Temporal and geographical genetic variation in the amphipod Melita plumulosa (Crustacea: Melitidae): Link of a localized change in haplotype frequencies to a chemical spill

Anthropogenic effects such as contamination affect the genetic structure of populations. This study examined the temporal and geographical patterns of genetic diversity among populations of the benthic crustacean amphipod Melita plumulosa in the Parramatta River (Sydney, Australia), following an industrial chemical spill. The spill of an acrylate/methacrylate co-polymer in naphtha solvent occurred in July 2006. M. plumulosa were sampled temporally between December 2006 and November 2009 and spatially in November 2009. Genetic variation was examined at the mitochondrial cytochrome c oxidase subunit I locus. Notably, nucleotide diversity was low and Tajima’s D was significantly negative amongst amphipods collected immediately downstream from the spill for 10 months. We hypothesize that the spill had a significant localized effect on the genetic diversity of M. plumulosa. Alternate explanations include an alternate and unknown toxicant or a localized sampling bias. Future proposed studies will dissect these alternatives.     

Developmental toxicity of two common corn pesticides to the endangered southern bell frog (Litoria raniformis)

To examine the link between corn agriculture and the observed decline of the endangered southern bell frog (SBF), the effects of two corn crop pesticides on larval growth and development were investigated. Tadpoles were exposed to terbufos sulfone (10 μg/L), a major breakdown product of the insecticide terbufos, and the herbicide atrazine (25 μg/L) individually and as a mixture until the completion of metamorphosis. Atrazine did not interact synergistically with terbufos sulfone or result in significant effects on growth and development alone, although there was some indication of accelerated metamorphosis in the pilot study. Terbufos sulfone alone and as a mixture (terbufos/atrazine) significantly slowed larval development and ultimately delayed metamorphosis. The observed developmental effects from an environmentally relevant concentration of terbufos sulfone indicates a risk posed by this persistent degradation product to the endangered SBF, which breeds and develops in the rice bays adjacent to corn fields treated with pesticides.     

Green roofs as a means of pollution abatement

Green roofs involve growing vegetation on rooftops and are one tool that can help mitigate the negative effects of pollution. This review encompasses published research to date on how green roofs can help mitigate pollution, how green roof materials influence the magnitude of these benefits, and suggests future research directions. The discussion concentrates on how green roofs influence air pollution, carbon dioxide emissions, carbon sequestration, longevity of roofing membranes that result in fewer roofing materials in landfills, water quality of stormwater runoff, and noise pollution. Suggestions for future directions for research include plant selection, development of improved growing substrates, urban rooftop agriculture, water quality of runoff, supplemental irrigation, the use of grey water, air pollution, carbon sequestration, effects on human health, combining green roofs with complementary related technologies, and economics and policy issues.     

Metal contents of marine turtle eggs (Chelonia mydas; Lepidochelys olivacea) from the tropical eastern pacific and the implications for human health

Concentrations of eight elements were measured in Chelonia mydas and Lepidochelys olivacea eggs collected along the Pacific coast of Panama. Manganese (Mn), iron (Fe), copper (Cu), zinc (Zn), arsenic (As), cadmium (Cd), and mercury (Hg) concentrations were similar to previous reports of these species from around the world, while lead (Pb) was lower than previous reports. Cd posed the highest health risk to people who regularly eat the eggs, with average consumption rates leading to target hazard quotients (THQ) of up to 0.35 ± 0.15. Our conclusions indicate that current turtle egg consumption in isolated, coastal Pacific communities may pose a health concern for young children, and that youth and young adults should limit their consumption of turtle eggs to reduce their total intake of nonessential metals.     

Extraction of labile metals from solid media by dilute hydrochloric acid

Extraction of labile metals from solid media is environmentally more meaningful than a total digestion. A variety of reagents have been introduced in the literature, but dilute HCl has received the greatest attention. We compare metal concentrations liberated by a dilute HCl leach with the sum of the 3-step optimized (standardized) BCR sequential extraction procedure. This is the first study to compare these procedures over a range of grain sizes. Road-deposited sediments from 10 sites in Honolulu were fractionated into six grain size classes. Aliquots of individual fractions were digested with dilute HCl, the 3-step BCR procedure (‘labile’), and a 4-acid (total) procedure. Results indicated that the weighted labile concentrations of Al, Cu, Fe, Mn, Ni, Pb and Zn were statistically greater than those from the dilute HCl leach. However, regression analysis indicated strong statistically significant relationships between the two partial extraction procedures for all metals. On a whole-sample basis, the toxicity classifications for anthropogenic-enhanced metals (Cu, Pb and Zn) were similar between extractions. Taken together, results suggest that the application of dilute HCl to solid media provides a rapid, cost-effective, and environmentally meaningful approach for contaminant monitoring.     

Nitrogen deposition increases the acquisition of phosphorus and potassium by heather Calluna vulgaris

Increased plant productivity due to nitrogen pollution increases the strength of the global carbon sink, but is implicated in plant diversity loss. However, modelling and experimental studies have suggested that these effects are constrained by availability of other nutrients. In a survey of element concentrations in Calluna vulgaris across an N deposition gradient in the UK, shoot concentrations of N and more surprisingly phosphorus and potassium were positively correlated with N deposition; tissue N/P ratio even decreased with N deposition. Elevated P and K concentrations possibly resulted from improved acquisition due to additional enzyme production or mycorrhizal activity. Heather occurs on organic soils where nutrient limitations are likely due to availability constraints rather than small stocks. However, if this effect extends to other plant and soil types, effects of N deposition on C sinks and plant competition may not be as constrained by availability of other nutrients as previously proposed.     

Laboratory and field estimates of the rate of faecal pellet production by Antarctic krill,Euphausia superba

The time course of faecal pellet production (egestion) was monitored in January 1985 for a population of Antarctic krill, Euphausia superba Dana, maintained in flowing seawater aquaria at Palmer Station, Antarctica. Following transfer to filtered seawater, krill produced faecal strings for roughly 40 min, after which time faecal egestion virtually ceased. Similar results were obtained for freshly-trawled krill at sea in February and March 1985. There were wide daily variations in total faecal egestion rate; mean rates varied from 0.54 to 1.66 mg dry wt h^-1 and individual rates from 0.25 to 2.35 mg h^-1 (all data corrected to a standard krill of 600 mg fresh weight). Despite these wide fluctuations in total faecal egestion, the loss of organic matter showed no significant daily variation, with a mean value of 0.13 mgh^-1. The relationship between faecal egestion rate and faecal organic content suggested that feeding rate was governed by food quality; when inorganic load was high, feeding rate increased to ensure sufficient energy intake. The data suggest that superfluous feeding does not occur in krill and that values of gut-clearance time calculated from time intervals greater than about 40 min will not be representative of previous feeding history. the rates of faecal egestion observed in this study indicate that the flux of faecal pellets from krill is substantial. They imply an energy intake in E. superba of 17 to 28% body weight per day, much higher than estimated previously for this species by summing known energy losses, but similar to estimates for other euphausiids.     

Modelling leachate quality and quantity in municipal solid waste landfills.

The operational phase of landfills may last for 20 years or more. Significant changes in leachate quality and generation rate may occur during this operational period. A mathematical model has been developed to simulate the landfill leachate behaviour and distributions of moisture and leachate constituents through the landfill, taking into consideration the effects of time-dependent landfill development on the hydraulic characteristics of waste and composition of leachate. The model incorporates governing equations that describe processes influencing the leachate production and biochemical processes taking place during the stabilization of wastes, including leachate flow, dissolution, acidogenesis and methanogenesis. To model the hydraulic property changes occurring during the development stage of the landfills, a conceptual modelling approach was proposed. This approach considers the landfill to consist of cells or columns of cells, which are constructed at different times, and considers each cell in the landfill to consist of several layers. Each layer is assumed to be a completely mixed reactor containing uniformly distributed solid waste, moisture, gases and micro-organisms. The use of the proposed conceptual model enables the incorporation of the spatial changes in hydraulic properties of the landfill into the model and also makes it possible to predict the spatial and temporal distributions of moisture and leachate constituents. The model was calibrated and partially verified using leachate data from Keele Valley Landfill in Ontario, Canada and data obtained from the literature. Ranges of values were proposed for model parameters applicable for real landfill conditions.