Karyotypic characterization of the great sturgeon, Huso huso, by multiple staining techniques and fluorescent in situ hybridization

A karyotype analysis by several staining techniques was carried out on the great sturgeon, Huso huso (Linnaeus, 1758). The karyotype (2n = 118 ± 2) was composed of 42 pairs of meta-/submetacentric chromosomes and 17 pairs of acrocentrics/microchromosomes. Constitutive heterochromatin was mainly located at the centromeric regions of the acrocentric chromosomes. The biarmed chromosomes showed weak C-bands. Fluorescent staining with GC-specific chromomycin A₃ showed clearly recognizable fluorescent regions, whereas a more uniform base composition was revealed by the AT-specific 4,6-diamidino-2 phenylindole. After Ag-staining, nucleolar organizer regions could be observed on the short arms of two medium-sized submetacentrics and on two acrocentrics. Digoxigenated 28S and 5S rDNA probes, prepared from Acipenser naccarii DNA and hybridized to metaphase chromosomes, showed signals on six and two chromosomes, respectively. The telomeric sequence (TTAGGG) _n detected by FISH was located at both ends of each chromosome. Results are discussed in relation to karyotype organization and evolution in sturgeons. Received: 1 April 1998 / Accepted: 21 July 1998     

IFTCIP: An Integrated Optimization Model for Environmental Management under Uncertainty

In this study, an interval-fuzzy two-stage chance-constrained integer programming (IFTCIP) method is developed for supporting environmental management under uncertainty. The IFTCIP improves upon the existing interval, fuzzy, and two-stage programming approaches by allowing uncertainties expressed as probability distributions, fuzzy sets, and discrete intervals to be directly incorporated within a general mixed integer linear programming framework. It has advantages in uncertainty reflection, policy investigation, risk assessment, and capacity-expansion analysis in comparison to the other optimization methods. Moreover, it can help examine the risk of violating system constraints and the associated consequences. The developed method is applied to the planning for facility expansion and waste-flow allocation within a municipal solid waste management system. Violations of capacity constraints are allowed under a range of significance levels, which reflects tradeoffs between the system cost and the constraint-violation risk. The results indicate that reasonable solutions for both binary and continuous variables have been generated under different risk levels. They are useful for generating desired decision alternatives with minimized system cost and constraint-violation risk under various environmental, economic, and system-reliability conditions. Generally, willingness to take a higher risk of constraint violation will guarantee a lower system cost; a strong desire to acquire a lower risk will run into a higher system cost.     

Optimal Selection of Priority Development Areas Considering Tradeoffs Between Hydrology and Development Configuration

Mixed-integer linear programs are proposed for siting development and conservation areas in watersheds, addressing economic objectives (development perimeter and proximity) and ecological objectives. Links between watershed hydrology and ecology need not be well defined. Parameters for the linear programs are obtained from linearization of the SWAT hydrologic model.     

The 210Po and 210Pb levels in surface sediment samples in the Izmir Bay (Aegean Sea-Turkey)

Bottom sediments reflect in general the relative contamination of a sea area. Therefore, a great deal of monitoring work has been dedicated to the analysis of bottom sediments. Izmir Bay is a very important pollution centre in Turkish Aegean coast region due to a densely populated community, industrial complex and maritime transportation, and there are many streams flowing into the bay that pass through a number of industrial and agricultural areas. It had received the majority of domestic and industrial wastewaters until the wastewater treatment plant was constructed. It is well known that sediments play an important role as reservoirs of a fraction of the pollution in aquatic systems. Therefore, sediment samples were collected monthly from three stations which are located in the inner part of the bay during the period January to December 2003. Temporal variations and seasonal changes on their ²¹⁰Po and ²¹⁰Pb contents were examined, and the activity concentrations of ²¹⁰Po and ²¹⁰Pb were found to vary from 43±6 to 132±12 and 27±5 to 91±9 Bq kg^???1 dry wt, respectively. The highest values of those natural radionuclides were measured at Kar??yaka Station because of the current systems of the bay. Seasonally, the ²¹⁰Pb levels were found to increase during the winter time for all the stations.     

Effects of cement flue dusts from a Nigerian cement plant on air, water and planktonic quality

Effects of cement flue dust from Ewekoro cement Kilns were monitored at some aquatic receptor locations. High levels of total suspended particulates (TSPs) and atmospheric deposition rates (ADRs) were recorded within the factory compared to ancillary locations outside the factory. The TSP and ADR levels which were location dependent were significantly higher (P?<?0.05) during the dry periods than in the wet season. Irrespective of seasonal variations, the key elements in the emissions were Ca^2?+? and Fe^2?+?. The concentrations of Zn^2?+?, Mn^2?+? and Pb^?+? which were trace elements were significantly higher (P?<?0.05) in the deposited than in the airborne particulates. The planktonic flora and fauna of the river systems draining the area were poor with 16 phytoplanktonic and nine zooplanktonic species. Numerically, the phytoplanktons were dominated by diatoms (Bacillariophyta) with Synedra sp. being the most abundant species. The zooplanktonic fauna dominated by rotifers had Lecane curvicornis as a regular occurrence in all the three catchment rivers. The physicochemical parameters assayed were significantly higher (P?<?0.05) in the factory effluent discharges than in water samples from each of the catchment rivers. Seasonal variations inclusive, HCO $_{3}^{-}$ , CO $_{3}^{2-}$ , Ca^2?+? and Mg^2?+? constituted the major ionic component of water samples analysed irrespective of location. Alaguntan River which receives effluents directly from the factory had significantly higher levels (P?<?0.05) of the assayed ions than the other two rivers draining the cement factory catchment areas.     

Essential (Cu) and nonessential (Cd and Pb) metals in ichthyofauna from the coasts of Sinaloa state (SE Gulf of California)

With the aim of giving an overview on concentration and distribution of Cd, Cu, and Pb in fish from the coasts of Sinaloa state (SE Gulf of California), specimens with different feeding habits were collected in five locations. Sampling occurred between June 2003 and March 2004. Metal analyses on fish tissues were made by graphite furnace (Cd, Pb) and flame (Cu) atomic absorption spectrophotometry. Metal concentrations in tissues of carnivorous fish were grouped together and compared with corresponding concentrations in non-carnivorous fish; Cu and Pb levels were significantly (p?<?0.05) higher in liver of non-carnivorous species. Though no samples exceeded the maximum level set in international legislation for fish, from the perspective of the public health and considering the legal limits of fishery products for human consumption, Cu concentrations were exceeded (in tissues different from muscle) in four carnivorous and five non-carnivorous species according to the Australian legislation. In the case of Cd, two carnivorous species (Pomadasys leuciscus and Caulolatilus princeps) and one non-carnivorous species (Mugil cephalus), showed concentrations over the maximum level of 2 μg g^???1 dry weight considered in the Mexican legislation. Considering average amounts of fish consumption in Mexico, daily mineral intake (DMI) values for Cu and percentage weekly intake (PWI) of Cd and Pb were estimated; none of the analyzed metals in edible portion of analyzed fish could be detrimental to humans.     

Estimation of pollutant partition in sandy soils with different water contents

The objectives of this work were: (1) to identify an isotherm model to relate the contaminant contents in the gas phase with those in the solid and non-aqueous liquid phases; (2) to develop a methodology for the estimation of the contaminant distribution in the different phases of the soil; and (3) to evaluate the influence of soil water content on the contaminant distribution in soil. For sandy soils with negligible contents of clay and natural organic matter, contaminated with benzene, toluene, ethylbenzene, xylene, trichloroethylene (TCE), and perchloroethylene (PCE), it was concluded that: (1) Freundlich’s model showed to be adequate to relate the contaminant contents in the gas phase with those in the solid and non-aqueous liquid phases; (2) the distribution of the contaminants in the different phases present in the soil could be estimated with differences lower than 10% for 83% of the cases; and (3) an increase of the soil water content led to a decrease of the amount of contaminant in the solid and non-aqueous liquid phases, increasing the amount in the other phases.     

Organic speciation of atmospheric particles in Alvão Natural Park (Portugal)

PM₁₀ continental rural background aerosols were collected during a summer field campaign (August–September 2006) at Lamas de Ôlo in the upper zone of the Alvão Natural Park, a mountain region of northern Portugal. In addition to the determination of the carbonaceous content by a thermal–optical method, the organic speciation of aerosols was performed by gas chromatography–mass spectrometry in an effort to evaluate photo-oxidation products of biogenic volatile organic compounds and other markers for source characterization. The detailed analysis revealed relatively high concentrations of polyols and short-chain dicarboxylic, tricarboxylic, hydroxycarboxylic, and oxocarboxylic acids, many of which are thought to be indicators of secondary aerosol formation, accounting for about 70% of global chromatographically resolved mass. Major photo-oxidation products of α- and β-pinene have been detected. The tracers for the photo-oxidation of isoprene comprise two diastereoisomeric 2-methyltetrols, C₅-alkene triols, and 2-methylglyceric acid, which have only recently been elucidated. In addition, the occurrence of levoglucosan and other biomass combustion tracers indicates that the site was affected by wildfires. This source contributed to more than 80% of the organic carbon mass during a period of strong forest fire influence.     

Heavy metal pollution across sites affecting the intestinal helminth communities of the Egyptian lizard, Chalcides ocellatus (Forskal, 1775)

This study aimed to investigate the possible effects of heavy metal pollution across sites and some biological factors on helminth communities infecting the lizard, Chalcides ocellatus. The possibility of heavy metal accumulation by such helminths was also investigated. A total of 202 C. ocellatus were collected from three different sites (industrial, rural, and urban systems) in Ismailia governorate, Egypt, during summer 2009. The lizards were classified according to their sex and size and were examined for the intestinal helminths. Heavy metal levels were detected in the intestinal tissue of the lizards and the recovered helminths. Species richness was 6, 5, and 3 in rural, urban, and industrial systems, respectively. Significant site variations regarding infection prevalence, intensity, and abundance were encountered at different levels. Some noticeable effects of the host size were found. The significant differences found between the metal levels of the intestinal tissues and the recovered helminths and the other relations found in this study may be indications for a possible metals accumulation capacity by helminths. The cestode Oochoristica tuberculata could be a promising biomonitor for Cu and Pb, while the intestinal nematodes were less sensitive to the pollution. Differences in the accumulation capacity may be attributed to the intensity of infection, parasite species, and metal. The observed patterns of distribution and occurrence of helminths and the metals accumulation capacity reflect the need for more studies since this study proposes the model intestinal helminth/C. ocellatus as another promising bioindication system in the terrestrial habitat, especially in areas where the lizard C. ocellatus are available.     

Bio-monitoring for uranium using stream-side terrestrial plants and macrophytes

This study evaluated the abilities of various plant species to act as bio-monitors for environmental uranium (U) contamination. Vegetation and soil samples were collected from a U processing facility. The water-way fed from facility storm and processing effluents was the focal sample site as it represented a primary U transport mechanism. Soils and sediments from areas exposed to contamination possessed U concentrations that averaged 630 mg U kg(-1). Aquatic mosses proved to be exceptional accumulators of U with dry weight (dw) concentrations measuring as high as 12,500 mg U kg(-1) (approximately 1% of the dw mass was attributable to U). The macrophytes (Phragmites communis, Scripus fontinalis and Sagittaria latifolia) were also effective accumulators of U. In general, plant roots possessed higher concentrations of U than associated upper portions of plants. For terrestrial plants, the roots of Impatiens capensis had the highest observed levels of U accumulation (1030 mg kg(-1)), followed by the roots of Cyperus esculentus and Solidago speciosa. The concentration ratio (CR) characterized dry weight (dw) vegetative U levels relative to that in associated dw soil. The plant species that accumulated U at levels in excess of that found in the soil were: P. communis root (CR, 17.4), I. capensis root (CR, 3.1) and S. fontinalis whole plant (CR, 1.4). Seven of the highest ten CR values were found in the roots. Correlations with concentrations of other metals with U were performed, which revealed that U concentrations in the plant were strongly correlated with nickel (Ni) concentrations (correlation: 0.992; r-squared: 0.984). Uranium in plant tissue was also strongly correlated with strontium (Sr) (correlation: 0.948; r-squared: 0.899). Strontium is chemically and physically similar to calcium (Ca) and magnesium (Mg), which were also positively-correlated with U. The correlation with U and these plant nutrient minerals, including iron (Fe), suggests that active uptake mechanisms may influence plant U accumulation.