Identifying trophic variation in a marine suspension feeder: DNA- and stable isotope-based dietary analysis in Mytilus spp.

Accurate field data on trophic interactions for suspension feeders are lacking, and new approaches to dietary analysis are necessary. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) was integrated with stable isotope analysis to examine dietary patterns in suspension-feeding Mytilus spp. from seven spatially discrete locations within a semi-enclosed marine bay (Strangford Lough, Northern Ireland) during June 2009. Results of the two methods were highly correlated, reflecting dietary variation in a similar manner. Variation in PCR-DGGE data was more strongly correlated with the principal environmental gradient (distance from the opening to the Irish Sea), while values of δ ¹³C and δ ¹⁵N became progressively enriched, suggesting a greater dependence on animal tissue and benthic microalgae. Diatoms and crustaceans were the most frequently observed phylotypes identified by sequencing, but specific DNA results provided little support for the trophic trends observed in the stable isotope data. This combined approach offers an increased level of trophic insight for suspension feeders and could be applied to other organisms.     

Mechanisms of habitat segregation between an invasive (Mytilus galloprovincialis) and an indigenous (Perna perna) mussel: adult growth and mortality

The invasive mussel Mytilus galloprovincialis and the indigenous mussel Perna perna coexist intertidally on the south coast of South Africa through partial vertical habitat segregation: M. galloprovincialis dominates the upper shore and P. perna the lower shore. Recruitment patterns can explain the zonation of P. perna, but not the invasive species. We examined the role of post-recruitment interactions by measuring spatial and temporal differences in adult growth and mortality rates of the two species. Specifically, we tested the hypothesis that interspecific differences in growth and mortality reflect adult distribution patterns. The two study locations, Plettenberg Bay and Tsitsikamma, are 70 km apart with two sites (separated by 300–400 m) per location, each divided into three vertical zones. Growth was measured seasonally using different marking methods in 2001 and 2003. Cumulative adult mortality was measured through summer in 2003/2004. Both species generally grew more slowly upshore, but they showed different effects of season. For P. perna, growth was significantly reduced in winter in the low zone, but unaffected by season in the high zone. For M. galloprovincialis, growth was either unaffected by season or increased in winter, even in the high zone. Thus, growth of P. perna and M. galloprovincialis was reduced under cool winter and warm summer temperatures, respectively; and while growth was more similar between species in summer, M. galloprovincialis grew much faster than P. perna in winter. Mortality of P. perna increased upshore. For M. galloprovincialis, mortality was not zone-dependent and was significantly greater than for P. perna on the low-shore and (generally) across the shore in Tsitsikamma. Both species had higher growth and mortality rates in Plettenberg Bay than in Tsitsikamma. Thus, P. perna seems able to maintain spatial dominance on the low-shore and at certain sites because of higher mortality of M. galloprovincialis. We conclude that seasonality in growth of the two species reflects their biogeographic affinities and that coexistence is possible through pre-recruitment effects that limit the vertical distribution of P. perna and post-recruitment effects that limit M. galloprovincialis.     

Production of food-associated calls in wild male chimpanzees is dependent on the composition of the audience

Chimpanzees produce acoustically distinct calls when encountering food. Previous research on a number of species has indicated that food-associated calls are relatively widespread in animal communication, and the production of these calls can be influenced by both ecological and social factors. Here, we investigate the factors influencing the production of food-associated calls in wild chimpanzees and examine whether male chimpanzees produce food-associated calls selectively in the presence of important social partners. Male chimpanzees form stable long-term social relationships with each other, and these social bonds are vital in enabling a range of cooperative activities, such as group hunting and territory defence. Our data show that males were significantly more likely to produce food-associated calls if an important social partner was nearby, regardless of the size of the audience or the presence of oestrus females. Call production was also mediated by the size of the food patch and by whether or not the food could be monopolised. The presence of important social partners explained most of the variation in male calling behaviour, indicating that food-associated calls are socially directed and serve a bonding function.     

Preliminary Caesium Data From A Cooperative Us/Ussr Monitoring Survey for Chernobyl Radioactivity in the Black Sea

In June 1990, scientists from the US Environmental Protection Agency's (EPA) Office of Radiation Programs (ORP), and the Woods Hole Oceanographic Institution (WHOI), travelled to Sevastopol in the Soviet Union to work with radioecologists and marine scientists from the USSR Institute of Biology of the Southern Seas (IBSS). the purpose of this cooperative programme was to conduct a monitoring survey for radioactivity in the northwestern Black Sea. Samples of sediment, surface and in-situ water, and biota were collected from fourteen stations for post-survey radionuclide analyses to determine levels of radioactivity in the Black Sea environment resulting from the Chernobyl nuclear power plant explosion and subsequent transport of radioactivity via the Dnepr and Danube rivers. This paper presents the preliminary data for caesium-137 and caesium-134 in sediment samples analyzed by the EPA/ORP. Caesium-137 was measured at four shallow (20-114 m) stations on the shelf near the mouth of the Dnepr and Danube Rivers, but was not detected in sediments from comparable depths at stations further off shore or in slope sediments at depths of 510-1288 meters. Caesium-134 was detected only in sediments from the shallow-water station nearest to the Danube River.     

Human exposure to 2,3,7,8-TCDD

This paper uses an environmental partitioning model to evaluate the concentration of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) in various environmental media. These concentrations are then used to estimate the amount of TCDD entering the food chain and the average daily intake of TCDD by the general population of the US. The food chain accounts for 98% of human exposure to TCDD. The model estimated the average daily intake of TCDD to be 0.05 ng/day.     

Integrating Bioassessment and Ecological Risk Assessment: An Approach to Developing Numerical Water-Quality Criteria

Bioassessment is used worldwide to monitor aquatic health but is infrequently used with risk-assessment objectives, such as supporting the development of defensible, numerical water-quality criteria. To this end, we present a generalized approach for detecting potential ecological thresholds using assemblage-level attributes and a multimetric index (Index of Biological Integrity—IBI) as endpoints in response to numerical changes in water quality. To illustrate the approach, we used existing macroinvertebrate and surface-water total phosphorus (TP) datasets from an observed P gradient and a P-dosing experiment in wetlands of the south Florida coastal plain nutrient ecoregion. Ten assemblage attributes were identified as potential metrics using the observational data, and five were validated in the experiment. These five core metrics were subjected individually and as an aggregated Nutrient–IBI to nonparametric changepoint analysis (nCPA) to estimate cumulative probabilities of a threshold response to TP. Threshold responses were evident for all metrics and the IBI, and were repeatable through time. Results from the observed gradient indicated that a threshold was 50% probable between 12.6 and 19.4 g/L TP for individual metrics and 14.8 g/L TP for the IBI. Results from the P-dosing experiment revealed 50% probability of a response between 11.2 and 13.0 g/L TP for the metrics and 12.3 g/L TP for the IBI. Uncertainty analysis indicated a low (typically 5%) probability that an IBI threshold occurred at 10 g/L TP, while there was 95% certainty that the threshold was 17 g/L TP. The weight-of-evidence produced from these analyses implies that a TP concentration > 12–15 g/L is likely to cause degradation of macroinvertebrate assemblage structure and function, a reflection of biological integrity, in the study area. This finding may assist in the development of a numerical water-quality criterion for TP in this ecoregion, and illustrates the utility of bioassessment to environmental decision-making.     

Integrating spatially referenced social and biophysical data to explore landholder responses to dryland salinity in Australia

Researchers attempting to integrate socio-economic data in watershed planning often draw on nationally collected census data. However, there are critical limitations to the usefulness of this type of data for decision makers operating at the watershed scale. In this paper we demonstrate the relevance of spatially referenced socio-economic data collected using mail surveys to random selections of rural landholders. The issue explored was dryland salinity management in two large watersheds in the Murray-Darling Basin of south-eastern Australia. Contrary to the assumptions underlying public policy in Australia, but consistent with the literature on farmer knowledge, comparisons of expert maps and landholder identified salinity sites suggested that landholders in these watersheds had excellent knowledge of the current extent of salinity on their property. Our research also suggested that salinity education was a sound investment by governments. At the same time, the expert maps failed to predict half of the saline affected sites identified by landholders. Accurately mapping the extent of salinity would seem a first step in addressing this nationally significant land degradation issue.     

Watershed associations in West Virginia: their impact on environmental protection

Grassroots watershed associations have formed as an avenue to facilitate public involvement in protecting watersheds. Growth in the number of watershed associations has created a need to evaluate whether their existence enhances environmental protection by local communities. In this research, environmental protection was measured by the number of watershed protection activities conducted and amount of funding directed towards protecting surface waters by non-profit, volunteer organizations. Regression models were utilized to examine the influence of population demographic and watershed characteristics variables to explain the formation of watershed associations along with watershed protection activities and funding. Regression results showed that the presence of watershed associations had positive impacts on both the number of activities and financial resources acquired. The results confirm that watershed associations enhance the ability of communities to obtain funding and to implement watershed protection actions. Thus, watershed associations serve as an effective institution for promoting environmental protection.     

Resource problems in the eighties: An essay

Summary This article presents a brief overview of the more severe problems which must be addressed by natural resource planners and managers in the eighties.Professor Fred Curtis is senior author of this paper which, although written in a style which offers light reading, serves as a reminder from Canada that the resource problems of the '70s are ever present today in the '80s. The address given is that of Prof. Curtis. Paul Simpson-Housley is a member of staff in the Department of Geography, York University, 4700 Keele Street, Downsview, Ontario M3J 1P3 and Messrs Kent and Blakely maybe contacted through the senior author.     

Remote sensing for land management and planning

The primary role of remote sensing in land management and planning has been to provide information concerning the physical characteristics of the land which influence the management of individual land parcels or the allocation of lands to various uses These physical characteristics have typically been assessed through aerial photography, which is used to develop resource maps and to monitor changing environmental conditions These uses are well developed and currently well integrated into the planning infrastructure at local, state, and federal levels in the United States.Many newly emerging uses of remote sensing involve digital images which are collected, stored, and processed automatically by electromechanical scanning devices and electronic computers Some scanning devices operate from aircraft or spacecraft to scan ground scenes directly; others scan conventional aerial transparencies to yield digital images. Digital imagery offers the potential for computer-based automated map production, a process that can significantly increase the amount and timeliness of information available to land managers and planners.Future uses of remote sensing in land planning and management will involve geographic information systems, which store resource information in a geocoded format. Geographic information systems allow the automated integration of disparate types of resource data through various types of spatial models so that with accompanying sample ground data, information in the form of thematic maps and/ or aerially aggregated statistics can be produced Key issues confronting the development and integration of geographic information systems into planning pathways are restoration and rectification of digital images, automated techniques for combining both quantitative and qualitative types of data in information-extracting procedures, and the compatibility of alternative data storage modes