Oxidation of Cyclic Ethers by Alkane‐Grown Mycobacterium vaccae JOB5

In this study we investigated the cometabolic oxidation of six cyclic ethers by alkane‐grown Mycobacterium vaccae JOB5. These ethers include, among others, tetrahydrofuran (THF), 1,4‐dioxane (14D), 1,3‐dioxolane (13DO), and tetrahydropyran (THP). Cells grown on propane, n‐butane, n‐pentane, isobutane, or isopentane oxidized all six ethers. Ether‐degrading activity was inhibited by acetylene in alkane‐grown cells and was largely absent from cells grown on dextrose‐containing media. Propane competitively inhibited THF oxidation. γ‐Butyrolactone (γBL) accumulated and was also further oxidized during THF oxidation by propane‐grown cells. In contrast, no products were detected during 14D oxidation. Propane‐grown cells also rapidly oxidized 3‐hydroxytertrahydrofuran and exhibited strong hemiacetal‐oxidizing activity in an assay following methyl formate production from mixtures of methanol and formaldehyde. These observations suggest γBL is likely generated during THF oxidation through further oxidation of 2‐hydroxytetrahydrofuran. Limited growth of strain JOB5 was supported by several cyclic ethers and the corresponding lactones and diols potentially derived from these compounds. However, strain JOB5 grew more readily on 4‐hydroxybutyrate, the product of γBL hydrolysis. The ability of strain JOB5 to productively assimilate THF‐derived metabolites during growth on n‐alkanes was examined in carbon‐limited batch cultures. Relative to C‐limited growth on n‐pentane alone, culture growth increased up to twofold in the presence of THF while no stimulation of growth was observed in comparable experiments conducted with n‐pentane and 14D. Our results are discussed in terms of their significance to our understanding of cyclic ether cometabolism and their potential impact on approaches for cyclic ether biodegradation in the environment. © 2013 Wiley Periodicals, Inc.     

新安江水库水环境主要问题及保护对策

新安江水库是钱塘江的重要水源,对保障钱塘江中下游的生态安全和水体功能起着举足轻重的作用。为阐明新安江水库在流域经济发展和人类活动的影响下水环境的主要问题及保护对策,促进饮用水安全保障及构建健康水域生态系统,本文对新安江水库历年的水环境指标、浮游生物群落结构及其变化及水域生态灾变事件资料进行了综合分析。目前新安江水库存在上游来水变差、局部水域藻类异常增殖、渔业生产不合理、营养程度加重等众多问题,但其突出问题是藻类生物量增长过快。新安江水库水环境保护工作要从关注水质向维持生态系统健康转变,并尽快开展生态保育工作,控制流域污染、降低藻类数量、减少藻类异常增殖影响、科学合理渔业生产是其下一步保护的关键。而关于新安江水库的研究,虽然有一定的研究基础和资料,但对水域生态系统缺乏系统认识。研究气候变化条件下新安江水库水域生态系统的结构和功能以及长期演变规律;水利调度导致水位调整对区域水动力学条件、营养物输送过程的影响及评价水利调度对水库重要环境因子和生态系统影响;渔业养殖对水环境的影响,特别是鲢、鳙鱼大水面积养殖对水库生态系统的影响应是今后新安江水库主要的研究领域和方向。     

Roles of Scholars in the Practice of Combating-Desertification: A Case Study in Northwest China

This study investigated the perceived importance of scholars’ participation in combating-desertification programs in northwest China and analyzed the underlying factors and mechanisms. Our results show that, while various experts, professors, and researchers have participated in combating-desertification programs, their actions were often not effective. Only those scholars who understood the local situations adequately had important and positive impacts. These scholars served as information brokers between the governments and other stakeholders, entrepreneurial activity organizers for farmers, governmental representatives, or advocators for local affairs themselves. They played indispensible roles in facilitating efforts in combating desertification. The study also identified key factors that led to the success of scholars’ participation in combating-desertification activities. Our findings have practical implications for improving the effectiveness of scholars’ participation in land restoration and environmental management.     

Pentachlorophenol induced physiological-biochemical changes in Chlorella pyrenoidosa culture

Present study investigated physiological and biochemical changes in a green alga culture (Chlorella pyrenoidosa) upon exposure to varied concentrations of pentachlorophenol (PCP) (0, 0.4 mgL(-1), 2.5 mgL(-1) and 10 mgL(-1)). The results revealed that with the increase of PCP concentration, a decrease of the algal biomass, levels of photosynthetic pigments (chlorophyll a, b and carotenoid), soluble protein and an increase of nitrate reductase (NR) activity were observed. This suggests that the PCP may serve as an uncoupler, causing low ATP level within the algal cells and led most of the NR molecules to be in the dephosphoration state (i.e. active from). Moreover, it shows that photosynthetic pigments especially carotenoid were more sensitive indicators to indicate PCP toxicity as compared to the other parameters.     

固定化解脂耶氏酵母间歇式处理油脂废水研究

固定化解脂耶氏酵母菌(Yarrowia lipolytica)在气升式反应器中处理色拉油浓度为2 000 mg/L、COD为3 000 mg/L的高COD油脂废水。结果表明,固定化菌对废水的最佳处理时间为6 d,可间歇式处理9批次高COD油脂废水,重复处理废水后,其对油脂的降解均在85%以上,对COD的降解在70%以上,降解后的排出水pH稳定在3.6~3.8。且处理动力学方程属于一级生化方程。结果进一步表明,固定化解脂耶氏酵母菌在气升式反应器中适于处理高COD含油脂的污水。     

鄱阳湖典型湿地土壤有机质及氮素空间分布特征

通过对鄱阳湖三个典型湿地植物群落带土壤有机质和全氮的空间分布特征的研究,结果表明：有机质和全氮在湿地土壤中的垂直分布规律具有一致性,表层含量显著高于下层,40 cm以下土壤养分含量基本稳定。表层土壤有机质和全氮含量不同植物群落间存在差异,有机质变化趋势为：刚毛荸荠群落带＜南荻群落带＜灰化苔草群落带;氮素含量的变化趋势为：南荻群落带＞灰化苔草群落带＞刚毛荸荠群落带。土壤碳氮比相对较低（10～23）;植物对养分的吸收能力、植被生长特征、湿地生态水文过程等影响着有机质和氮素含量的空间分布。
     

堆码试验标准差异的探讨与应用

本文介绍GJB 2711-1996中方法 13、方法 14两种堆码试验方法的异同点,以及它们与现行国家标准GB/T 4857.3-2008及GB/T 4857.4-2008中两种堆码试验方法的比较,说明堆码试验方法在国军标与国标中的共同点和差异,以及堆码试验中需要关注的问题。     

3×300t转炉二次除尘系统扩容改造

针对宝钢一炼钢3×300t转炉二次烟气严重污染室内外环境的实际情况,在兼顾节能与减排的协同原则下,确定了对原转炉二次除尘系统的扩容改造方案。竣工投运后,取得了良好的环保、节能效果。     

鄱阳湖老爷庙水域细菌群落组成分析

通过提取鄱阳湖老爷庙水域水体细菌总DNA, 构建细菌16SrRNA基因文库和序列测定等手段,以期揭示鄱阳湖老爷庙水域的细菌群落组成并探讨细菌多样性与湖泊水体理化参数之间的关系。研究结果认为：来自老爷庙水域的2006年10月（PN06 10）和2007年5月 (PN07 5) 的细菌文库中的180个阳性克隆产生了66个细菌分类操作单位（OTUs）,这些细菌主要分属于：变形菌Alpha 纲,Beta 纲,Gamma 纲,Epsilon 纲,拟杆菌门,放线菌门,浮霉菌门,酸杆菌门和OP10等9大类型。2006年10月老爷庙水域的优势菌群为Beta 变形菌;2007年5月优势菌群为Gamma 变形菌和拟杆菌,表明鄱阳湖细菌组成在不同的季节显著不同。进一步系统发育分析表明：大部分细菌的最相似序列来源于淡水,湖泊和土壤的未培养细菌,且部分细菌与环境污染物降解有关