Use of Remote Sensing Techniques to Determine the Effects of Grazing on Vegetation Cover and Dune Elevation at Assateague Island National Seashore: Impact of Horses

The effects of grazing by feral horses on vegetation and dune topography at Assateague Island National Seashore were investigated using color-infrared imagery, lidar surveys, and field measurements. Five pairs of fenced and unfenced plots (300 m²) established in 1993 on sand flats and small dunes with similar elevation, topography, and vegetation cover were used for this study. Color-infrared imagery from 1998 and field measurements from 2001 indicated that there was a significant difference in vegetation cover between the fenced and unfenced plot-pairs over the study period. Fenced plots contained a higher percentage of vegetation cover that was dominated by American beachgrass (Ammophila breviligulata). Lidar surveys from 1997, 1999, and 2000 showed that there were significant differences in elevation and topography between fenced and unfenced plot-pairs. Fenced plots were, on average, 0.63 m higher than unfenced plots, whereas unfenced plots had generally decreased in elevation after establishment in 1993. Results demonstrate that feral horse grazing has had a significant impact on dune formation and has contributed to the erosion of dunes at Assateague Island. The findings suggest that unless the size of the feral horse population is reduced, grazing will continue to foster unnaturally high rates of dune erosion into the future. In order to maintain the natural processes that historically occurred on barrier islands, much larger fenced exclosures would be required to prevent horse grazing.     

A Quantitative Safety Assessment Model For Transgenic Protein Products Produced In Agricultural Crops

Transgenic plants are now being used to develop pharmaceutical and industrial products in addition to their use in crop improvement. Using confinement requirements, these transgenic plants are grown and processed under conditions that prevent intermixing with commodity crops. Regulatory agencies in the United States have provided guidance of zero tolerance of these new industrial crops with commodity crops. While this is a worthy goal, it is theoretically unattainable. In spite of the best containment practices, there is a potential risk using any system of production due to unforeseen incidences including natural disasters or exposure to workers. The precautionary principle has been used for numerous regulated articles in addressing the potential risks of new products and technology based on a risk assessment in similar situations. We present here a risk assessment model that could be used as a start to develop an accepted model for the industry. The model is based on current risk models used for other regulated articles, but adapted for these types of products. This could be used to determine action levels in the event of an unintended exposure or to ensure that detection or confinement methods are adequate to avoid risks. As an example, aprotinin, a therapeutic protein now being produced in maize, was evaluated for potential risk to humans using this model.     

Spectral analysis of forest model time series

The use of spectral analysis to elucidate the cyclic behavior in time series generated by a forest stand growth simulation model is discussed. A stand-level simulator, FORET, for an Appalachian deciduous forest is described. An estimate of the power spectral density of the total biomass time series is calculated. The power spectral density estimate indicates a dominant cyclic behavior with a period of about 200 years. In addition the spectral density is approximately bandlimited. This characteristic makes possible the application of the sampling theorem for analysis of sampling rates.     

Automatic sorting of ichthyoplankton; Factors controlling plankton density in gradients of silica

Following isopycnic sedimentation in density gradients of colloidal silica (Ludox AM), zooplankton segregated into 3 broad bands of increasing density which included, respectively, fish eggs, invertebrates, and fish larvae. The zone of fish eggs was contaminated with Sagitta spp. and the zone of fish larvae was overlapped partially by mysids and euphausids. We have sought to minimize contamination through modifications of the silica gradients which might shift banding densities of the ichthyoplankton or of the contaminating invertebrates. Density shifts in fact occurred in different commercial formulations of silica sols and in gradients modified by small amounts of polymers, osmotica or charged small molecules. Sagitta were specifically shifted out of the zone of fish eggs by the addition, of 1% w/v dextran sulfate or in a silica sol composed of very small particles of silica (Nalcoag 1115). Fish larvae were shifted to higher densities by the addition of 1% w/v trimetaphosphate or 1% w/v dextran sulfate. Additions of 1% w/v polyvinyl alcohol, and of 10 mM glycine, and potassium phosphate had intermediate effects. The same banding densities of plankton in silica gradients were reached in less than 10 h at one gravity, independent of the immediate pretreatment of the sample.     

Metabolism of crufomate (4-tert-butyl-2-chlorophenyl methyl methylphosphoramidate) administered topically to a sheep.

A sheep dosed topically with 14C-crufomate (4-tert-butyl-2-chlorophenyl methyl methylphosphoramidate) excreted 45.5% of the 14C dose in the urine within 9 days. The feces contained 1.2% and the carcass 40.4% (this included the 37.7% of the dose remaining on the skin in the dosing area) of the dose. At sacrifice, the fat, liver, kidney, lung, and skin (where the dose was applied) contained the highest concentrations of 14C. Fourteen urinary metabolites were isolated and characterized by mass spectrometry. The metabolic reactions involved were oxidations of the t-butyl moiety, O-demethylation, replacement of the H-N-CH3 moiety with a hydroxyl group, oxidation of the N-methyl group to yield N-formyl phosphoramidates, hydrolysis of the phosphoramidate moiety to yield phenols, conjugation with glucuronic acid and combinations of these reactions.