Excrement from Heron colonies for environmental assessment of toxic elements

Excrement cast from Great Blue Heron nests was collected during the nesting period of 1978 from four colonies in Washington and Idaho. Cheesecloth strips placed on the ground beneath the nests served as excrement collecting devices. Chemical analysis for lead, mercury and cadmium were performed on dried samples. Lead was the most abundant trace metal found in heron debris. The Idaho colony at Lake Chatcolet had an average concentration of 46 ppm in the beneath-nest samples and 6 ppm in control samples. A heron colony near Tacoma, Washington had beneath-nest samples averaging 28 ppm and control samples averaging 20 ppm. Two colonies located in the interior region of Washington had substantially lower concentrations of lead. The difference observed between colonies was attributed to their associations with a polluted watershed (Chatcolet colony) an interstate highway (Tacoma colony) and an unpopulated largely agricultural area (inland Washington).     

Strontium-90 in Canada goose eggshells and reed canary grass from the Columbia River,Washington

Strontium-90 (⁹⁰SR) released to the ground near the N Reactor at the U.S. Department of Energy's Hanford Site enters the Columbia River through shoreline seeps. The ⁹⁰Sr is then potentially available for uptake by plants and animals. The life history and foraging behavior of nesting Canada geese is such that female geese could ingest ⁹⁰Sr while foraging on shoreline plants. Radichemical analyses showed that goose eggshells taken from an island, downstream from the N Reactor, contained more ⁹⁰Sr than did eggshells collected from other downstream islands. Reed canary grass samples taken from shoreline areas immediately downstream from the N Reactor contained higher concentrations of ⁹⁰Sr than samples from other downstream areas. All goose eggshells did not contain enhanced levels of ⁹⁰Sr, and all reed canary grass samples did not contain enhanced levels of ⁹⁰Sr, but a relationship exists between the releases of ⁹⁰Sr to the Columbia River and the enhanced levels of ⁹⁰Sr in some of the environmental samples analyzed.Pacific Northwest Laboratory is operated by Battelle Memorial Institute for the U.S. Department of Energy under contract DE-ACO6-76RLO 1830.     

Environmental monitoring of historical change in arsenic deposition with tree bark pockets

ICP-MS analysis recorded historical change (c. 1846 to 2002) in the arsenic concentration of bark included within the trunks (tree bark pockets) of two Japanese oak trees (Quercus crispula), collected at an elevated location approximately 10 km from the Ashio copper mine and smelter, Japan. The arsenic concentration of the bark pockets was 0.016 +/- 0.003 microg cm(-2) c. 1846 (n = 5) and rose 50-fold from c. 1875 to c. 1925, from approximately 0.01 to 0.5 microg cm(-2). The rise coincided with increased copper production in Ashio from local sulfide ores, from 46 tons per year in 1877 to 16,500 tons per year in 1929. Following a decline in arsenic concentration and copper production, in particular during the Second World War, a second peak was observed c. 1970, corresponding to high levels of production from both local (6,000 tons per year) and imported (30,000 tons per year) ores, smelted from 1954. Compared to the local ores, the contribution of arsenic from imported ores appeared relatively low. Arsenic concentrations declined from c. 1970 to the present following the closure of the mine in 1974 and smelter in 1989, recording 0.058 +/- 0.040 microg cm(-2) arsenic (n = 5) in surface bark collected in 2002. The coincident trends in arsenic concentration and copper production indicated that the bark pockets provided an effective record of historical change in atmospheric arsenic deposition.     

Integration of modeling,monitoring and laboratory observation to determine reasons for air quality violations

An attempt was made to explain ambient air quality violations in the vicinity of industrial plants. Micrometeorology, diffusion calculaations, ambient readings, and plant engineering data are all taken into consideration to ascertain whether the plant is or is not the principal offender.Scrutiny of hi-vol filters using scanning electron microscopy for particle size analysis and particle chemical make-up as an assessment technique is also described.Case histories using the above analytical techniques are discussed.Presented at the 16th National Conference on Energy and the Environment, May 23, 1979     

Ambient air concentrations of PCDDs,PCDFs, coplanar PCBs,and PAHs at the Mississippi Sandhill Crane National Wildlife Refuge,Jackson County,Mississippi

Our objective was to determine the levels of selected airborne contaminants in ambient air at the Mississippi Sandhill Crane National Wildlife Refuge, Mississippi, that might be affecting the health of endangered cranes living there. Two high-volume air samplers were operated at separate locations on the Refuge during May–September 1991. The sampling media were micro-quartz filters in combination with polyurethane foam plugs. Composite bimonthly samples from each station were analyzed for polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), coplanar polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs). Overall, residue concentrations were low. The toxic PCDD isomer 2,3,7,8-tetra-CDD was not detected, nor was penta-CDD. There was no difference (P>0.05) in residue concentrations between stations or over time and meteorological parameters were not correlated with residue concentrations. Because contaminant levels and patterns may differ seasonally, we recommend that air samples collected during winter months also be analyzed for these same chemical groups.     

Mega-Development Trends in the Amazon: Implications for Global Change

This paper describes four global-change phenomena that are having major impacts on Amazonian forests. The first is accelerating deforestation and logging. Despite recent government initiatives to slow forest loss, deforestation rates in Brazilian Amazonia have increased from 1.1 million ha yr^–1 in the early 1990s, to nearly 1.5 million ha yr^–1 from 1992–1994, and to more than 1.9 million ha yr^–1 from 1995–1998. Deforestation is also occurring rapidly in some other parts of the Amazon Basin, such as in Bolivia and Ecuador, while industrialized logging is increasing dramatically in the Guianas and central Amazonia.The second phenomenon is that patterns of forest loss and fragmentation are rapidly changing. In recent decades, large-scale deforestation has mainly occurred in the southern and eastern portions of the Amazon — in the Brazilian states of Pará, Maranho, Rondônia, Acre, and Mato Grosso, and in northern Bolivia. While rates of forest loss remain very high in these areas, the development of major new highways is providing direct conduits into the heart of the Amazon. If future trends follow past patterns, land-hungry settlers and loggers may largely bisect the forests of the Amazon Basin.The third phenomenon is that climatic variability is interacting with human land uses, creating additional impacts on forest ecosystems. The 1997/98 El Niño drought, for example, led to a major increase in forest burning, with wildfires raging out of control in the northern Amazonian state of Roraima and other locations. Logging operations, which create labyrinths of roads and tracks in forsts, are increasing fuel loads, desiccation and ignition sources in forest interiors. Forest fragmentation also increases fire susceptibility by creating dry, fire-prone forest edges.Finally, recent evidence suggests that intact Amazonian forests are a globally significant carbon sink, quite possibly caused by higher forest growth rates in response to increasing atmospheric CO₂ fertilization. Evidence for a carbon sink comes from long-term forest mensuration plots, from whole-forest studies of carbon flux and from investigations of atmospheric CO₂ and oxygen isotopes. Unfortunately, intact Amazonian forests are rapidly diminishing. Hence, not only is the destruction of these forests a major source of greenhouse gases, but it is reducing their intrinsic capacity to help buffer the rapid anthropogenic rise in CO₂.     

Sampling Benthic Macroinvertebrates in a Large Flood-Plain River: Considerations of Study Design, Sample Size, and Cost

Estimation of benthic macroinvertebrate populations over large spatial scales is difficult due to the high variability in abundance and the cost of sample processing and taxonomic analysis. To determine a cost-effective, statistically powerful sample design, we conducted an exploratory study of the spatial variation of benthic macroinvertebrates in a 37 km reach of the Upper Mississippi River. We sampled benthos at 36 sites within each of two strata, contiguous backwater and channel border. Three standard ponar (525 cm²) grab samples were obtained at each site ('Original Design'). Analysis of variance and sampling cost of strata-wide estimates for abundance of Oligochaeta, Chironomidae, and total invertebrates showed that only one ponar sample per site ('Reduced Design') yielded essentially the same abundance estimates as the Original Design, while reducing the overall cost by 63%. A posteriori statistical power analysis ( = 0.05, = 0.20) on the Reduced Design estimated that at least 18 sites per stratum were needed to detect differences in mean abundance between contiguous backwater and channel border areas for Oligochaeta, Chironomidae, and total invertebrates. Statistical power was nearly identical for the three taxonomic groups. The abundances of several taxa of concern (e.g., Hexagenia mayflies and Musculium fingernail clams) were too spatially variable to estimate power with our method. Resampling simulations indicated that to achieve adequate sampling precision for Oligochaeta, at least 36 sample sites per stratum would be required, whereas a sampling precision of 0.2 would not be attained with any sample size for Hexagenia in channel border areas, or Chironomidae and Musculium in both strata given the variance structure of the original samples. Community-wide diversity indices (Brillouin and 1-Simpsons) increased as sample area per site increased. The backwater area had higher diversity than the channel border area. The number of sampling sites required to sample benthic macroinvertebrates during our sampling period depended on the study objective and ranged from 18 to more than 40 sites per stratum. No single sampling regime would efficiently and adequately sample all components of the macroinvertebrate community.     

Monitoring of diazinon concentrations and loadings,and identification of geographic origins consequent to stormwater runoff from orchards in the Sacramento River watershed,U.S.A

Diazinon contamination of California's rivers has resulted in placing several rivers on the federal Clean Water Act § 303d list of impaired waterways. Impaired water body listing requiresthe development of Total Maximum Daily Loads (TMDL). Previous studies identified stormwater related diazinon pulses in California rivers. This study was conducted to monitor diazinonconcentrations in the Sacramento River watershed after rainfallevents, to ascertain whether pulses could be identified and, ifconcentrations of concern were observed, to estimate loadings anddetermine geographic origins of the insecticide. TMDL developmentrequires knowledge of contaminant sources, loadings, and geographic origins. Flow and diazinon concentrations peaked in the Sacramento River at Sacramento after the three largest stormsduring January and February 1994. Diazinon concentrations peakedconsequent to each of three storms. Diazinon concentrationsmeasured in the Sacramento River at Sacramento exceeded theCalifornia Department of Fish and Game acute and chroniccriteria for protection of aquatic life during January andFebruary for nine and nineteen days, respectively. Multipleexceedances were observed throughout the watershed. Diazinon loading and geographic origin differed with each of the three storms. The design of this study provides a useful template for others attempting to identify loadings and sources of contaminants in surface waters and to rectify aquatic ecosystemcontamination from various land use practices.     

Determining Ecoregions for Environmental and GMO Monitoring Networks

A representative environmental monitoring network at the regional scale cannot use raster-based or random sampling designs, but requires a stratified sampling procedure integrating different information layers, and it has to occur in ecologically differing homogeneous regions (ecoregions). These we have determined using a set of spatial strata with ecological variables which we analysed with classification and regression trees (CART). We present a framework for environmental monitoring, that covers different scales, and we transfer the framework to a potential GMO (genetically modified organisms) monitoring network. We use ecoregion and other environmental strata together with existing environmental monitoring networks to determine GMO monitoring sites more precisely.     

Arsenic compounds in the haemolymph of the Dungeness crab, Cancer magister, as determined by using HPLC on-line with inductively coupled plasma mass spectrometry

Arsenobetaine, two arsenosugars, dimethylarsinate and several unidentified arsenic species were detected in extracts of the haemolymph of the Dungeness crab, Cancer magister, by using HPLC-ICP-MS. This is the first report of the presence of arsenosugars in the haemolymph/blood of marine animals. Total, extractable and residual arsenic concentrations were determined by ICP-MS. The concentration of total arsenic was in the range of 1.4-3.8 [micro sign]g ml(-1). Nearly all (98%) the arsenic was found to be extractable, and accounted for primarily by arsenobetaine, two arsenosugars and dimethylarsinate. The results demonstrate that arsenic compounds present in the diet of crabs are not fully metabolized in the gut. They are, at least partly, taken up into the haemolymph. The concurrence of arsenobetaine and arsenosugars suggests that the use of repeated haemolymph sampling in crustaceans could facilitate investigations into the kinetics of the biotransformation pathways of arsenic compounds. Finally, the present study clearly demonstrates the unique capabilities of HPLC-ICP-MS for the detection and identification of minor arsenic components amongst the predominant arsenobetaine.