焦化废水生物膜样品和苯酚降解菌分离株基因盒的分析

使用59-碱基(59be)保守序列和整合酶基因的引物进行PCR扩增,研究了焦化废水处理装置中微生物菌群的基因盒结构,并对两株分离的苯酚降解荫IS-17和IS-46的基因盒进行了扩增测序分析.从5个焦化废水生物膜总DNA样品中均获得了基因盒扩增产物.对IS-17和IS-46的基因盒PCR产物克隆建库并测序,通过序列比对等方法来研究和分析序列的功能.发现本研究获得的基因盒与已报道的基因盒存读码框序列、两端引物结合序列、间隔区大小等方面存在一些不同.本研究结果表明,在焦化废水生物膜菌群中普遍存在基因盒结构,采用基因盒PCR能够从环境或菌株中获取新基因.     

Removal of high concentrations of polycyclic aromatic hydrocarbons from contaminated soil by biodiesel

Solubilizing experiments were carried out to evaluate the ability of biodiesel to remove polycyclic aromatic hydrocarbons (PAHs) from highly contaminated manufactured gas plant (MGP) and PAHs spiked soils with hydroxypropyl-β-cyclodextrin (HPCD) and tween 80 as comparisons. Biodiesel displayed the highest solubilities of phenanthrene (420.7 mg·L^-1), pyrene (541.0 mg·L^-1), and benzo(a)pyrene (436.3 mg·L^-1). These corresponded to several fold increases relative to 10% HPCD and tween 80. Biodiesel showed a good efficiency for PAH removal from the spiked and MGP soils for both low molecular weight and high molecular weight PAHs at high concentrations. Biodiesel was the best agent for PAH removal from the spiked soils as compared with HPCD and tween 80; as over 77.9% of individual PAH were removed by biodiesel. Tween 80 also showed comparable capability with biodiesel for PAH solubilization at a concentration of 10% for the spiked soils. Biodiesel solubilized a wider range of PAHs as compared to HPCD and tween 80 for the MPG soils. At PAH concentrations of 229.6 and 996.9 mg·kg^-1, biodiesel showed obvious advantage over the 10% HPCD and tween 80, because it removed higher than 80% of total PAH. In this study, a significant difference between PAH removals from the spiked and field MGP soils was observed; PAH removals from the MGP soil by HPCD and tween 80 were much lower than those from the spiked soil. These results demonstrate that the potential for utilizing biodiesel for remediation of highly PAH-contaminated soil has been established.     

农药含钾废渣的热解动力学

通过XRF和XRD两种测试方法对农药含钾废渣的成分进行测定,结果显示,该废渣中含有大量的氯化钾,具有很大的资源化价值.采用热重分析法,在5、10、15、20 K.min-1线性升温速率下,研究了农药含钾废渣在空气气氛下的热解特性及热解动力学.以期为农药含钾废渣的资源化处理提供理论依据.结果表明,该废渣热解过程分为3个阶段,失重率分别为4.62%、7.69%和2.55%;且3个阶段的活化能E分别为83.53、128.48和138.17 kJ.mol-1,指前因子A分别为4.79×107、2.34×1010和6.46×107s-1.     

一株中度嗜盐菌的分离及生物学特性

从我国内蒙古吉兰泰盐湖的样品中分离得到一株中度嗜盐细菌菌株JLT-01,研究了其形态和特性.结果显示:该菌株为杆状,革兰氏阴性,无芽孢,无荚膜,无鞭毛;最适培养温度为30℃,最适生长pH值7.5,最适生长盐度10%~12%(m/V),最适生长Mg2+浓度3.0%(m/V);能利用葡萄糖、蔗糖、半乳糖作为唯一碳源生长.脂肪酸成分分析表明在JLT-01中主要脂肪酸为C16:0与C18:1ω9c,含量分别为26.39%和20.30%.Tm法测定该菌的(G+C)含量(摩尔分数)为52.3%.以该菌的16S rRNA基因序列为基础构建了系统发育树;16S rRNA基因序列的序列同源性比对表明该菌与海杆菌属Marinobacter lipolyticus SM19T的同源性为98.0%,与Marinobacter属内其它菌株的同源性在96.0%~99.0%之间.DNA-DNA杂交实验分析显示:JLT-01与M.lipolyticus DSM15157的杂交率小于70%.结果表明,菌株JLT-01是海杆菌属的一个新菌株.     

人因可靠性分析的加权CREAM模型

为有效预测开发阶段系统的可靠性,提高认知可靠性与失误分析方法(CREAM)分析的准确性,降低部分系统输入数据的主观性,利用区间值直觉模糊集(IVIFS)和网络分析法(ANP),在考虑共同绩效条件(CPC)间相关性的情况下,提出了一种定量计算CPC权重的优化方法,使可靠性分析更加符合实际环境.在算法中基于判断矩阵获得各CPC权重,克服了一致性检验的约束,并确定了加权的人因失效概率.最后将改进前后的方法应用在肺癌穿刺机器人手术系统的试验中.结果表明:改进后方法获得的权重的标准误差(SE)更低,与真实值更贴近,准确性更高,证明了其分析能力的优越性;改进后方法最终预测结果包含的信息更广,有效避免了对系统潜在故障的忽视.     

不同墩高下连续刚构桥抗震性能分析

以某大跨连续高墩刚构桥为依托,采用Midas/civil软件建立仿真模型,通过时程分析方法研究双肢薄壁墩参数在地震响应下对高墩连续刚构桥抗震性能的影响.以桥墩高度为主要参数,以模型阵型频率、桥墩墩顶、墩底位移及内力变化三个方面对其抗震性能进行评价.研究结果表明,随着其中一个桥墩高度的增加,桥梁频率不断减小,矮墩墩底及矮...     

江苏省农村土地征用与收益分配研究

本文立足江苏省的土地征用和土地收益分配现实，指出目前存在“圈地”现象严重、征地补偿过低、失地农民未来生活无保障等问题。从政府、制度、城市建设三个层面探讨了问题产生的根源，并提出了提高土地补偿标准、建立失地农民保障体系、广开就业门路、健全法律体系等改革措施。     

A Radio Communication System for Neuronal Signals

Abstract

To collect neuronal activity data from awake, freely behaving animals, we developed miniature telemetry recording system. The integrated system consists of four major components: 1) Microelectrodes and micro-driver assembly, 2) analog front end (AFE), 3) programmable system on chip (PSoC), and 4) radio transceiver and the LabVIEW were used as a platform for the graphic user interface. The result showed the system was able to record and analyze neuronal recordings in freely moving animals and lasted continuously for a time period of a week or more. This is very useful for the study of the interdisciplinary research of neuroscience and information engineering techniques. The circuits and architecture of the devices can be adapted for neurobiology and research with other small animals.     

基于星座图法的湖北省农业功能分区

农业功能分区是准确把握县域农业功能定位、推动区域农业功能拓展和向现代农业转型的基础工作。旨在探索运用一种新的分类方法即星座图法在农业功能分区中的应用。剖析现代农业的功能定位问题,从农产品供应、就业与社会保障、生态调节和文化传承与休闲4大类综合功能选取30个定量指标和2个定性指标,构建了湖北省农业功能分区指标体系。采用星座图法,根据星座图中各星点的位置,确定各县（市）单项功能的分等类型;并以此为基础结合分区单元的实际地理位置和社会经济及环境现状,作出湖北省农业功能分区方案。分区结果以县级行政区域为分区单元,按照农业功能分区的基本原则,湖北省分4个农业主导功能区,各主导功能区分为3个二级区共12个二级农业功能区