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161.
dos Santos Debora Regina Lopes Silva-Sales Marcelle Fumian Tulio Machado Maranhão Adriana Gonçalves Malta Fábio Correia Ferreira Fernando César Pimenta Marcia Maria Miagostovich Marize Pereira 《Food and environmental virology》2023,15(1):21-31
Food and Environmental Virology - This study assessed the sources of contamination of water matrices in a rural area using detection of a host-specific virus (human adenovirus [HAdV], porcine... 相似文献
162.
Marina Medvedova Adriana Kolesarova Marcela Capcarova Roman Labuda Alexander V. Sirotkin Jaroslav Kovacik 《Journal of environmental science and health. Part. B》2013,48(3):213-219
The aim of this in vitro study was to examine the secretion activity, markers of proliferation and apoptosis in porcine ovarian granulosa cells (GCs) after deoxynivalenol (DON) addition. Ovarian granulosa cells were incubated with DON for 24h: 10, 100 and 1000 ng/mL, while the control group received no DON. The secretion of insulin-like growth factor I (IGF–I) and progesterone was determined by radioimmunoassay (RIA) and expression of cyclin B1, PCNA and caspase-3 by immunocytochemistry. IGF–I release by GCs was inhibited by DON, while progesterone release and the expression of cyclin B1 was stimulated by DON (at 1000 ng/mL but not at 10 and 100 ng/mL). PCNA expression was stimulated by DON (at 100 and 1000 ng/mL but not at 10 ng/mL). Caspase-3 expression was not influenced by DON treatment (at all doses). In conclusion, our results indicate, (1) a direct effect of DON on secretion of growth factor IGF-I and steroid hormone progesterone, (2) expression of markers of proliferation (cyclin B1 and PCNA) but not on the (3) expression of marker of apoptosis (caspase-3) in porcine ovarian granulosa cells. This in vitro study suggests the dose-dependent association of DON on porcine ovarian functions. 相似文献