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51.
Preimplantation genetic diagnosis (PGD) is an alternative to prenatal diagnosis for couples at risk of transmitting genetic disorders to their offspring. We present a fluorescence in situ hybridization (FISH) analysis of embryos obtained after seven PGD cycles in six couples with Robertsonian translocations and male factor infertility: 4 der(13;14), 1 der(14;21) and 1 der(15;21). Of 74 metaphase II (MII) injected oocytes, 61 (82.4%) fertilized normally and cleaved. Of these, 37/61 (60.7%) embryos were of high morphological quality with ≥6 blastomeres. After biopsy of 44 embryos at day 3 of development, seven degenerated, seven arrested in development and 30/44 (68.2%) evolved, of which 25/30 (83.3%) reached the morula/blastocyst stage. Analysis of biopsied blastomeres showed 23/44 (52.3%) of normal/balanced embryos, of which 15 (11 at the morula/blastocyst stage) were transferred in six cycles. One term pregnancy was achieved, which ended by cesarean section at 37 weeks of gestation, giving birth to two healthy newborn. Analysis of 49 embryos (excluding 12 inconclusive cases) showed a predominance of alternate segregation (38/49, 77.6%) over adjacent segregation (7/49, 14.3%), with one (2%) being a polyploid mosaic and three (6.1%) chaotic. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
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54.
Alberto Martinetti Maria Mikela Chatzimichailidou Luisa Maida Leo van Dongen 《International journal of occupational safety and ergonomics》2019,25(1):66-75
Occupational health and safety (OHS) represents an important field of exploration for the research community: in spite of the growth of technological innovations, the increasing complexity of systems involves critical issues in terms of degradation of the safety levels. In such a situation, new safety management approaches are now mandatory in order to face the safety implications of the current technological evolutions. Along these lines, performing risk-based analysis alone seems not to be enough anymore. The evaluation of robustness, antifragility and resilience of a socio-technical system is now indispensable in order to face unforeseen events. This article will briefly introduce the topics of Safety I and Safety II, resilience engineering and antifragility engineering, explaining correlations, overlapping aspects and synergies. Secondly, the article will discuss the applications of those paradigms to a real accident, highlighting how they can challenge, stimulate and inspire research for improving OHS conditions. 相似文献
55.
Carlos Toledo-Hernández Alberto M. Sabat Anabella Zuluaga-Montero 《Marine Biology》2007,152(3):527-535
Gorgonia ventalina’s density, size structure and lesion prevalence was measured at six sites in Puerto Rico that exhibited variation in horizontal
water transparency, sedimentation rates, suspended particle matter, scleractinian and macroalgal cover. G. ventalina density varied significantly among sites (between 0.84 and 0.007 colonies/m2), and was positively correlated with water transparency. Size structure did not vary much among sites, and reflects high
mortality among the smaller size classes and high survivorship in large colonies. Prevalence of active fungi-induced lesions
(type I) did not vary significantly among sites and was density-independent. However, prevalence of old lesions of unknown
origin (type II) did vary among reefs and was negatively correlated with water transparency. Prevalence of types I or II lesions
was independent of colony size. Our results suggest that (1) turbidity and sedimentation are important abiotic factors controlling
the abundance of sea fans, (2) variation in settlement success and early survivorship of recruits has more impact on the sea
fan populations than variation in the survivorship of large colonies and (3) prevalence of aspergillosis (type I) at the studied
sites is similar to that reported for other Caribbean reefs and supports the epizootic nature of the disease and (4) lesions
with exposed skeleton are more likely to be colonized by fouling organisms at impacted reefs. The combined effects of anthropogenic
impacts and aspergillosis may cause local extinctions of sea fans, as is becoming evident in many reefs in Puerto Rico. 相似文献
56.
We investigated the physiological plasticity and environmental tolerance of two phylogenetically closely related, ecologically
similar and co-occurring species of supralittoral amphipods differing drastically in the size of their geographical ranges.
A series of physiological traits were characterised for the Corsican-endemic Talorchestia ugolinii Bella-Santini and Ruffo and the widespread Talitrus saltator Montagu. The effect of body mass, temperature and salinity on heart rate (used as proxy for metabolic activity and stress),
the effect of temperature on oxygen consumption and the tolerance to salinity exposure were investigated in both species,
together with the characterisation of haemolymph osmoregulation in T. ugolinii. Our results showed that there is a clear difference in the resting metabolic rates and physiological capacity, as well as
environmental tolerance, between T. saltator and T. ugolinii, with T. saltator overall showing a broader physiological niche. Although T. ugolinii showed a relatively good ability to regulate its haemolymph osmotic concentration (similar to that previously described for
T. saltator), it demonstrated a lower tolerance to exposure to hypo-osmotic stress. In addition, a consistent picture emerged between
the ability to control the cardiac function and the capacity to actively respond to osmotic stress. The physiological findings
are discussed in relation to the known ecology and geographical distribution of T. ugolinii. 相似文献
57.
Abdallah Dahbi Alain Lenoir Alberto Tinaut Timo Taghizadeh Wittko Francke Abraham Hefetz 《Chemoecology》1996,7(4):163-171
Summary A comparative morphological and chemical study of six endemic species ofCataglyphis from the Iberian Peninsula:C. ibericus, C. rosenhaueri, C. hispanicus, C. humeya, C. velox andC. floricola and the Moroccan speciesC. bombycinus is described. The morphological study relied primarily on genitalia characteristics, whereas the chemical study concentrated on the postpharyngeal gland constituents. Cladograms based on the morphological and chemical data were performed using Ward's method. The dendrogram based on morphological features revealed that the IberianCataglyphis can be classified into three species groupsalbicans, altisquamis andemmae. The same pattern occurred when the dimethylalkanes constituents of the postpharyngeal gland were utilised as character states, with a slight displacement of species within thealtisquamis group. However, when the complete hydrocarbon blend was utilised major discrepancies in the dendrograms occurred.Cataglyphis velox proved to be very similar toC. bombycinus, whereasC. floricola clustered with the other two species of thealtisquamis group. Based on the geographical distribution and paleontological data (Tinaut 1993) it is assumed thatC. floricola recently invaded the Iberian Peninsula. Based on the chemical findings we postulate that chemical character displacement occurred inC. floricola as a result of its sympatry withC. velox after the former colonized the Iberian Peninsula. We further discuss the possible reason for the different dendrograms obtained when only the dimethylalkanes are considered and its implication for the communicative role of the postpharyngeal gland secretion in these ants. 相似文献
58.
Open-focused microwave-assisted extraction and ICP-OES determination of As, Cd, Cr, Cu, Fe, Mn, Ni, Pb and Zn from surface sediments of the estuary of Bilbao (Basque Country, North of Spain) was carried out. All the samples were collected at three different tributaries of the estuary (Asua, Galindo and Nerbioi-Ibaizabal) every two months during 1999. The digestion procedure was proposed from the conclusions of a fractionated factorial design, and the precision and accuracy of the method was verified using a certified reference sediment (RTC008-050). The results of the analysis were statistically treated by means of principal component analysis and correlation analysis. The principal component analysis of sediment data (32 samples × 9 metals) indicated different patterns of contamination regarding the tributary and sampling station. The two main patterns observed were a steady increment of the metal concentration along all the campaigns in the samples collected in the Galindo River and a seasonal variation in the Nerbioi-Ibaizabal River, with higher metallic content during summertime and lower content during wintertime. 相似文献
59.
Pereira FA de Assunção JV Saldiva PH Pereira LA Mirra AP Braga AL 《Journal of the Air & Waste Management Association (1995)》2005,55(1):83-87
To evaluate the possible influence of air pollutants on the incidence of respiratory tract neoplasm we studied 12 S?o Paulo City districts where air pollution is observed. We collected data on pollutants from 1981-1990 and correlated it with the incidence of larynx and lung neoplasms in 1997. Ozone was the pollutant that best correlated with the incidence of neoplasm. The correlation coefficients were 0.7234 (p=0.277) and 0.9929 (p=0.007) for lung and larynx cancer, respectively. There may be a relationship between air pollution and the incidence of respiratory tract neoplasm. This study, however, does not allow us to conclude that there is a causal relationship. 相似文献
60.
Orrù R Cincotti A Concas A Cao G 《Environmental science and pollution research international》2003,10(6):385-389
The possibility of exploiting self-propagating reactions for environmental protection is discussed in this paper. In particular, results obtained at the laboratory scale and related to the fixation and consolidation of high level radioactive wastes, the recycling of silicon sludge and aluminum dross produced by semiconductor industries and aluminum foundries, the treating and recycling of a highly toxic solid waste from electrolytic zinc plants, and the degradation of chlorinated aromatics, are examined with particular emphasis on the latter case. Specifically, the self-propagating destruction of hexachlorobenzene and 2-(2-4-dichlorophenoxy)-propanoic acid with calcium hydride as reductive substrate is demonstrated. In fact, the heat liberated by the reactions involved is large enough to guarantee the self-sustaining character of the process within a wide range of reactants compositions. Moreover, no residual chlorinated organic compounds were found in the final solid product. Some reactor engineering aspects, as well as other significant future scientific and technological issues, are also addressed in view of large-scale applicability of processes based on self-propagating reactions. To date, the batch reactor technology seems to be more easily applicable, although the use of continuous reactors is not excluded in the next future. 相似文献