Global genomic methylation levels in the liver and gonads of the three-spine stickleback (Gasterosteus aculeatus) after exposure to hexabromocyclododecane and 17-beta oestradiol

Alteration of DNA methylation is a major epigenetic mechanism associated with the effects of nongenotoxic carcinogens. We evaluated the effects of two environmental pollutants, hexabromocyclododecane (HBCD), 17-beta oestradiol (E(2)) as well as 5-aza 2' deoxycytidine (5AdC) on global DNA methylation levels (5-methyl 2' deoxycytidine) in the liver and gonads of the three-spine stickleback (Gasterosteus aculeatus). HBCD at 30 and 300 ng/L of water did not produce statistically significant differences in global genomic methylation in liver of female stickleback. On the other hand, the methylation inhibitor, 5-aza-2'-deoxycytidine, significantly lowered hepatic global methylation levels in these fish by 14% (P<0.05). The naturally occurring oestrogen, 17-beta oestradiol (E(2)) at 100 ng/L also decreased global DNA methylation levels in female liver but this effect was not statistically significant. In contrast, both E(2) and 5AdC caused statistically significant (P<0.001 and P<0.01 respectively) global genomic hypermethylation in the gonads of male sticklebacks although the increase seen in the female gonads was not statistically significant. The male gonad effect though unexplained may potentially be an indirect response to hypomethylation in other tissues (such as the liver) and may have important implications regarding oestrogenic effects in fish. The contrasting effects of HBCD and E(2) on global DNA methylation in stickleback should contribute to the integrated risk assessment of these environmental chemicals.     

Physical basis of coastal adaptation on tropical small islands

Small tropical islands are widely recognized as having high exposure and vulnerability to climate change and other natural hazards. Ocean warming and acidification, changing storm patterns and intensity, and accelerated sea-level rise pose challenges that compound the intrinsic vulnerability of small, remote, island communities. Sustainable development requires robust guidance on the risks associated with natural hazards and climate change, including the potential for island coasts and reefs to keep pace with rising sea levels. Here we review these issues with special attention to their implications for climate-change vulnerability, adaptation, and disaster risk reduction in various island settings. We present new projections for 2010–2100 local sea-level rise (SLR) at 18 island sites, incorporating crustal motion and gravitational fingerprinting, for a range of Intergovernmental Panel on Climate Change global projections and a semi-empirical model. Projected 90-year SLR for the upper limit A1FI scenario with enhanced glacier drawdown ranges from 0.56 to 1.01 m for islands with a measured range of vertical motion from ?0.29 to +0.10 m. We classify tropical small islands into four broad groups comprising continental fragments, volcanic islands, near-atolls and atolls, and high carbonate islands including raised atolls. Because exposure to coastal forcing and hazards varies with island form, this provides a framework for consideration of vulnerability and adaptation strategies. Nevertheless, appropriate measures to adjust for climate change and to mitigate disaster risk depend on a place-based understanding of island landscapes and of processes operating in the coastal biophysical system of individual islands.