Role of cosubstrate and bioaccessibility played in the enhanced anaerobic biodegradation of organochlorine pesticides (OCPs) in a paddy soil by nitrate and methyl-β-cyclodextrin amendments

The present study was conducted to investigate the anaerobic biodegradation potential of biostimulation by nitrate (KNO₃) and methyl-β-cyclodextrin (MCD) addition on an aged organochlorine pesticide (OCP)-contaminated paddy soil. After 180 days of incubation, total OCP biodegradation was highest in soil receiving the addition of nitrate and MCD simultaneously and then followed by nitrate addition, MCD addition, and control. The highest biodegradation of chlordanes, hexachlorocyclohexanes, endosulfans, and total OCPs was 74.3, 63.5, 51.2, and 65.1 %, respectively. Meanwhile, MCD addition significantly increased OCP bioaccessibility (p?<?0.05) evaluated by Tenax TA extraction and a three-compartment model method. Moreover, the addition of nitrate and MCD also obtained the highest values of soil microbial activities, including soil microbial biomass carbon and nitrogen, ATP production, denitrifying bacteria count, and nitrate reductase activity. Such similar trend between OCP biodegradation and soil-denitrifying activities suggests a close relationship between OCP biodegradation and N cycling and the indirect/direct involvement of soil microorganisms, especially denitrifying microorganisms in the anaerobic biodegradation of OCPs.     

Characterization of a fenpropathrin-degrading strain and construction of a genetically engineered microorganism for simultaneous degradation of methyl parathion and fenpropathrin

A gram-negative fenpropathrin-degrading bacterial strain Sphingobium sp. JQL4-5 was isolated from the wastewater treatment sludge of an insecticide factory. Strain JQL4-5 showed the ability to degrade other pyrethroid insecticides, but it was not able to degrade methyl parathion. To enhance its degrading range of substrate, a methyl parathion hydrolase gene (mpd) was successfully introduced into the chromosome of strain JQL4-5 with a mini-Tn-transposon system. A genetically engineered microorganism (GEM) named JQL4-5-mpd resulted, which was capable of simultaneously degrading methyl parathion and fenpropathrin. Soil treatment results indicated that JQL4-5-mpd is a promising multifunctional bacterium in the bioremediation of multiple pesticide-contaminated environments.     

Effects of leachate accumulation on landfill stability in humid regions of China

Leachate levels are important to landfill stability and safety. High leachate or water levels often lead to landfill instability, which can cause accidents. Here a case study of a landfill located in a humid region of southern China is presented. Leachate distribution and quality were systematically analyzed, and the effect of leachate level on waste-mass stability was assessed. Boreholes were drilled in the field, samples were analyzed in the laboratory, and a simulation was performed. In addition, the safety and stability of the landfill was evaluated. The leachate level in the landfill was 9–19 m, which was higher than the top of the dam crest (8–20 m). Leachate accounted for more than 1/4 of the total landfill storage capacity. The contaminant concentration of the leachate samples collected directly from the waste body was very high, with large variation among the samples. The mean concentrations of NH₃–N, BOD, and COD from the waste body were 5404, 14,136, and 22,691 mg/L, nearly 2.7, 2.4, and 1.8 times the mean concentrations in the leachate pond, respectively. Three series of shear strength parameters were used in a slope stability analysis, and a limit equilibrium method was used to calculate the factor of safety (Fs). The analysis showed that Fs could be affected by potential anisotropy in the shear strength of the waste. The minimum values of Fs corresponding to series I were 1.84 and 1.17 for units ? and II, respectively. The Fs value of unit II was significantly lower than the safe design value (1.25). In addition, Fs decreased with increase in the normalized height of the leachate level, h/H, where h is the height of the leachate mound and H is the maximum thickness of the landfill. If the h/H values of units I and II are kept below 50% and 40%, respectively, a safe design value of 1.25 for Fs can be guaranteed. Therefore, some measures to prevent risk should be considered.     

没食子酸生产废炭渣的处理

采用热水洗涤法回收没食子酸生产废炭渣中的没食子酸,过滤炭渣经热再生处理得到热再生活性炭。研究了热水洗涤法回收没食子酸和热再生法再生活性炭的最佳工艺条件。实验结果表明,热水洗涤法回收没食子酸的优化工艺条件:废炭渣质量与热水体积比为0.15g/mL,热水洗涤时间为60min,热水洗涤温度为60℃。在此最佳工艺条件下,没食子酸回收量达52mg/g。热再生法再生活性炭的最佳工艺条件为:热再生温度500℃,热再生时间120min。在此条件下制备的热再生活性炭对亚甲基蓝的吸附量达168mg/g,热再生活性炭平均得率为57.1%。     

加快攀西-六盘水地区优势资源的开发

攀西-六盘水地区水能、矿产资源丰富,是建设我国能源、原材料生产的重要基地,也是长江上游资源开发和生态保护区的重要组成部分。本文在分析加快开发该区优势资源的有利条件和不利因素的基础上,提出了开发建设的总体目际和思路,突出水能和矿产资源优势,加强对农业和林业的开发,力争到21世纪中叶使该区建成我国重要的能源、原材料生产基地,从根本上改变该区文通闭塞、经济、文化落后的面貌,在主要经济、技术指标上赶上全国的平均先进水平。     

四川酸性物湿沉降模式及预测

酸沉降是一复杂的大气物理和化学过程，涉及诸多复杂影响因素。目前，我国酸雨湿沉降模式还仅限于模拟云下洗脱成酸过程，而对云中的成酸过程还未进行深入研究，这不仅在理论上不完善，而且更重要是在许多情况下与我省实际情况不符。本课题针对四川省特定的地理，气象特点和严重的大气污染情况，并借鉴国外研究成果，提出了考虑酸性沉降的云中和云下成酸过程和各种影响因素的综合酸雨湿沉降模式，并根据此模式来估算和预测四川省酸雨     

城市防洪工程风险决策方法

本文着重讨论了与城市防洪工程决策制定密切相关的几个问题：①城市防洪工程设计标准的确定；②现有防洪工程的性能评估，③最优防洪工程对策的确定等，并在风险分析的基础上，给出了解决上述问题的方法。所得结论可为城市防洪工程决策的制定提供较为科学的理论依据。     

“暖冬”对农业生产及农业灾害的影响

本文分析了江苏省近几十年来、特别是８０年代以来冬季气温的变化特征，根据农业灾害发生的条件，讨论了“暖冬”对农业生产及农业灾害的影响，为农业生产部门和防灾抗灾部门决策提供科学依据．     

利用煮茧废水培养钝顶螺旋藻的初步研究

本文对缫丝厂煮茧废水和Ｚａｒｒｒｏｕｋ培养基的主要营养成份进行了分析比较，筛选出了适合钝顶螺旋藻（Ｓｐｉｒｕｌｉｎａｐｌａｔｅｎｓｉｓ）在煮茧废水中生长繁殖的培养基和与之相适应的驯化藻种。经室外１５０升煮茧废水扩大培养，干藻生物量可达１６．０３克／米￣２·天，粗蛋白含量达５８．６％，含有１７种氨基酸。比用Ｚａｒｒｏｕｋ培养基培养的螺旋藻产量提高１５．７８％，成本减少８０％以上。培养后的煮茧废水ＣＯＤ和ＢＯＤ＿５浓度低于我国污水综合排放标准。研究结果表明：采用本实验室筛选的煮茧废水培养基和驯化藻种，在煮茧废水中大量培养螺旋藻，实现废水资源化是可行的，具有明显的经济效益和环境效益。     

REVIEW ON GLOBAL NATURAL DISASTERS IN 2000

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