competition, the direct fitness gains of being fed and the indirect fitness costs of subtracting food to needy kin. However, large asymmetries in satiation between competitors may lead individual offspring to monopolize parental resources, irrespective of PAB displays.     

Study of fluxing in Al refining process by rotary and crucible furnaces

The process used to obtain recycled Al alloys depends on the scrap quality. Rotary furnaces are normally used in Europe as they allow a great productivity and they can process low quality scrap using a salt flux to protect the molten metal from oxidation. In this article, the effect of the quantity of salt on the metal recovery has been experimentally investigated by melting several types of scrap under a mixture of NaCl–KCl–Na₃AlF₆ salts. The quantity of salt has been related to the salt factor (SF), which is the ratio between the non-metallic content in the scrap and the quantity of salt required. The same levels of salt factor have been tested by melting the scrap in rotary and crucible furnaces. The metal recovery increases with the salt content for both furnaces. The results obtained with the different melting processes are comparable because the yield difference is similar considering different salt factors. Crucible furnace gives greater metal recovery but it cannot replace rotary furnace in Al refining industry due to lower melting capacity. However, the results highlight that the optimisation of the Al refining process can be supported by preliminary tests through crucible furnaces reducing the time and material consumptions.     

Length of index and ring fingers differentially influence sexual attractiveness of men’s and women’s hands

Humans show intra- and intersexual variation in second (2D) relative to fourth (4D) finger length, men having smaller 2D:4D ratio, possibly because of differential exposure to sex hormones during fetal life. The relations between 2D:4D and phenotypic traits including fitness components reported by several studies may originate from the organizational effects that sex hormones have on diverse organs and their concomitant effect on 2D:4D. Evolutionary theory posits that sexual preferences are adaptations whereby choosy individuals obtain direct or genetic indirect benefits by choosing a particular mate. Since sex hormones influence both fitness and 2D:4D, hand sexual attractiveness should depend on 2D:4D, a hypothesis tested only in one correlational study so far. We first presented hand computer images to undergraduates and found that opposite-sex hands with long 2D and 4D were considered more sexually attractive. When we experimentally manipulated hand images by increasing or decreasing 2D and/or 4D length, women preferred opposite-sex hands that had been masculinized by elongating 4D, whereas men avoided masculinized opposite-sex right hands with shortened 2D. Hence, consensus exists about which hands are attractive among different opposite-sex judges. Finger length may signal desirable sex hormone-dependent traits or genetic quality of potential mates. Psychological mechanisms mediating hand attractiveness judgments may thus reflect adaptations functioning to provide direct or indirect benefits to choosy individuals. Because the genetic mechanisms that link digit development to sex hormones may be mediated by Hox genes which are conserved in vertebrates, present results have broad implications for sexual selection studies also in nonhuman taxa.     

Incipient Forest Decline in the Province of Trento (Northern Italy). Preliminary Sem Observations and Consideration of Inorganic Components of Leaves and Roots

One year old needles of both healthy and damaged silver fir and Norway spruce from the area of Trento (Northern Italy) were observed by SEM. Needles of damaged trees show quite evident structural alterations of epidermis, expecially epicuticular waxes. Roots and needles of both fir and spruce are also tested for their metal content. Evidence is reported of an increasing concentration of Fe, Mg and Ca in roots of plants grown on alkali soil. Ca, and to a lesser extent Fe, Al and Pb, appears to be more present in old needles, whereas K and Mg exhibit higher amounts in young needles. Some indications are found between damage and the content of some metals such as Mg and Mn in needles.