Biodiversity mediates productivity through different mechanisms at adjacent trophic levels

Biodiversity may enhance productivity either because diverse communities more often contain productive species (selection effects) or because they show greater complementarity in resource use. Our understanding of how these effects influence community production comes almost entirely from studies of plants. To test whether previous results apply to higher trophic levels, we first used simulations to derive expected contributions of selection and complementarity to production in competitive assemblages defined by either neutral interactions, dominance, or a trade-off between growth and competitive ability. The three types of simulated assemblages exhibited distinct interaction signatures when diversity effects were partitioned into selection and complementarity components. We then compared these signatures to those of experimental marine communities. Diversity influenced production in fundamentally different ways in assemblages of macroalgae, characterized by growth-competition trade-offs, vs. in herbivores, characterized by dominance. Forecasting the effects of changing biodiversity in multitrophic ecosystems will require recognizing that the mechanism by which diversity influences functioning can vary among trophic levels in the same food web.     

Immune defense and reproductive pace of life in Peromyscus mice

Immune activity is variable within and among vertebrates despite the potentially large fitness costs of pathogens to their hosts. From the perspective of life history theory, immunological variability may be the consequence of counterbalancing investments in immune defense against other expensive physiological processes, namely, reproduction. In the present study, we tested the hypothesis that immune defense among captive-bred, disease-free Peromyscus mice would be influenced by their reproductive life history strategies. Specifically, we expected that small species that reproduce prolifically and mature rapidly (i.e., fast pace of life) would favor inexpensive, nonspecific immune defenses to promote reproductive proclivity. Alternatively, we expected that large species that mature slowly and invest modestly in reproduction over multiple events (i.e., slow pace of life) would favor developmentally expensive, specific immune defenses and avoid cheap, nonspecific ones because such defenses are predisposed to self-damage. We found that species exhibited either strong ability to kill (gram-negative) bacteria, a developmentally inexpensive defense, or strong ability to produce antibodies against a novel protein, a developmentally expensive defense, but not both. Cell-mediated inflammation also varied significantly among species, but in a unique fashion relative to bacteria killing or antibody production; wound healing was comparatively similar among species. These results indicate that Peromyscus species use immune strategies that are constrained to a dominant axis, but this axis is not determined solely by reproductive pace of life. Further comparisons, ideally with broader phylogenetic coverage, could identify what ecological and evolutionary forces produce the pattern we detected. Importantly, our study indicates that species may not be differentially immunocompetent; rather, they use unique defense strategies to prevent infection.     

Genomewide analysis indicates that queen larvae have lower methylation levels in the honey bee (Apis mellifera)

The honey bee is a social insect characterized by caste differentiation, by which a young larva can develop into either a queen or a worker. Despite possessing the same genome, queen and workers display marked differences in reproductive capacity, physiology, and behavior. Recent studies have shown that DNA methylation plays important roles in caste differentiation. To further explore the roles of DNA methylation in this process, we analyzed DNA methylome profiles of both queen larvae (QL) and worker larvae (WL) of different ages (2, 4, and 6 day old), by using methylated DNA immunoprecipitation-sequencing (meDIP-seq) technique. The global DNA methylation levels varied between the larvae of two castes. DNA methylation increased from 2-day- to 4-day-old QL and then decreased in 6-day-old larvae. In WL, methylation levels increased with age. The methylcytosines in both larvae were enriched in introns, followed by coding sequence (CDS) regions, CpG islands, 2 kbp downstream and upstream of genes, and 5′ and 3′ untranslated regions (UTRs). The number of differentially methylated genes (DMGs) in 2-, 4-, and 6-day-old QL and WL was 725, 3,013, and 5,049, respectively. Compared to 4- and 6-day-old WL, a large number of genes in QL were downmethylated, which were involved in many processes including development, reproduction, and metabolic regulation. In addition, some DMGs were concerned with caste differentiation.     

Survival and activity of the oyster drill Urosalpinx cinerea under conditions of fluctuating salinity

The effects of fluctuating salinities on the survival and activity of Urosalpinx cinerea (Say) from the James River, Virginia, USA, were observed and compared to results of similar studies at constant salinities. All experiments were conducted at summer temperatures. The lower extremes of salinity fluctuations, especially values below 9, had the greatest effect on mortality; the upper extremes may have delayed, but did not reduce, mortality. There was a characteristic 10-day mortality-free period at the start of the fluctuating salinity experiments. Mortality patterns were much different in constant salinities, where the first 2 weeks of exposure were characterized by highest mortalities. In both types of experiments, drill activity, measured by attachment, feeding, and oviposition, increased as salinities increased above lethal levels (greater than a minimum of 9). Fluctuating salinities, which approximated field conditions, affected drills differently from constant salinities and were, therefore, more realistic for study of these estuarine organisms.Contribution No. 543, Virginia Institute of Marine Science, submitted by the senior author to the School of Marine Science of the College of Williams and Mary, in partial fulfillment of the requirements for the Master of Arts degree.     

Earthworm assisted bioremediation of organic contaminants

Due to their biological, chemical and physical actions, earthworms can be directly employed within bioremediation strategies to promote biodegradation of organic contaminants. Earthworms have been shown to aerate and bioturbate soils and improve their nutritional status and fertility, which are variables known to limit bioremediation. Earthworms have also been shown to retard the binding of organic contaminants to soils, release previously soil-bound contaminants for subsequent degradation, and promote and disperse organic contaminant degrading microorganisms. This review discusses these earthworm actions upon the soil environment and how they might influence the fate and behaviour of soil associated organic contaminants, subsequently improving bioremediation potential. The latter part of this review considers organic compounds in the following order: agrochemicals, petroleum and crude oil hydrocarbons, PAHs and PCBs.     

Fathead Minnow and Bluegill Sunfish Life‐Stage Responses to 17β‐Estradiol Exposure in Outdoor Mesocosms

Developmental and reproductive effects of 17β‐estradiol (E2) exposure on two generations of fathead minnows and one generation of bluegill sunfish were assessed. Fish were exposed to E2 for six continuous weeks in outdoor mesocosms simulating natural lake environments. First generation fish were exposed while sexually mature. Second generation fathead minnows were exposed either during early development, sexual maturity, or both stages. Multiple endpoints were measured to assess effects of E2 exposure on fecundity and fish health and development. Plasma vitellogenin concentrations were highly variable in all fish. Differences in egg production timing for both species indicate differences in fecundity between females exposed to E2 and controls. First generation fathead minnows exposed to E2 had lower body condition factors and reduced secondary sexual characteristic expression by males. Only a difference in relative liver weight was observed in second generation fathead minnows. First generation bluegill males exposed to E2 had significantly smaller testes compared to controls. Although fish response was highly variable, results indicate that exposure to E2 at environmentally relevant concentrations affect fathead minnow and bluegill sunfish health and development, which may have implications for the health and sustainability of fish populations. Furthermore, exposure timing and environmental factors affect fish response to E2 exposure.     

联合基于野外的代谢组学和化学分析手段筛选优先关注新兴污染物：在五大湖流域的案例研究

由于毒性评估项目很难与日渐增长的需要测试的污染物保持同步,所以较难将关注点集中在影响水生生态系统的最为生物相关的污染物上。由于评估潜在毒性污染物所造成的生物影响已被证明是有效的,内生性代谢物的研究（代谢组学）对于剔除那些较低可能造成生物影响的污染物或许有一定帮助,从而找出生物重要性最高的污染物。本研究在北美五大湖流域的18个地点针对置于笼中的黑头软口鲦（Pimephales promelas）进行实验。我们测定了水体温度和水样中的污染物浓度（目标污染物132种,检出86种）,并使用1H-NMR谱测量了肝极性提取物中的内生性代谢物。利用偏最小二乘法回归来比对内生性代谢物的相对丰度与污染物浓度和环境温度。结果表明内生性极性代谢物的指标与最多49种污染物存在共同变化。因此我们认为至多52%的检出污染物与内生性代谢物变化的共同变化不显著,表明这些污染物很可能不会在这些地点造成可以检测到的影响。这是通过缩短对于实验地点有着潜在影响的污染物列表从而扫描出检出污染物生物相关性的第一步。类似的信息有助于风险评估者区分不同污染物的重要性并将重点毒性测试放在最为生物相关的污染物上。
精选自Nicol Janecko, Lucie Pokludova, Jana Blahova, Zdenka Svobodova, Ivan Literak. Linking field-based metabolomics and chemical analyses to prioritize contaminants of emerging concern in the Great Lakes basin. Environmental Toxicology and Chemistry: Volume 35, Issue 10, pages 2493–2502, October 2016. DOI: 10.1002/etc.3409
详情请见http://onlinelibrary.wiley.com/doi/10.1002/etc.3409/full
     

Socially acquired predator recognition in complex ecosystems

Social animals acquire information on predator identities through social learning, where individuals with no prior experience learn from experienced members of the group. However, a large amount of uncertainty is often associated with socially acquired information especially in cases of cross-species learning. Theory predicts that socially acquired information from heterospecifics should take more repetitions to develop in complex ecosystems where the number of participants is greater. Our work focuses on coral reef fish as their social and communal lifestyles, along with their complex life histories, make them an ideal model to test for socially acquired predator recognition. Specifically, we tested if Pomacentrus wardi were capable of transmitting the recognition of an unknown predator, Pseudochromis fuscus, to closely related Pomacentrus moluccensis and phylogenetically distant Apogon trimaculatus. Individuals of both species were able to learn the predator's identity from experienced P. wardi based on a single conditioning event. It is somewhat surprising how fast social learning occurred particularly for the distantly related cardinalfish. This study demonstrates the widespread nature of social learning as a method of predator recognition in biologically complex ecosystems, and highlights that the benefits of responding to uncertain information may override the costs associated with lost foraging opportunities.     

Direct and Indirect Effects of Forest Harvesting on Sediment Yield in Forested Watersheds of the United States

Managed forests generally produce high water quality, but degradation is possible via sedimentation if proper management is not implemented during forest harvesting. To mitigate harvesting effects on total watershed sediment yield, it is necessary to understand all processes that contribute to these effects. Forest harvesting best management practices (BMPs) focus almost exclusively on overland sediment sources, whereas in‐and‐near stream sources go unaddressed although they can contribute substantially to sediment yield. Thus, we propose a new framework to classify forest harvesting effects on stream sediment yield according to their direct and indirect processes. Direct effects are those caused by erosion and sediment delivery to surface water from overland sources (e.g., forest roads). Indirect effects are those caused by a shift in hydrologic processes due to tree removal that accounts for increases in subsurface and surface flows to the stream such that alterations in water quality are not predicated upon overland sediment delivery to the stream, but rather in‐stream processes. Although the direct/indirect distinction is often implicit in forest hydrology studies, we have formalized it as a conceptual model to help identify primary drivers of sediment yield after forest harvesting in different landscapes. Based on a literature review, we identify drivers of these effects in five regions of the United States, discuss current forest management BMPs, and identify research needs.