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21.
Fragile X syndrome is the most common cause of familial mental retardation. The most common mutation is expansion of a triplet (CGG)n repeat in the 5′ untranslated region of the FMR1 gene on Xq27.3. The expansion is refractory to PCR due to preferential amplification of the smaller allele in heterozygous cells and the high GC content of the repeat and surrounding sequences. Direct detection of the normal parental alleles in preimplantation embryos has been used for preimplantation genetic diagnosis (PGD) of this disorder. However, this approach is only suitable for approximately 63% of couples due to the heterozygosity of the repeat in the normal population. As an alternative we investigated the use of polymorphic markers flanking the mutation to track the normal and premutation carrying maternal chromosomes in preimplantation embryos. Using a panel of 11 polymorphisms, six (CA)n repeats and five single nucleotide polymorphisms, diagnosis was developed for 90% of referred couples. Multiplex amplification of informative markers was tested in 300 single buccal cells from interested couples with efficiency and allele drop out (ADO) rates ranging from 69% to 96% and 6% to 18%, respectively. Use of this approach is accurate and applicable to a larger number of patients at risk of transmitting fragile X to their offspring. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
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A. V. Krylov 《Russian Journal of Ecology》2004,35(5):316-323
The abundance and trophic structure of zooplankton along the longitudinal profile of two typical rivers in the Yaroslavl sector of the Volga region are determined by anthropogenic and zoogenic factors. The distribution of zooplankton under the influence of these factors is described by the concept of patch dynamics. The abundance of zooplankton reaches the highest values in the ameliorated upper reaches of rivers and in beaver ponds. 相似文献
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Quick fix. Putting out firs. Do it over. Fix it again. Bottlenecks. Come back to it later. Out of stock. Back order. Not what we ordered. Is that the best you can do? It's the contractor's fault. Not within specs. Defective. Nobody's perfect. Lost in the system. Over budget. Delayed. Late penalities. Fines. 相似文献
27.
A. L. Heathwaite 《Chemistry and Ecology》1993,8(3):217-231
Although most research has focussed on inorganic nutrient forms of nitrate (NO-3) and phosphorus (PO34) in runoff and receiving waters, nitrogen loss from agricultural land can also occur in organic and ammonium-nitrogen form; phosphorus losses, although often dominated by particulate transport, may occur in soluble organic and inorganic form. Furthermore, fluxes between different species may take place during transport from the land to the stream and as a result of in-stream, in-river or in-lake transformations. Knowledge of the spatial and temporal variation in all nitrogen species and phosphorus fractions in a drainage basin is therefore essential if the wider environmental significance of elevated nutrient concentrations in natural waters are to be assessed. This paper reviews recent work on N and P losses from agricultural land and presents some results from two intensive agricultural catchments: Slapton, Devon and the river Windrush catchment in the Cotswolds. 相似文献
28.
J. Malczewski R. Moreno-Sanchez L.A. Bojorquez-Tapia E. Ongay-Delhumeau 《Journal of Environmental Planning and Management》1997,40(3):349-374
This paper is concerned with developing a model for group decision making under multiple criteria. The multiple criteria group decision making (MCGDM)problem involves a set of feasible land use patterns that are evaluated on the basis of multiple, conflicting and noncommensurate criteria by a group of individuals. The model integrates the Analytic Hierarchy Process (AHP) and an integer mathematical programming method. The former provides a tool for structuring the decision problem and determining land suitability for different socio-economicactivities (the uses of land), the latter is used to identify the land use pattern that maximizes consensus among interest groups. The model is used to analyze environmental conflict over land resource allocation in the Cape Region of Mexico. 相似文献
29.
María L. Pignata Martha S. Cañas Hebe A. Carreras Liliana Orellana 《Environmental management》1997,21(5):793-801
Ligustrum lucidum Ait. f. tricolor (Rehd.) Rehd. in relation to atmospheric pollutants in Córdoba city, Argentina. The study area receives
regional pollutants and was categorized taking into account traffic level, industrial density, type of industry, location
of the sample point in relation to the street corner, treeless condition, and topographic level. Dried weight/fresh weight
ratio (DW/FW) and specific leaf area (SLA) were calculated, and concentrations of chlorophylls, carotenoids, total sulfur,
soluble proteins, malondialdehyde (MDA), and hydroperoxy conjugated dienes (HPCD) were determined in leaf samples. Sulfur
content correlates positively with traffic density and SLA correlates negatively with some combinations of the categorical
variables; MDA correlates positively with topographic level and total protein concentration correlates negatively with treeless
condition. On the basis of our results, traffic, location of trees, type of industry, situation of a tree with respect to
others, and topographic level are the environmental variables to bear in mind when selecting analogous sampling points in
a passive monitoring program. An approximation to predict tree injury may be obtained by measuring DW/FW ratio, proteins,
pigments, HPCD, and MDA as they are responsible for the major variability of data. 相似文献
30.
This paper summarizes studies of carbon mitigation potential (MP) and costs of forestry options in seven developing countries with a focus on the role of agroforestry. A common methodological approach known as comprehensive mitigation assessment process (COMAP) was used in each study to estimate the potential and costs between 2000 and 2030. The approach requires the projection of baseline and mitigation land-use scenarios derived from the demand for forest products and forestland for other uses such as agriculture and pasture. By using data on estimated carbon sequestration, emission avoidance, costs and benefits, the model enables one to estimate cost effectiveness indicators based on monetary benefit per t C, as well as estimates of total mitigation costs and potential when the activities are implemented at equilibrium level. The results show that about half the MP of 6.9 Gt C (an average of 223 Mt C per year) between 2000 and 2030 in the seven countries could be achieved at a negative cost, and the other half at costs not exceeding $100 per t C. Negative cost indicates that non-carbon revenue is sufficient to offset direct costs of about half of the options. The agroforestry options analyzed bear a significant proportion of the potential at medium to low cost per t C when compared to other options. The role of agroforestry in these countries varied between 6% and 21% of the MP, though the options are much more cost effective than most due to the low wage or opportunity cost of rural labor. Agroforestry options are attractive due to the large number of people and potential area currently engaged in agriculture, but they pose unique challenges for carbon and cost accounting due to the dispersed nature of agricultural activities in the tropics, as well as specific difficulties arising from requirements for monitoring, verification, leakage assessment and the establishment of credible baselines. 相似文献