Biodegradation of carbofuran in soils within Nzoia River Basin,Kenya

Carbofuran (2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate) has been used within the Nzoia River Basin (NRB), especially in Bunyala Rice Irrigation Schemes, in Kenya for the control of pests. In this study, the capacity of native bacteria to degrade carbofuran in soils from NRB was investigated. A gram positive, rod-shaped bacteria capable of degrading carbofuran was isolated through liquid cultures with carbofuran as the only carbon and nitrogen source. The isolate degraded 98% of 100-μg mL^?1 carbofuran within 10 days with the formation of carbofuran phenol as the only detectable metabolite. The degradation of carbofuran was followed by measuring its residues in liquid cultures using high performance liquid chromatography (HPLC). Physical and morphological characteristics as well as molecular characterization confirmed the bacterial isolate to be a member of Bacillus species. The results indicate that this strain of Bacillus sp. could be considered as Bacillus cereus or Bacillus thuringiensis with a bootstrap value of 100% similar to the 16S rRNA gene sequences. The biodegradation capability of the native strains in this study indicates that they have great potential for application in bioremediation of carbofuran-contaminated soil sites.     

Suppressed recovery of plant community composition and biodiversity on dredged fill of a hurricane-induced inlet through a barrier island

In September 2003, Hurricane Isabel created an inlet over 500 m wide and 10 m deep that connected the Atlantic Ocean and Pamlico Sound. This breach was subsequently filled with sediments dredged from the adjacent sound. The purpose of this study was to determine if the barrier island terrestrial plant communities were naturally re-establishing through primary succession. In 2006–2008, we compared plant communities, soil carbon and nitrogen, and Aeolian transport of sediments in undisturbed back-dunes, undisturbed shrub thickets, putative back-dunes, and putative shrub thickets. We found that species richness and evenness were low on the filled area relative to adjacent plant communities that had persisted through the storm. Plants on the filled area were almost entirely limited to a band of primarily Spartina patens found at the margin of the sound and there were no signs of establishing the typical zonation of back dune grasses, shrubs, and salt marsh. Evaluation of soil quality suggests that nutrients and organic material are not limiting recovery. Aeolian transport, however, was demonstrably higher across the filled area, where no dense stands of taller plants buffered the airflow. Plant re-establishment is suppressed by wind erosion inhibiting deposition of seeds. Recovery of the site will likely depend on the rhizomatous spread of S. patens from the sound shore. S. patens can then potentially facilitate the colonization of other species by buffering the wind and trapping seeds of other plants. Ironically, this slow recovery may benefit federally threatened bird species that require sparse vegetation for nesting success.     

INSTITUTIONAL BARRIERS TO WATER MARKETING IN THE WEST1

ABSTRACT: Water marketing has been proposed as one means of reallocating water supplies in the western United States. While markets for western water currently exist and may be expected to expand, the institutional constraints within which the markets must operate will limit the ultimate size and efficiency of those markets. Lack of articulation of public interests in the water resource itself leads to incomplete definition of the private rights to use the water, and it is those private rights which are sold or leased in the market. The increase in size and efficiency of any market in water rights will be dependent on the willingness of legislatures to specifically define the nature and extent of public interests in water supplies, and detailed definition is unlikely.     

INTERSTATE AND INTERNATIONAL COMPETITION FOR WATER RESOURCES1

ABSTRACT: Federal-state relations over the management and regulation of ground water resources have undergone a number of changes in recent years. There are a variety of reasons that the role of the federal government in ground water management may increase in the future. One important reason federal policymakers may become involved concerns the extent of competition for ground water resources that cross state and national borders. This article summarizes reasons that interstate and international competition for ground water resources may provide the impetus for federal intervention in ground water management and presents the results of a survey conducted to determine the extent of that competition.     

Risk-based standards: integrating top–down and bottom–up approaches

In response to rapidly changing threats posed to increasingly complex socio-technical systems, many in the government and private sector have called for protection through risk-based standards. However, given the nature of these dynamic and uncertain threats, traditional risk assessment techniques may not be sufficient. Instead, there is a critical need for an integrated approach in which decision analytic techniques are used to assess evidence-based data with the values and preferences of decision makers. We point to three examples in the fields of nuclear power regulation, nanotechnology, and cybersecurity, where risk-based approaches (bottom–up) have been combined with decision analysis (top–down) to guide decision makers toward risk management policies that manifest both the best available evidence and the plurality of values within a society.     

Cell-free DNA fetal fraction in twin gestations in single-nucleotide polymorphism-based noninvasive prenatal screening

Objectives

The performance of noninvasive prenatal screening (NIPS) for fetal aneuploidy in twin pregnancies is dependent on the amount of placentally derived cell-free DNA, the “fetal fraction (FF),” present in maternal plasma. We report FF values in monozygotic (MZ) and dizygotic (DZ) pregnancies.

Methods

We reviewed FF in pregnancies at 10 to 20 completed weeks gestational age based on single-nucleotide polymorphism (SNP)-based NIPS where zygosity was routinely established in twin pregnancies. The cohort included 121 446 (96.3%) singleton, 1454 (1.2%) MZ, and 3161 (2.5%) DZ pregnancies. For DZ twins, individual FFs were measured.

Results

Combined FF for DZ and MZ fetuses were 35% and 26% greater than singletons, respectively. The individual FF contributions from each fetus in DZ twins were, on average, 32% less than singletons. FF in DZ twin pairs were moderately correlated (Pearson correlation coefficient.66). When a threshold of 2.8% FF was applied to define uninterpretable results, 1.7% (2102/121 446) of singletons, 0.8% (11/1454) of MZ pairs, and 5.6% (178/3161) of DZ pairs were uninterpretable.

Conclusion

For optimal aneuploidy NIPS in twin pregnancies, zygosity should be established and in DZ twins FF for both fetuses should be determined to identify those cases where results can be reliably interpreted.     

Analyzing interdependent impacts of resource sustainability

Quantifying the impact of the shortage of a scarce resource requires a systemic account of the interdependent nature of several industry and infrastructure sectors that rely either directly or indirectly on that resource. An ability to quickly and easily quantify such an impact provides policymakers with a useful measure of the efficacy of discovering, designing, or developing a sustainable alternative. Discussed in this paper is a methodological approach for measuring the broader interdependent impacts of a resource shortage. The dynamic inoperability input–output model (DIIM) is used to illustrate both the economic effects of resource shortages over a period of time and the time-dependent recovery of industry sectors. Extensions to the DIIM are introduced to produce an accessible tool for policymakers and industry decision makers. Case studies using publicly available data illustrate the usefulness of the model for describing local oil production shortages and global rare earth metals supply shortages, highlighting the industries that will need to adapt to changes in resource availability, as well as those industries that will remain relatively unaffected. Above all, the model presented in this paper is an effective means of communicating the impact and importance of resource shortages to assist in the design and development of a sustainable future.     

Implications of maternal serum alpha-fetoprotein elevation caused by transabdominal and transcervical CVS

Of 2882 women allocated to either transabdominal CVS (TA) or transcervical CVS (TC) at two large obstetric centres in Denmark, 2707 had blood samples drawn before and 30 min after CVS for maternal serum-alpha-fetoprotein (MSAFP) measurement. 2535 of these women had cytogenetically normal pregnancies and 2091 of them went on to have samples drawn at the 18–20 week follow-up. Post-procedure MSAFP values were correlated to the biopsy method used, with mean MSAFP values significantly higher after TA than TC, 33 and 15 kU/l, respectively (P<0·001). Following TA procedures, 18 per cent of cases had feto-maternal transfusion higher than 0·1 ml; this occurred in only 5 per cent of TC cases. MSAFP levels were associated with spontaneous fetal loss in the TA group but not in the TC group. TC, however, was followed by more losses than TA. The post-CVS MSAFP value was positively correlated with the amount of villi aspirated. The difference in post-procedure elevation in MSAFP 30 min later (average 18 kU/l higher for TA than for TC) was not reflected in raised levels at the 18–20 week follow-up. Study medians at mid-trimester did not differ from reference group medians established from a group of singleton pregnancies with sonographically determined gestational age who did not experience invasive procedures and delivered normal infants. Our findings suggest that CVS does not compromise mid-trimester MSAFP for screening for neural tube defects (NTDs). Extremely high mid-trimester MSAFP values in the TC group could predict imminent loss.