First-trimester prenatal diagnosis of cystic fibrosis using fibroblasts from a deceased index child to establish haplotypes

First-trimester prenatal diagnosis of cystic fibrosis (CF) using linked DNA markers is usually only possible if there is an index affected child to establish the haplotype of the parental chromosomes. We describe a prenatal diagnosis where fibroblasts, cultured from the skin of a deceased affected child and then held in frozen storage for 3 years, were used as the starting point for tracking the CF gene. The fetus was diagnosed as a homozygous normal and the diagnosis confirmed by immunoreactive trypsin testing after birth. It was also possible to establish heterozygosity in the aunt of the affected child.     

Nonindigenous biota on artificial structures: could habitat creation facilitate biological invasions?

We identified different distributions of marine nonindigenous species (NIS) and native species on some artificial structures versus natural reefs and using experimental manipulations, revealed some possible causal mechanisms. In well-established subtidal assemblages, numbers of NIS were 1.5–2.5 times greater on pontoons or pilings than on rocky reefs, despite the local species pool of natives being up to 2.5 times greater than that of NIS. Conversely, on reefs and seawalls, numbers of native species were up to three times greater than numbers of NIS. Differential recruitment to different positions and types of surfaces appeared to influence distribution patterns. NIS recruited well to most surfaces, particularly concrete surfaces near the surface of the water, whilst natives occurred infrequently on wooden surfaces. The position of rocky reefs and seawalls close to the shore and to the seabed appeared to make them favourable for the recruitment of natives, but this positioning alone does not hinder the recruitment of NIS. We argue that pontoons and pilings represent beachheads (i.e. entry points for invasion) for many nonindigenous epibiota and so enhance the spread and establishment of NIS in estuaries. Habitat creation in estuaries may, therefore, be a serious threat to native biodiversity. Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users.     

Garden sharing and garden stealing in fungus-growing ants

 Fungi cultivated by fungus-growing ants (Attini: Formicidae) are passed on between generations by transfer from maternal to offspring nest (vertical transmission within ant species). However, recent phylogenetic analyses revealed that cultivars are occasionally also transferred between attine species. The reasons for such lateral cultivar transfers are unknown. To investigate whether garden loss may induce ants to obtain a replacement cultivar from a neighboring colony (lateral cultivar transfer), pairs of queenright colonies of two Cyphomyrmex species were set up in two conjoined chambers; the garden of one colony was then removed to simulate the total crop loss that occurs naturally when pathogens devastate gardens. Garden-deprived colonies regained cultivars through one of three mechanisms: joining of a neighboring colony and cooperation in a common garden; stealing of a neighbor's garden; or aggressive usurpation of a neighbor's garden. Because pathogens frequently devastate attine gardens under natural conditions, garden joining, stealing and usurpation emerge as critical behavioral adaptations to survive garden catastrophes. Received: 16 June 2000 / Accepted in revised form: 14 September 2000     

Environmental impact assessment in ghana-anex post evaluation of the volta resettlement scheme: the case of the Kpong Hydro-electric project

Summary Many Third World Countries (TWCs), like Ghana, have not concerned themselves seriously with environmental problems as part of the entire socio-economic development process, until recently. This, for example, can be seen in their environmental policies and the rate at which these now are being made. Not only are environmental issues being considered in the formulation and implementation of new development programmes and projects, but it is being realised that earlier implemented projects need some evaluation,ex post, for possible, correction of past mistakes or, at least, as a source of self-containment. Thus, there has been interest in the impact assessment of past macro-projects as agricultural and resettlement schemes.An attempt is made to apply a simple matrices methodology to theex post evaluation of the Kpong Hydro-electric Project resettlement aspect, based on identified project and environment components. The results show that the impact on the socio- economic life of the settlers is positively greater than the physical and biological impacts. But considering other elements of the programme package and taking a perspective view, the settlers are after all, no better off. In view of the fact that a number of such schemes are now under consideration, it is useful for policy-makers and planners to learn from these lessons.Sam Ofori-Cudjoe is a Lecturer in Geography at the University of Cape Coast, Ghana. He is currently studying planning research at the Centre for Planning, University of Strathclyde, Glasgow. He obtained a BA (Hons) in Geography from the Department of Geography, University of Ghana, Legon, in 1978, and a MSc (Regional Planning) from the Department of Planning, University of Science and Technology (UST), Kumasi, Ghana, in 1981.     

MONITORING OPERATIONS PERFORMANCE IN LARGE-SCALE PUBLIC IRRIGATION SYSTEMS IN INDONESIA1

ABSTRACT: Different allocation and delivery performance ratios are used to assess operations performance in a large scale public irrigation system in Indonesia. Results from this analysis indicate that field management practices deviate substantially from the official operating procedures. The lack of application of a single, standardized procedure for planning water allocations represents a serious constraint to effective monitoring and evaluating system performance. Underestimated and unrealistic planned allocations becomes the justification for overdiversion of water, and has the effect of undermining the resolve of managers to see to it that actual flows meet planned flows. Miscalculating planned allocations, poor matches between planned flows and actual deliveries, overdiversion and misreporting have economic consequences as well. For example, strict adherence to the standard operating rule during the second dry season of 1987 would have resulted in 19,070,000 m³ less water diverted into the irrigation system from the Brantas River or, at a conservative estimate of $1.78 per 100 m³ water, a savings of $339,500. Scope exists for making improvements in management by closer adherence to the standard delivery rule, which will also facilitate proper monitoring and evaluation.     

Towards improved socio-economic assessments of ocean acidification’s impacts

Ocean acidification is increasingly recognized as a component of global change that could have a wide range of impacts on marine organisms, the ecosystems they live in, and the goods and services they provide humankind. Assessment of these potential socio-economic impacts requires integrated efforts between biologists, chemists, oceanographers, economists and social scientists. But because ocean acidification is a new research area, significant knowledge gaps are preventing economists from estimating its welfare impacts. For instance, economic data on the impact of ocean acidification on significant markets such as fisheries, aquaculture and tourism are very limited (if not non-existent), and non-market valuation studies on this topic are not yet available. Our paper summarizes the current understanding of future OA impacts and sets out what further information is required for economists to assess socio-economic impacts of ocean acidification. Our aim is to provide clear directions for multidisciplinary collaborative research.     

Management of water resources of the Nile Basin

SUMMARY

A water crisis is looming in the Nile Basin in the near future due to higher population growth rates, greater affluence, and higher demand for water by agriculture and industry. There is no basin-wide agreement among the ten Nile Basin states and the existing rules of international water management are complicated and are not equipped to handle any future water conflicts in the Nile Basin. This paper reviews the existing water sharing arrangements among the Nile Basin countries, examines areas of potential conflict over future water use, and suggests possible areas of cooperation that will ensure equitable use of the Nile waters. The paper concludes by (1) appealing for formulation of good water development policies to deal with growing water needs such as water quality protection, efficiency of water delivery and efficiency of water use; and (2) calling for human resources development and technology transfers that are critical for good management and efficient use of the water resources of the Nile.     

Toward an inherently safer design and operation of batch and semi-batch processes: The N-oxidation of alkylpyridines

This work shows an application of inherent safety principles to a reaction widely used in the pharmaceutical industry. More specifically, it incorporates the teachings of Trevor Kletz into the design of an inherently safer process for the N-oxidation of alkylpyridines. This reaction is of interest because of the hazards resulting from the undesired, gas-generating decomposition of hydrogen peroxide, the oxidizing agent. The generation of oxygen, combined with the flammability of the alkylpyridines, represents a serious fire and explosion hazard for this process. The purpose of this paper is to demonstrate how an inherently safer process can be potentially achieved by designing improved reactors and by assessing conditions that reduce or eliminate the hazards. Furthermore, it is shown that such improvement in safety increases the efficiency of the process and results in a cost reduction.     

Calibrate failure-based risk assessments to take into account the type of chemical processed in equipment

Currently, failure-based risk assessments in the process industry do not empirically take into account the type of chemicals processed in equipment, mainly because chemical-specific failure rate data barely exist. This paper suggests a methodology to calibrate failure-based risk assessment predicated on the chemical being processed in equipment. The methodology uses a data mining tool known as the association rule. Specifically, the lift association rule is utilized (the Lift Methodology). By extracting equipment failure information from incident databases based on the chemical involved in the process, the Lift Methodology leads to more accurate equipment-related risk assessment.     

Effects of crop diversification and low pesticide inputs on bird populations on arable land

This 6-year experimental study measured the response of bird populations and abundance to combinations of mixed cropping and low pesticide regimes associated with a commercial crop rotation. The results show a rapid and sustained population increase among a wide range of bird species, in contrast to local regional trends for the same species. Seventy percent of the increase occurred within the first 3 years of the experiment, with species of high conservation concern, and those monitored as environmental indicators on lowland farmland in the UK, increasing on average, by 30% and 20% respectively (reaching respective peaks of 44% and 33% after 4 years). For some individual species, the increase was higher still, i.e., 300% (1–4 pairs) for grey partridge (Perdix perdix) and 46% (13–19 pairs) for skylarks (Alauda arvensis) in peak years. The results demonstrate that bird species typical of lowland arable farmland in the UK are responsive to suitable farm-scale changes in habitat and food provision (roughly, manipulation within less than 1-km²). They show that the carrying capacity of modern, commercially viable, arable farmland can be increased significantly for birds, in this case, mainly by using crops mosaics to create habitats alongside the appropriate use of herbicides on non-cropped habitats.