Phylogenetic relationships of the intercellular fish pathogenIchthyophonus hoferi and fungi,choanoflagellates and the rosette agent

Ichthyophonus hoferi Plehn and Mulsow, 1911 is thought to be one of the few pathogenic fungal infections of marine fish. The result of an attack is severe epizootics in herring stocks with drastic reduction in the population as a consequence. The exact phylogenetic position of the genusIchthyophonus is not known. In the present study, a combination of molecular data, ultrastructure and biochemical characters were utilized to investigate the phylogeny ofI.hoferi. The genomic DNA encoding the small subunit ribosomal RNA (18S rRNA) was amplified and sequenced. Comparisons with other eukaryotic 18S rRNA sequences indicate thatI. hoferi is not a member of the Fungi. In both the parsimony and the neighborjoining trees,I. hoferi is the sister taxon to the rosette agent. The clade encompassingI.hoferi and the rosette organism is the sister group to the chanoflagellate clade in the neighbor-joining tree, while in the parsimony tree theI. hoferi/rosette clade is equally distant to both the choanoflagellate and animal clades. Transmission electron microscopy showed thatI. hoferi has a defined cell wall, an endoplasm that consists of a fine granulated matrix with numerous ribosomes, several nuclei, vacuoles of varying density distributed throughout the cell, and mitochondria with tubular cristae. The cell wall ofI. hoferi contains chitin.     

Macroalgal growth in nutrient-enriched estuaries: A biogeochemical and evolutionary perspective

Increased nutrient loading of a body of water leads to an increase in macroalgal growth. It is generally observed, however, that certain species thrive more than others, capitalising on the increased nutrient availability. These are usually small, fast-growing, ephemeral algae that often appear as nuisance blooms. This article discusses the correlation between nutrient increase and macroalgal bloom formation. From a historical perspective, the evolutionary history of nuisance macroalgae, and the habits available to them prior to anthropogenic influences on estuarine geochemistry are considered. The occurrence of macroalgal genera whose distribution is commonly related to estuaries with high nutrient loading is discussed, along with evidence suggesting that the ecophysiological traits of r-selected, ephemeral algae largely contribute to their ecological success and bloom-formation at nutrient-enriched sites.     

Seasonality of cooling tower drift in vegetation at ORGDP

Cooling tower operation at the Oak Ridge Gaseous Diffusion Plant (ORGDP) requires the addition of a chromated corrosion inhibitor to the recirculating cooling water (RCW) system. Fluctuations in the RCW flow rates of the ORGDP cooling towers correlates with operational levels of the enrichment plant. Drift fraction is a function of the RCW flow rate, with variations in chromium deposition reflected in vegetation contamination. Fescue grass and litter were collected in the plant environs along a distance gradient, during different seasons, and analyzed for chromium. Foliar chromium concentrations were maximum (1390 μg/g) during the winter months, decreasing in spring and summer months (190 and 173 μg/g, respectively) at 13 m as the demand for cooling decreased with lower plant operations. In contrast, concentrations in litter increased with time, ranging from 894 μg/g at 13 m during the winter to 1890 and 2140 μg/g during the summer and fall months. The accumulation of chromium in the litter component was likely related to more exchange sites associated with increased litter biomass due to the seasonal senescence of foliage, whereas decreased accumulations on foliage reflected high mobility of the drift residue due to leaching and a short lifespan of individual leaves. In addition to operational considerations, the data illustrate the importance of seasonal considerations in the use of biological materials in a monitoring program.     

Precipitation-driven decrease in wildfires in British Columbia

Trends of summer precipitation and summer temperature and their influence on trends in summer drought and area burned in British Columbia (BC) were investigated for the period 1920–2000. The complexity imposed by topography was taken into account by incorporating high spatial resolution climate and fire data. Considerable regional variation in trends and in climate–fire relationships was observed. A weak but significant increase in summer temperature was detected in northeastern and coastal BC, whereas summer precipitation increased significantly in all regions—by up to 45.9 %. A significant decrease in province-wide area burned and at the level of sub-units was strongly related to increasing precipitation, more so than to changing temperature or drought severity. A stronger dependence of area burned on precipitation, a variable difficult to predict, implies that projected changes in future area burned in this region may yield higher uncertainties than in regions where temperature is predominantly the limiting factor for fire activity. We argue that analyses of fire–climate relationships must be undertaken at a sufficiently high resolution such that spatial variability in limiting factors on area burned like precipitation, temperature, and drought is captured within units.     

Maximising household waste recycling at civic amenity sites in Lancashire, England

In the UK, all local authorities have been set statutory performance standards for the recycling and composting of household waste. As part of their strategy for achieving these standards, Lancashire County Council (LCC) has made a number of alterations to the layout and facilities available at civic amenity sites (renamed Household Waste Recycling Centres (HWRCs)) in the county and tried to create a more positive image of the sites for members of the public. This study consisted of two surveys carried out in order to establish how to maximise household waste recycling at the 26 HWRCs in Lancashire. A telephone survey was carried out amongst all Lancashire's HWRC attendants in order to establish the effects of recent site improvements and to provide an indication of overall customer satisfaction. An on-site questionnaire survey was conducted amongst HWRC customers on five selected sites, over three separate seasons, incorporating comparative weekend and weekday surveys at each site. This survey investigated public opinion of HWRCs in Lancashire, as well as exploring effective methods that would assist customers in maximising the amount of recycling at HWRCs. The attendants' survey revealed that site attendants were actively encouraging customers to segregate waste more efficiently at the HWRCs, and that a recycling-related bonus scheme had provided an incentive towards this effort. Site attendants have a key role in educating the public about waste segregation and the effective use of HWRCs. Lancashire HWRC users were mostly male, used private cars to transport waste and showed high levels of customer satisfaction. Practical changes to encourage more recycling at HWRCs were clearly identified in the customers' survey; these include longer opening hours and the provision of boxes to enable pre-separation of waste. The study has clearly shown how the results of user surveys can provide practical assistance in the development of improved sustainable waste management strategies.     

J-value analysis of different regulatory limits for workers and the public

The J-value technique allows an objective determination to be made of the resources that should be applied cost effectively to improve heath and safety. This is essential if capabilities are to be employed optimally and risks reduced in a way that reflects their severity. Although other considerations such as good practice and socio-political influences may affect a final decision on the resources to be sanctioned, the incorporation of these additional factors should be made transparent if the decision is no longer to be based on cost effectiveness. The J-value provides an objective criterion by which to judge when “reasonable practicability” has been achieved in committing resources for safety improvement, which is the legal requirement under health and safety law in the UK.Moreover, the J-value methodology also allows other related issues to be addressed objectively. Regulatory bodies apply different limits for workers and the general public, with higher risks being permitted for workers. Although a factor of about 10 has been used in several contexts, no objective rationale has been developed for this particular figure until now. However, it is shown that application of the J-value analysis can provide a justification for a ratio of workers’ risk to public risk of approximately this size if certain reasonable assumptions are made. Thus the paper provides the first quantitative explanation for the different levels of protection demanded by regulators nationally and internationally for workers and public.     

Leader self‐awareness: An examination and implications of women's under‐prediction

Self‐awareness represents an important aspect of leadership. However, past research on leader self‐awareness has focused on one component of self‐awareness, self versus others' ratings, leaving the second component, the ability to anticipate the views of others, largely neglected. We examined this second component of self‐awareness by focusing on women leaders who have been found to under‐predict how others rate them. In two studies, we measured how women leaders anticipate the views of their bosses in regard to their leadership. In Study 1, 194 leaders rated their leadership, were rated by their bosses, and then predicted how their bosses rated their leadership. While we found that women under‐predict their boss ratings compared with men, we did not find that boss gender or feedback played a role in this under‐prediction. In Study 2, 76 female leaders identified (via open‐ended questions) possible reasons and consequences of under‐prediction for women in organizations. Results from Study 2 reveal the following: (1) the reasons for women's under‐prediction include a lack of self‐confidence, differences in feedback needs, learned gender roles, and self‐sexism; and (2) the perceived consequences of under‐prediction are negative for both women and the organization. Copyright © 2013 John Wiley & Sons, Ltd.