Establishment patterns in a secondary tree line ecotone

On semi-open pre-alpine fen pastures Alder encroachment creates a dynamic mosaic of grassland and woodland, which is rich in ecotones from fen to Carr. The structural diversity in colonisation patterns of Alder on fens suggests a dependency on multiple environmental drivers. Unidirectional progressive ecotone development provides an opportunity to address a current deficit in understanding successional patterns, i.e. process-pattern relationships in a multiple factor regime.We developed an individual-based model of Alder establishment on fen grassland to investigate the dependency of encroachment patterns upon seed production, dispersal distances and safe site availability. The purpose of the model is to provide a causal understanding of establishment patterns of Alder. In the model, all life processes of Alder individuals were parameterised with field data. This allowed us to strictly perform bottom-up simulations and successfully check plausibility by comparing simulated establishment patterns of cohorts with observed ecotone structures.Simulation results show that establishment patterns strongly depend on environmental drivers. Spatial progression of Alder encroachment and width of ecotones, respectively, mainly depend on wind speed during seed dispersal. Dense establishment of Alder leading to community change from fen grassland to Carr, requires windows of opportunity, which are defined by the rare coincidence of widespread dispersal, high seed production and favorable establishment conditions. Life-history traits of Alder (mast year cycle, high seed weight, weak establishment in fen) spatially and temporarily constrain the encroachment process. The structural diversity of long-term encroachment patterns is explained by the event-driven character of encroachment.Modelling individual establishment pathways of seedlings starting from germination revealed an endogenous stochasticity in establishment patterns emerging from low seed densities in the tail of the dispersal function. We conclude an inherent stochastic structure of dispersal-limited tree line ecotones, which limits reconstruction of processes from patterns.In order to describe long-term successional patterns of Alder encroachment at landscape scale, we propose the combination of two concepts: deterministic “patch-movement” of Alder woodland driven by continuous ecotone migration together with rare and stochastic “infiltration” of single Alder trees into open fen grasslands. Conservation management can control predictable “patch-movement” by cutting off maturing saplings around existing Alder woods. But the preservation of the actual large proportion of open grassland in fen pastures from infiltrating Alder seedlings and from the subsequent shift of the pasture to a densely wooded state would require mowing additionally to extensive grazing.     

Influence of aerobic and anaerobic conditions on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in Luria-Bertani broth, farm-yard manure and slurry

The influence of aerobic and anaerobic conditions on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium was investigated in microcosms with broth, cattle manure or slurry. These substrates were inoculated with a green fluorescent protein transformed strain of the enteropathogens at 10(7) cells g(-1) dry weight. Survival data was fitted to the Weibull model. The survival curves in aerobic conditions generally showed a concave curvature, while the curvature was convex in anaerobic conditions. The estimated survival times showed that E. coli O157:H7 survived significantly longer under anaerobic than under aerobic conditions. Survival ranged from approximately. 2 weeks for aerobic manure and slurry to more than six months for anaerobic manure at 16 °C. On average, in 56.3% of the samplings, the number of recovered E. coli O157:H7 cells by anaerobic incubation of Petri plates was significantly (p < 0.05) higher in comparison with aerobic incubation. Survival of Salmonella serovar Typhimurium was not different between aerobic and anaerobic storage of LB broth or manure as well as between aerobic and anaerobic incubation of Petri dishes. The importance of changes in microbial community and chemical composition of manure and slurry was distinguished for the survival of E. coli O157:H7 in different oxygen conditions.     

The Analysis of Sustainability Indicators as Socially Constructed Policy Instruments: benefits and challenges of 'interactive research'

Sustainability indicator sets are increasingly being discussed on the policy level as fruitful contributions to the improvement of political decision- making and to the implementation of programs oriented towards the achievement of strategic goals of sustainable development. The vast number of different indicator type tools, their varying contexts of use and their differing objectives indicate that there is no simple answer to what sustainable indicator type tools should look like or could be used for. Instead, more than the final products (e.g. a specific indicators set), the analyses of the discourse on this topic reveal a lot of information. Thus, an innovative research approach is recommended focusing on understanding the production of social meaning and processes of social interaction within political-administrative systems. Firstly, there is a need to identify the development, purpose and use of sustainability indicator sets, which depend on the different interests of policy actors, their relationships and existing governance structures. Secondly, one should identify any reasons for the ineffective use of indicator sets where the goals of sustainability are concerned. The approach of 'interactive research' understood as a research process, in which 'researchers' and 'practitioners' develop knowledge for solving problems in a communicative, reflexive and collaborative way, facilitates this challenging research task. This paper critically examines the approach of interactive research and sheds some light on benefits as well as challenges of it via extracting the lessons learnt in an EU-funded project called 'Promoting Action for Sustainability through Indicators at the Local Level in Europe' (PASTILLE), which applied an interactive research approach.     

Interfaces in Cross-Linked and Grafted Bacterial Cellulose/Poly(Lactic Acid) Resin Composites

This article presents approaches to maximize the mechanical performance of bacterial cellulose/poly(lactic acid) composites through chemical modification of the interface. This is achieved by both cross-linking the layered bacterial cellulose structure and by grafting maleic anhydride to the matrix material. Unmodified and glyoxalized bacterial cellulose (BC) networks have been embedded in poly(lactic acid) (PLA) resin and then in maleated resin using a compression molding method. The effect of these chemical modifications on the physical properties of these composites is reported. The tensile properties of the composites showed that Young??s moduli can be increased significantly when both BC networks and PLA were chemically modified. Interface consolidation between layers in BC networks has been achieved by glyoxalization. The effect of these modifications on both stress-transfer between the fibers and between the matrix and the fibers was quantified using Raman spectroscopy. Two competitive deformation mechanisms are identified; namely the mobility between BC layers, and between BC and PLA. The coupling strength of these interfaces could play a key role for optimization of these composites?? mechanical properties.     

Urban stream syndrome in a small,lightly developed watershed: a statistical analysis of water chemistry parameters,land use patterns,and natural sources

The relationships among land use patterns, geology, soil, and major solute concentrations in stream water for eight tributaries of the Kayaderosseras Creek watershed in Saratoga County, NY, were investigated using Pearson correlation coefficients and multivariate regression analysis. Sub-watersheds corresponding to each sampling site were delineated, and land use patterns were determined for each of the eight sub-watersheds using GIS. Four land use categories (urban development, agriculture, forests, and wetlands) constituted more than 99 % of the land in the sub-watersheds. Eleven water chemistry parameters were highly and positively correlated with each other and urban development. Multivariate regression models indicated urban development was the most powerful predictor for the same eleven parameters (conductivity, TN, TP, NO $_{3}^-$ , Cl^?, HCO $_{3}^-$ , SO $_{4}^{2-}$ , Na⁺, K⁺, Ca²⁺, and Mg²⁺). Adjusted R ² values, ranging from 19 to 91 %, indicated that these models explained an average of 64 % of the variance in these 11 parameters across the samples and 70 % when Mg²⁺ was omitted. The more common R ², ranging from 29 to 92 %, averaged 68 % for these 11 parameters and 72 % when Mg²⁺ was omitted. Water quality improved most with forest coverage in stream watersheds. The strong associations between water quality variables and urban development indicated an urban source for these 11 water quality parameters at all eight sampling sites was likely, suggesting that urban stream syndrome can be detected even on a relatively small scale in a lightly developed area. Possible urban sources of Ca²⁺ and HCO $_{3}^-$ are suggested.     

Subterranean freshwater gastropod biodiversity and conservation in the United States and Mexico

Many taxonomic groups successfully exploit groundwater environments and have adapted to a subterranean (stygobiotic) existence. Among these groups are freshwater gastropods (stygosnails), which represent a widespread and taxonomically diverse component of groundwater ecosystems in North America. However, owing to sampling difficulty and lack of targeted study, stygosnails remain among the most understudied of all subterranean groups. We conducted a literature review to assess the biodiversity and geographic associations of stygosnails, along with the threats, management activities, and policy considerations related to the groundwater systems they inhabit. We identified 39 stygosnail species known to occur in a range of groundwater habitats from karst regions in the United States and Mexico. Most stygosnails exhibit extreme narrow-range endemism, resulting in a high risk of extinction from a single catastrophic event. We found that anthropogenically driven changes to surface environments have led to changes in local hydrology and degradation of groundwater systems inhabited by stygosnails such as increased sedimentation, introduction of invasive species, groundwater extraction, or physical collapse of water-bearing passages. Consequently, 32 of the 39 described stygosnail species in the United States and Mexico have been assessed as imperiled under NatureServe criteria, and 10 species have been assessed as threatened under International Union for Conservation of Nature criteria. Compared with surface species of freshwater snails, stygosnail conservation is uniquely hindered by difficulties associated with accessing subterranean habitats for monitoring and management. Furthermore, only three species were found to have federal protection in either the United States or Mexico, and current laws regulating wildlife and water pollution at the state and federal level may be inadequate for protecting stygosnail habitats. As groundwater systems continue to be manipulated and relied on by humans, groundwater-restricted fauna such as stygosnails should be studied so unique biodiversity can be protected.