Agrobacterium-mediated transformation of Nicotiana attenuata, a model ecological expression system

Summary. Research into the genetic basis of the ecological sophistication of plants is hampered by the availability of transformable systems with a wealth of well-described ecological interactions. We present an Agrobacterium-mediated transformation system for the model ecological expression system, Nicotiana attenuata, a native tobacco that occupies the post-fire niche in the Great Basin Desert of North America. We describe a transformation vector and a transformation procedure that differs from the standard cultivated tobacco transformation protocols in its use of selectable markers, explants, media and cultivation conditions. We illustrate its utility in the transformations with genes coding for key enzymes in the oxylipin cascade (lipoxygenase, allene oxide synthase, hydroperoxide lyase) in antisense orientations and present high-throughput screens useful for the detection of altered phenotypes for the oxylipin cascade (green leaf volatiles and jasmonic acid after wounding). Received 14 March 2002; accepted 10 May 2002     

Conceptualizing controversies in the EU circular bioeconomy transition

The transition towards a circular bioeconomy (CBE) in the European Union is not without contestation. In particular, research has highlighted potential trade-offs of the large-scale production of bio-resources, for instance with environmental quality goals. To date, however, it remains underexplored in the CBE literature how controversies develop throughout a transition process. To address this gap, this paper explores where controversies are situated in a transition, how they change throughout, and how they influence the transition process. First, we suggest that controversies can be situated on and between different system layers within a transition. Second, we offer an explanation of how controversies evolve, as actors confirm, integrate, disintegrate and polarize underlying storylines. Third, these controversies can have both productive and unproductive outcomes while they unfold throughout a transition. We illustrate this understanding with the example of biorefineries as CBE key technology and discuss a research agenda on controversies in sustainability transitions.     

Enhanced removal of mercury and lead by a novel and efficient surface-functionalized imogolite with nanoscale zero-valent iron material

Environmental Science and Pollution Research - A novel hybrid nanomaterial, nanoscale zero-valent iron (nZVI)-grafted imogolite nanotubes (Imo), was synthesized via a fast and straightforward...