Variable within-brood maternal provisioning in newly extruded embryos of Homarus gammarus

Offspring quality of decapod crustaceans has been widely studied, with special emphasis on the sources of variability determining embryonic and larval quality. Nevertheless, maternal provisioning has commonly been overlooked as a potential source of offspring within-brood variability. In the present study, the existence of variable maternal provisioning was assessed through the analysis of the fatty acid (FA) profile of newly extruded embryos from different regions of the brooding chamber of Homarus gammarus. Significant differences in the FA profile of embryos sampled from different pleopods and sides of the brooding chamber were recorded. Significant deviations of the overall mean of each surveyed female were also observed for essential FA, particularly 20:4n-6, 20:5n-3 and 22:6n-3. Lipid energy available to fuel embryogenesis also varied among embryos sampled from different regions of the brooding chamber. Results suggest variable female investment at oocyte production, which may be amplified during the incubation period of developing embryos by differential lipid catabolism. For the first time, maternal provisioning is evidenced as an additional source for within-brood variability in the FA profile of embryos.     

New aspect in seagrass acclimation: leaf mechanical properties vary spatially and seasonally in the temperate species Cymodocea nodosa Ucria (Ascherson)

Seagrasses may acclimate to environmental heterogeneity through phenotypic plasticity. In contrast to leaf morphology, which has been a central point in seagrass acclimation studies, plasticity in leaf biomechanics and fibre content is poorly understood, despite being crucial in plant ecological performance, especially regarding physical forces. We hypothesised that mechanical traits (e.g. breaking force, strength, toughness, and stiffness) and fibre content of seagrass leaves vary as morphology does under differential environments. Cymodocea nodosa was seasonally monitored at three locations around Cádiz Bay (southern Spain) with hydrodynamic regime as the most noticeable difference between them. Leaves showed plasticity in both morphology and mechanical traits, with wave-exposed individuals presenting short but extensible and tough leaves. Leaf fibre content was invariant along the year and with little spatial variability. Cross-sectional area rather than material properties or fibre content differentiates leaf mechanical resistance. Seagrass capacity to thrive under a range of mechanical forces may be dictated by their plasticity in morpho-biomechanical traits, a key element for the hydrodynamical performance and, hence, for species colonisation and distribution.     

How age and sex drive the foraging behaviour in the king penguin

As predicted by life history theory, once recruited into the breeding population and with increasing age, long-lived animals should be able to manage more efficiently the conflict between self-maintenance and reproduction. Consequently, breeding performances should improve with age before stabilizing at a certain level. Using temperature–depth recorders and isotopic analysis, we tested how age affects the foraging behaviour of king penguin Aptenodytes patagonicus during one trip in the chick-rearing phase. Depending on sex and age, king penguins expressed two different foraging strategies. Older birds gained more daily mass per unit body mass than younger ones. Older females conducted shorter trips, dived deeper and performed more prey pursuits. They also had higher blood levels of δ¹⁵N than younger individuals and males indicating sex- and age-specific dietary regimes. However, we found no differences in carbon isotopic signature, suggesting that individuals exploited the same foraging areas independently of sex and age. Our results suggest that king penguins are able to increase the quantity of energy extracted with increasing age and that such a strategy is sex-related. Our study is the first to reveal of an interaction between age and sex in determining foraging efficiency in king penguins.     

Photosynthetic and morphological photoacclimation of the seagrass Cymodocea nodosa to season, depth and leaf position

The photoacclimation capacity of the seagrass Cymodocea nodosa was evaluated considering temporal (i.e. seasonal) and spatial (i.e. depth and within-leaf position) factors of variation. Changes along the leaf were measured in a population growing along a depth gradient (from intertidal to subtidal) in Cadiz Bay (Southern Spain) from 2004 to 2005. Photoacclimation was evaluated by photosynthesis (P–E curves), pigment content and leaf morphology. Plants of Cymodocea nodosa showed large physiological and morphological plasticity (mean %CV = 35.8 ± 3.4) according to the three factors considered. Seasonal patterns appeared for photosynthesis, respiration, pigment content and morphology. Nevertheless, seasonal patterns were not consistent with depth or leaf portions. The resulting data set offered different information depending on the analysis conducted; when only one factor (season, depth or leaf portion) was considered, some tendencies observed in the 3-way full design were masked. Accordingly, considering spatio–temporal variability is crucial when describing photoacclimation and estimating productivity in seagrass meadows.     

Genetic structure of wild European populations of the invasive Pacific oyster Crassostrea gigas due to aquaculture practices

As a result of aquaculture activities, Pacific oysters Crassostrea gigas (Thunberg, 1793) have invaded European coasts. Using seven microsatellites, we found virtually no genetic differentiation between natural populations throughout the European range (from the south of the Wadden Sea (the Netherlands) to the south of France) and French cultivated oysters. The genetic homogeneity of Pacific oyster samples appears to be the result of repeated transfers from same seed stocks made for aquaculture and, to a lesser extent, widespread dispersal due to specific biological traits of this species. The only genetic differentiation of Sylt population in the north of the Wadden Sea (Germany) suggests a stronger, persistent impact of ongoing supply of new genetic material from hatchery production, corresponding to seeds selection made by breeders. All of our genetic data highlighted the importance of aquaculture practices on the genetic structure of the keystone invader C. gigas in Europe.     

Ecological niche segregation among five toothed whale species off the NW Iberian Peninsula using ecological tracers as multi-approach

This study aims to assess niche segregation among the five main toothed whales that frequent the NW Iberian Peninsula waters: the common dolphin, the harbour porpoise, the bottlenose dolphin, the striped dolphin and the long-finned pilot whale. We used cadmium (Cd) and stable isotope ratios (δ¹³C and δ¹⁵N) as ecological tracers to assess degree of segregation in diet/trophic level and in foraging habitat, over various time-scales. δ¹³C values highlighted different habitats, while Cd concentrations highlighted feeding differences between oceanic and neritic species. Moreover, δ¹⁵N values suggest different trophic levels of prey targeted within oceanic and neritic species. Hence, results revealed long-term ecological segregation among five toothed whales that coexist in the NWIP and demonstrated the ability of ecological tracers to discriminate ecological niches among closely related species.     

Experimental evidence for internal predation in microzooplankton communities

The fate of microzooplankton production, whether it is channeled to mesozooplankton or recycled within the microbial food web, has major implications for the oceanic carbon cycle. The aim of this study was to estimate internal predation within naturally occurring microzooplankton communities. A dilution series based on the Landry and Hasset technique was created by mixing 200-μm-screened water (used as whole water) with 5-μm-screened seawater due to the dominance of pico- and small nanoplankton at our study site. This modification of the original technique allows for gradual reduction in microzooplankton abundance and thus internal predation while maintaining sufficient phytoplankton prey levels for microzooplankton growth in diluted treatments. Microzooplankton growth and mortality rates were calculated based on the changes in abundance during 24-h incubation. In the diluted treatments, microzooplankton growth rates were significantly higher (1.21 ± 0.20 day^?1 for ciliates and 0.88 ± 0.05 day^?1 for heterotrophic dinoflagellates) compared to those in whole seawater where microzooplankton abundance remained unchanged or even declined over time. Approximately 79 % of microzooplankton production was consumed within the microzooplankton, with aloricate ciliates being the most vulnerable to predation. These findings support the assumption that trophic interactions between microzooplankton can be an important factor controlling their production and, thus, energy transfer in picoplankton-dominated pelagic ecosystems.     

Temporal patterns of settlement, recruitment and post-settlement losses in a rocky reef fish assemblage in the South-Western Mediterranean Sea

Post-settlement events can significantly alter the density distribution of settlers and subsequently the adult population structure. The temporal and inter-annual variability of settlement and the effects of mortality on recruitment were investigated across 2 years in the vicinities of Cabo de Palos–Islas Hormigas Marine Reserve by visual census and light trap sampling. Settlement was seasonal with greater species richness and abundance in summer. Although temporal synchronization was observed between larval supply and settlement, densities of settlers could not be predicted from post-larval abundances. Timing of settlement was consistent between years but with high inter-annual variation in abundance. High mortality (~80 %) and general decoupling between post-larval and settlement stages suggest that early mortality is driving such patterns. Nevertheless, indications of habitat-mediated mortality were found for benthic species such as Symphodus and Diplodus, highlighting the importance of habitat in shaping population demography.     

Sublethal concentrations of azinphos-methyl induce biochemical and morphological alterations in Rhinella arenarum embryos

Considering that amphibians are good sentinels of environmental conditions, Rhinella arenarum embryos were used to investigate the effects of sublethal concentrations of the organophosphorus insecticide azinphos-methyl, focusing on its anticholinesterasic or pro-oxidant actions and its possible connection with the appearance of morphological alterations. Early amphibian embryos exposed to azinphos-methyl displayed a protective response through glutathione S-transferase induction, along with superoxide dismutase inhibition. At intermediate embryonic stages, embryos exposed to azinphos-methyl displayed superoxide dismutase inhibition and morphological alterations, although cholinesterase activity was not altered, suggesting that molecular targets other than cholinesterase were involved in the development of morphological alterations. At the end of embryonic development, decreases in reduced glutathione and cholinesterase inhibition were observed, along with a significant increase in the number of malformed embryos. The connection between biochemical alterations and the appearance of malformations was not evident in R. arenarum embryos. However, increased glutathione S-transferase and decreased superoxide dismutase activities could be considered as early markers of exposure to azinphos-methyl. The results obtained demonstrate that sublethal concentrations of azinphos-methyl are a serious threat to toad embryos in their natural habitats because biochemical and morphological alterations could impair their ability to deal with environmental stresses.