The effects of elevated carbon dioxide levels on a Vibrio sp. isolated from the deep-sea

Introduction

The effect of oceanic CO₂ sequestration was examined exposing a deep-sea bacterium identified as Vibrio alginolyticus (9NA) to elevated levels of carbon dioxide and monitoring its growth at 2,750 psi (1,846 m depth).

Findings

The wild-type strain of 9NA could not grow in acidified marine broth below a pH of 5. The pH of marine broth did not drop below this level until at least 20.8 mM of CO₂ was injected into the medium. 9NA did not grow at this CO₂ concentration or higher concentrations (31.2 and 41.6 mM) for at least 72 h. Carbon dioxide at 10.4 mM also inhibited growth, but the bacterium was able to recover and grow. Exposure to CO₂ caused the cell to undergo a morphological change and form a dimple-like structure. The membrane was also damaged but with no protein leakage.     

Biochemical and genotoxic effects in women exposed to pesticides in Southern Ecuador

Environmental Science and Pollution Research - Toxicity refers to the potential of a substance such as a pesticide to cause damage to the structure or functions of an exposed organism. Pesticides...     

Identification of sex and parasitism via pheromones by the Ozark zigzag salamander

Chemical communication appears to be the primary mode of information transfer among woodland salamanders of the genus Plethodon. These pheromones have been shown to convey a wide variety of information, and are used to establish territorial boundaries. Responses to pheromones can include changes in both behavior and energy consumption. The ability of Ozark zigzag salamanders, Plethodon angusticlavius, to detect the sex of a conspecific through chemical substrate markings has not been assessed. In addition, some populations of P. angusticlavius are naturally infected with the ectoparasitic mite Hannemania eltoni. Parasitism can impact both the behavior and metabolism of the host as well as the behavior of others towards the parasitized individual. We examined whether sex and parasitism of the pheromone donor and receiver influenced behavioral and metabolic responses in P. angusticlavius. In laboratory trials, behavior of P. angusticlavius was influenced by the sex and parasite load of the pheromone donors and receivers. Behavioral responses were also influenced by whether the receiver was in its own territory or in the territory of the pheromone donor. Metabolic responses were not affected by the sex or parasite load of the pheromone donor, but males had higher metabolic rates than females, even after adjusting for differences in body size. Overall, our study indicates that both sex and parasitism can be detected via pheromones in this species, and the nature of the response depends on residency status, sex, and parasite load of the receiver.     

Landscape Change and Ecosystem Classification in a Municipal District of a Small City (Isernia,Central Italy)

Landscape changes taking place from 1954 to 1992 in the muncipal district of Isernia city (Central Italy) were described in relation to a system of ecosystem classification. Isernia municipal district was selected for study because recent historic changes in this area represent a typical example of landscape transformation similar to many small cities of Italy and other Mediterranean countries. To assess overall changes, three land cover maps (scale 1:25,000) were derived from panchromatic aerial photographs and field surveys. These were then digitalised in a Geographic Information System. A Land Facet (LF) map was derived by combining a phytoclimatic, a lithostatigrafic and a topographic map, and then digitalised as data layers in the same GIS. Results demonstrated two main landscape transformation trends: forest and semi-natural areas increased (8%), whereas agricultural areas decreased (12%). The urban area was relatively small during the entire analysed period, growing from 1% in 1954, to just 5% in 1992. Forest coverage was significant on reliefs, on hillside ecosystems such as limestone and on clay and marl hills LF. Arable land was particularly significant in flat ecosystems with deeper soils, such as on recent alluvial plain LF. These temporal changes were interpreted as being related to the replacement of traditional farming methods (grazing pastures) with more intensive methods (crop fields), especially on alluvial plains.     

Collective Action in the Management of a Tropical Dry Forest Ecosystem: Effects of Mexico’s Property Rights Regime

Dilemmas of natural resources governance have been a central concern for scholars, policy makers, and users. Major debates occur over the implications of property rights for common resources management. After the Mexican Revolution (1910–1917), land was distributed mainly as ejidos conceived as a hereditary but unalienable collective form of property. In 1992, a new Agrarian Law was decreed that allows individual ownership by removing various restrictions over the transfer of land. Scholars have examined the reform mainly focusing on land-tenure changes and environmental fragmentation. This study examines how the new ownership regime is affecting collective decision-making in ejidos located in a tropical dry forest (TDF) ecosystem. Information on decision-making processes before and after the 1992 reform was gathered through 52 interviews conducted in four ejidos selected along a gradient including agricultural, cattle-raising, and TDF use. The new individualized land property system reduced collective action in ejidos but did not trigger it. Collective action responses to the 1992 reform were buffered by self-organization each ejido already had. Heterogeneous users who shared a short history and showed little understanding of TDF and low dependence on its resources seemed to explain why ejidos have not been able to share a sense of community that would shape the construction of institutions for the collective management of forest resources. However, when a resource is scarce and highly valuable such as water the same users showed capacities for undertaking costly co-operative activities.     

Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina

Levels of glyphosate were determined in water, soil and sediment samples from a transgenic soybean cultivation area located near to tributaries streams of the Pergamino-Arrecifes system in the north of the Province of Buenos Aires, Argentina. Field work took into account both the pesticide application and the rains occurring after applications. The pesticide was analysed by HPLC-UV detection, previous derivatization with 9-fluorenylmethylchloroformate (FMOC-Cl). In addition, SoilFug multimedia model was used to analyse the environmental distribution of the pesticides. In the field, levels of glyphosate in waters ranged from 0.10 to 0.70mg/L, while in sediments and soils values were between 0.5 and 5.0mg/Kg. Temporal variation of glyphosate levels depended directly on the time of application and the rain events. The results obtained from the application of the model are in accordance with the values found in the field.     

Nacre and false nacre (foliated aragonite) in extant monoplacophorans (=Tryblidiida: Mollusca)

Extant monoplacophorans (Tryblidiida, Mollusca) have traditionally been reported as having an internal nacreous layer, thus representing the ancestral molluscan condition. The examination of this layer in three species of Neopilinidae (Rokopella euglypta, Veleropilina zografi, and Micropilina arntzi) reveals that only V. zografi secretes an internal layer of true nacre, which occupies only part of the internal shell surface. The rest of the internal surface of V. zografi and the whole internal surfaces of the other two species examined are covered by a material consisting of lath-like, instead of brick-like, crystals, which are arranged into lamellae. In all cases examined, the crystallographic c-axis in this lamellar material is perpendicular to the surface of laths and the a-axis is parallel to their long dimension. The differences between taxa relate to the frequency of twins, which is much higher in Micropilina. In general, the material is well ordered, particularly towards the margin, where lamellae pile up at a small step size, which is most likely due to processes of crystal competition. Given its morphological resemblance to the foliated calcite of bivalves, we propose the name foliated aragonite for this previously undescribed biomaterial secreted by monoplacophorans. We conclude that the foliated aragonite probably lacks preformed interlamellar membranes and is therefore not a variant of nacre. A review of the existing literature reveals that previous reports of nacre in the group were instead of the aragonitic foliated layer and that our report of nacre in V. zografi is the first undisputed evidence of nacre in monoplacophorans. From the evolutionary viewpoint, the foliated aragonite could easily have been derived from nacre. Assuming that nacre represents the ancestral condition, as in other molluscan classes, it has been replaced by foliated aragonite along the tryblidiidan lineage, although the fossil record does not presently provide evidence as to when this replacement took place.     

Evaluating Pre‐ and Post‐Fire Peak Discharge Predictions across Western U.S. Watersheds

This study reviews five models commonly used in post‐fire hydrologic assessments: the Rowe Countryman and Storey (RCS), United States Geological Survey (USGS) Linear Regression Equations, USDA Windows Technical Release 55 (USDA TR‐55), Wildcat5, and U.S. Army Corps of Engineers (USACE) Hydrologic Modeling System (HEC‐HMS). The models are applied to eight diverse basins in the western United States (U.S.) (Arizona, California, Colorado, Montana, and Washington) affected by wildfires and assessed by input parameters, calibration methods, model constraints, and performance. No one model is versatile enough for application to all study sites. Results show inconsistency between model predictions for events across the sites and less confidence with larger return periods (25‐ and 50‐year events) and post‐fire predictions. The RCS method performs well, but application is limited to southern California. The USGS linear regression model has wider regional application, but performance is less reliable at the large recurrence intervals and post‐fire predictions are reliant on a subjective modifier. Of the three curve number‐based models, Wildcat5 performs best overall without calibration, whereas the calibrated TR‐55 and HEC‐HMS models show significant improvement in pre‐fire predictions. Results from our study provide information and guidance to ultimately improve model selection for post‐fire prediction and encourage uniform parameter acquisition and calibration across the western U.S.