A molecular fingerprint technique to detect pollution-related changes in river cyanobacterial diversity

Humans now have a strong influence on almost every major aquatic ecosystem, and our activities have dramatically altered the quality of receiving waters worldwide. Thus, there is a continuous need to develop and apply novel and effective technologies to detect, manage, and correct human-induced degradation of aquatic systems. In the present work, we evaluated the molecular approach using polymerase chain reaction (PCR)-temperature gradient gel electrophoresis (TGGE) to measure changes in cyanobacterial diversity along a pollution gradient in a river and compared it with that of using microscopic observations of field-fixed and cultured samples. The different 16S rDNA genes present in the cyanobacterial community of each sampling point of the river were separated by TGGE, giving a characteristic pattern of bands for each site. This pattern represents a "fingerprint" of the community, allowing direct comparisons of the different samples. The TGGE results revealed that the structure of the cyanobacterial community differed along the pollution gradient of the river. Microscopic and molecular approaches showed that cyanobacterial diversity decreased in a downstream direction. Similar results were obtained by the two methods, as indicated by the high correlation between them. We suggest PCR-TGGE could be a useful and rapidly applied technique for the routine analysis of changes in cyanobacterial diversity in response to pollution, which would allow us to monitor rivers in surveillance networks of watercourse quality.     

Uptake and accumulation of lead by plants from the Bo Ngam lead mine area in Thailand

A field survey of terrestrial plants growing on Bo Ngam lead mine area, Thailand, was conducted to identify species accumulating exceptionally high concentrations of lead. Plant and soil samples were collected from five areas. Lead concentrations in surface soil ranged from 325 to 142,400 mg/kg. The highest lead concentration in soil was found at the ore dressing plant area and lowest at a natural pond area. In different areas, the concentrations of lead in plants were different when comparing various study sites. A total of 48 plant species belonging to 14 families were collected from five sampling sites. Twenty-six plant species had lead concentrations more than 1000 mg/kg in their shoots. Three species (Microstegium ciliatum, Polygala umbonata, Spermacoce mauritiana) showed extremely high lead concentrations in their shoots (12,200-28,370 mg/kg) and roots (14,580-128,830 mg/kg).     

Ozone concentrations in the Central Wasatch Mountains of Utah

In the Salt Lake Valley, a June through August SUM60 value (sum of hourly average ozone concentrations > or =60 parts per billion by volume [ppbv]) of 25,000 ppbv-hr was exceeded in 9 yr between 1978 and 1998. Ozone concentrations in the nearby Central Wasatch Mountains were monitored to determine the potential for vegetation injury. The SUM60 value of 19,000 ppbv-hr in these mountains and peak hourly concentrations >100 ppbv suggests that ozone-sensitive species may be injured. Ozone concentrations in the mountains were greatest during periods of strong upslope winds from the Salt Lake Valley. Both SUM60 values and hourly average concentrations in the Central Wasatch Mountains were strongly correlated with those in the Salt Lake Valley, suggesting that data from valley stations could be used to estimate ozone in the mountains.     

HYDROLOGICAL UNDERSTANDING AND SOCIETAL ACTION1

ABSTRACT: Hydrology is both an applied practical science and a pure geophysical science. The goal of hydrology, as a geophysical science, is to achieve theories capable of explaining with satisfactory accuracy the phenomena of interest. Through the rapidly accelerating power and versatility of digital computing technology, theory development and application are immensely facilitated via increasingly sophisticated predictive modeling schemes, which are now the principal operating tools both for applied management hydrology and for basic geophysical hydrology. While this approach treats phenomena as classes or generalizations, social and behavioral scientists have long argued that human beings base their actions on percepts, i.e., on the concrete specifics of their experience. Thus, the commonly held ideal of basing policy, decisions, and public actions on the best possible science encounters a conflict in belief systems. A possible resolution of this dilemma lies in the use of observational components, which in concept-centered science serve as data to test or calibrate models. These components also serve as a great repository of natural experience that is closely attuned to the perceptual reality that propels societal action. Landscapes and sediments provide indices of real processes, whose occurrence can be expected by continuity to extend to present and future activity. More attention to research on such indices is warranted as a means of triggering perception-based action by responsible decision-makers. Grounded in reality, and tempered by their intrinsic fallibility, the scientifically powerful conceptual schemes (models) will then serve as guides to further action. The full societal benefit of hydrological science requires a balanced approach in which subdisciplines focused on environmental indices are afforded equal attention to those focused on conceptual idealization.     

Risk Analysis and Occurrence of Hepatitis E Virus (HEV) in Domestic Swine in Northeast Brazil

Anti-HEV antibodies were detected in animals from abattoir and in farms from northeast Brazil. Our results suggest that HEV is highly disseminated in the swine population and might present a great risk to animal handlers and for consumption of raw or undercooked meat and meat products in northeast Brazil.     

PRIVACY IN THE WORK PLACE: THE IMPACT OF BUILDING DESIGN

This study examines the impact of building design on privacy in two office environments at Gulfstream Aerospace Corporation, Inc., U.S.A. The findings offer a range of design resources that Facility Management at Gulfstream can utilize to manage privacy for their office workers. In a broader context, the theoretical considerations presented in this study, though still in their formative stage, offer additional insight into what office workers may think about privacy in the work environment. These theoretical considerations have far reaching implications for design professionals, and pending further research may prove cost effective.