Biochemical examination of fetal skin biopsy specimens obtained by fetoscopy: Use of the method for analysis of keratins and filaggrin

This paper describes a method for biochemical analysis of proteins from fetal skin biopsy samples. The method has wide potential application for diagnosis of disorders with a known protein abnormality detectable by protein staining or a specific antibody. Analysis requires a single 1 mm biopsy, is rapid (2 days) and extremely sensitive. In the present study, fetal skin biopsies from normal fetuses and a fetus at risk for lamellar ichthyosis were obtained. The epidermis or hairs with attached follicular cells were dissected from the remaining skin. Proteins were extracted and separated by SDS-polyacrylamide gel electrophoresis. Proteins from duplicate gels were transferred to nitrocellulose and immunostained for the acidic and basic keratins and for the keratin filament associated protein, filaggrin, using monoclonal antibodies. All samples contained keratins typical of fetal epidermis at 20 weeks gestation. Presence of filaggrin is variable at this age and depends on the presence of keratinized cells of hair canals. No keratin abnormalities in the fetus at risk for lamellar ichthyosis were detected, however, in one presumably normal biopsy, an abnormally low proportion of the 67 kd keratin and the presence of follicular keratins were evident. These results demonstrate that biochemical analysis of fetal biopsies is possible, thus increasing the diagnostic potential of the fetal biopsy procedure for disorders in which a known protein or antigen is altered in utero.     

Deforestation Predicts the Number of Threatened Birds in Insular Southeast Asia

The world's tropical forests are being cleared rapidly, and ecologists claim this is causing a massive loss of species. This claim has its critics. Can we predict extinctions from the extent of deforestation? We mapped the percentage of deforestation on the islands of the Philippines and Indonesia and counted the number of bird species found only on these islands. We then used the species-area relationship to calculate the number of species predicted to become globally extinct following deforestation on these islands. Next, we counted the numbers of insular southeast Asian endemic bird species considered threatened—i.e., those having "a high probability of extinction in the wild in the medium-term future"—in the latest summary Red Data Book. The numbers of extinctions predicted from deforestation and the numbers of species actually threatened are strikingly similar. This suggests we can estimate the size of the extinction crisis in once-forested regions from the extent of deforestation. The numbers of extinctions will be large. Without rapid and effective conservation, many of the species endemic to insular southeast Asia will soon be lost.     

Environ indicator sensitivity to flux uncertainty in a phosphorus model of Lake Sidney Lanier, USA

Effective environmental impact assessment and management requires improved understanding of the organization and transformation of ecosystems in which independent agents are linked through an intricate network of energy, matter, and informational interactions. While advances have been made, we still lack a complete understanding of the processes that create, constrain, and sustain ecosystems. Network environ analysis (NEA) provides one approach for building novel ecosystem insights, but it is model dependent. As ecological modeling is an imprecise art, often complicated by inadequate empirical data, the utility of NEA may be limited by model uncertainty. Here, we investigate the sensitivity of NEA indicators of ecosystem growth and development to flow and storage uncertainty in a phosphorus model of Lake Sidney Lanier, USA. The indicators are total system throughflow (TST), total system storage (TSS), total boundary input (Boundary), Finn cycling index (FCI), ratio of indirect-to-direct flows (Indirect/Direct), indirect flow index (IFI), network aggradation (AGG), network homogenization (HMG), and network amplification (AMP). Our results make two primary contributions. First, they demonstrate that five of the indicators – FCI, Indirect/Direct, IFI, AGG and HMG – are relatively robust to the flow and storage uncertainty in the Lake Lanier model. This stability lets us draw robust conclusions about the Lake Lanier ecosystem organization (e.g., phosphorus flux in the lake is dominated by internal processes) in spite of uncertainties in the model. Second, we show that the majority of the indicators co-vary and that most of their common variation could be mapped onto two latent factors, which we interpret as (1) system integration and (2) boundary influences.     

TOTAL SOLUTES LOADS AND GEOCHEMICAL YIELDS IN THE SASKATCHEWAN RIVER BASIN1

ABSTRACT: River solute loads have seldom been measured in very large, complex drainage basins, nor have the methods of calculating loads been critically examined. For sites in the Saskatchewan River Basin, Canada, rating curves were poor predictors of solute loads because correlations between discharge and total solutes concentration were weak (R² < 0.05 in most cases) and suffered from hysteresis. In contrast, the interval method produced reliable estimates in all seasons and sites tested, and was little affected by sampling schedule. The limit of precision (SE) for estimates of mean annual or seasonal solute load was 10–15 percent of the mean (5 percent in very small basins), reached with 10 years or more of data. Two-thirds or more of total annual solute load was transported during the open-water season, but the proportion carried during winter increased from 8 percent to 34 percent from the upstream to the downstream end of the basin, due to reservoirs retaining and mixing water. Annual loads of total solutes varied from 6.2 × 10⁴ tonnes in foothills tributaries to almost 4.0 × 10⁶ tonnes in the Saskatchewan River near the mouth. But, on an areal basis, the mountain and foothills region was the dominant solute source, producing 43–97 tonnes/km²/yr, compared with only 3–22 tonnes/km²/yr for prairie rivers. This difference is a consequence of greater rainfall and, hence, more rapid erosion in the mountains.     

In-situ bioremediation of hanford groundwater

The U.S. Department of Energy has generated liquid wastes containing radioactive and hazardous chemicals throughout the more than forty years of operation at its Hanford site in Washington State. Many of the waste components, including nitrate and carbon tetrachloride (CCl₄), have been detected in the Hanford groundwater. In-situ bioremediation of CCl₄ and nitrate is being considered to clean the aquifer. Preliminary estimates indicate that this technology should cost significantly less than ex-situ bioremediation and about the same as air stripping/granular activated carbon. In-situ bioremediation has the advantage of providing ultimate destruction of the contaminant and requires significantly less remediation time. Currently, a test site is under development. A computer-aided design tool is being used to design optimal remediation conditions by linking subsurface transport predictions, site characterization data, and microbial growth and contaminant destruction kinetics.     

STATUS AND PROSPECTS OF KAREZ IRRIGATION1

ABSTRACT: The karez is a traditional irrigation water source, consisting of hand-dug horizontal wells, that is still used in parts of South Asia, the Middle East, and North Africa. This paper describes the construction, management, and current problems of karez irrigation systems based on investigations conducted in Baluchistan Province, Pakistan. While karezes have served irrigation needs well in the past, they are now threatened by high costs of labor for construction and maintenance, and by the encroachment of tubewells which lower the water tables on which the karez systems depend. Possible methods for improving karez performance and needs for research are discussed.     

IDENTIFICATION AND ANALYSIS OF OUTDOOR RECREATION HABITATS: THE RECREATION HABITAT ANALYSIS METHOD1

ABSTRACT: This paper describes a concept for identification and ranking of outdoor recreation habitats using principles of ecology as a guide. The Habitat Evaluation Procedures (HEP) is a commonly used technique for assessment of human impacts on the vigor of wildlife species, and serves as the model for the Recreation Habitat Analysis Method (RHAM). Recreation activities are dependent on habitat attributes for their success just as are wildlife species. A sample model is described for recreation activities at large, multipurpose lakes. RHAM could be applied to recreation experiences in a variety of settings including smaller water bodies and riparian and terrestrial ecosystems.