Comparative study of the sensitivity to cadmium of two populations of Gambusia affinis from two different sites

This study aims to demonstrate the influence of animals' origin on their sensitivity toward heavy metals. For this purpose, we compared LC(50) of cadmium in two populations of Gambusia affinis captured in two geographically isolated environments in the east of Tunisia; Oued El Gsil in the city of Monastir (S2) and Oued Chenini in the region of Gabes (S1). Although physicochemical parameters of the water (pH, dissolved oxygen and salinity) are similar in the two studied sites, cadmium concentrations in water, sediments and fish tissues from S1 are significantly higher (P < 0.01) than those from S2, 48-h and 96-h LC(50) of the (S1) population are significantly higher than those from S2. In the same way, the offspring of the polluted site (S1) population exhibit 48-h and 96-h LC(50) values much higher than those of the reference site (S2) population. These results show that the population of the Gabes region is more resistant to cadmium than that of the Monastir region and that this resistance could have a genetic basis. These results indicate the influence of the origin of animals that has to be taken into account not only in laboratory toxicity tests, but also in field ecotoxicological studies.     

Spatiotemporal changes of landscape pattern in response to deforestation in Northeastern Turkey: a case study in Rize

Recognition and understanding of landscape dynamics as a historical legacy of disturbances are necessary for sustainable management of forest ecosystems. This study analyzes spatial and temporal changes in land use and forest cover patterns in a typical mountain forest area in Rize Forest Enterprise of the Northeastern part of Turkey. The area is investigated by evaluated the temporal changes of spatial structure of forest conditions through spatial analysis of forest cover type maps from 1984 and 2007 using GIS and FRAGSTATS. The quantative evidences presented here showed that there were drastic changes in the temporal and spatial dynamics of land use/forest cover. As an overall change between 1984 and 2007, there was a net decrease of 2.30% in total forested areas. On one hand, productive forest areas decreased 12,506 ha, on the other hand, degraded forest areas increased 14,805 ha. In examining the changes of crown closure and development stages of forest ecosystem during the study period, the forest stand area with medium crown closures increased. Regenerated area increased while the other development stages were left to grow to mature development stages in the period. These results regarding to crown closure and development stage showed that forest quality has increased but total forest areas decreased. This is partially due to out-migration of rural population in Rize and Cayeli towns. In terms of spatial configuration, analysis of the metrics revealed that landscape structure in Study area had changed substantially over the 23-year study period, resulting in fragmentation of the landscape as indicated by the large patch numbers and the smaller mean patch sizes due to heavy timber subtraction, illegal cutting, and uncontrolled stand treatments.     

Assessment of trace metal concentrations in muscle tissue of certain commercially available fish species from Kayseri,Turkey

Regular consumption of fish has been widely recommended by health authorities. However, it is known that some species accumulate high levels of contaminants including heavy metals (e.g., Hg, Cd, Pb, and As). In this study, Cu, Pb, Co, Ni, Cr, Mn, Cd, and Fe were determined in the muscle tissue of 11 commercially available fish species (Sparus auratus, Dicentrarchus labrax, Mullus barbatus, Belone belone, Psetta maxima, Epinephelus aeneus, Salmothymus, Soleidae, Pomatomus saltatrix, Engraulis encrasicolus, and Sarda sarda) supplied from retailers in Kayseri, Turkey. Determinations were carried out by flame atomic absorption spectrometry after the wet digestion method. The average metal concentrations of the 11 species were determined in the range of 0.54–1.79, 0.82–1.40, 2.38–4.54, 1.23–3.67, 5.01–5.97, 0.77–3.59, 0.48–1.06, and 5.05–122.8 μg/g wet weight for Cu, Pb, Co, Ni, Cr, Mn, Cd, and Fe, respectively. The permissible tolerable daily intake (PTDI) and calculated daily intake (CDI) values were compared, and the calculated daily intake values of the samples were found to be below the established values. Correlations between the metal contents in samples were investigated by performing correlation tests with SPSS 13.0 for windows.     

Diversity of bacterial isolates during full scale rotary drum composting

Bacterial diversity of full scale rotary drum composter from biodegradable organic waste samples were analyzed through two different approaches, i.e., Culture dependent and independent techniques. Culture-dependent enumerations for indigenous population of bacterial isolates mainly total heterotrophic bacteria (Bacillus species, Pseudomonas species and Enterobacter species), Fecal Coliforms, Fecal Streptococci, Escherichia coli, Salmonella species and Shigella species showed reduction during the composting period. On the other hand, Culture-independent method using PCR amplification of specific 16S rRNA sequences identified the presence of Acinetobacter species, Actinobacteria species, Bacillus species, Clostridium species, Hydrogenophaga species, Butyrivibrio species, Pedobacter species, Empedobactor species and Flavobacterium species by sequences clustering in the phylogenetic tree. Furthermore, correlating physico-chemical analysis of samples with bacterial diversity revealed the bacterial communities have undergone changes, possibly linked to the variations in temperature and availability of new metabolic substrates while decomposing organics at different stages of composting.     

Effects of golpar (<Emphasis Type="Italic">Heracleum persicum</Emphasis> Desf.) and probiotics in drinking water on performance,carcass characteristics,organ weights,blood plasma constituents,and immunity of broilers

Increasing levels of Heracleum persicum (golpar) in drinking water were studied in broilers. Two hundred and forty-one-day-old male chickens were allocated to one of six treatments: control, without added phytogenics nor probiotics in drinking water, and probiotics at recommended manufacturer’s level (P) or 1.0, 1.5, 2.0, and 2.5 ml/l of golpar extract solution (G1, G1.5, G2, and G2.5 treatments, respectively) in drinking water. As a result of this study, no linear or quadratic trends in the feed intake (FI) and feed conversion rate (FCR) due to golpar supplementation were found. Body weight gain, final body weight, and relative carcass weight showed a positive linear response with increasing levels of golpar supplementation. Neither golpar nor probiotics had effects on the percentages of edible parts of the carcass. Golpar supplementation levels caused a linear negative response of the albumin content in blood plasma, whereas both abdominal fat as percentage of carcass weight and uric acid levels in blood plasma linearly increased. The effects on Ig responses were only observed at 42 days of age and were similar in probiotics and the highest level of golpar supplementation. Based on our results, both probiotics and golpar supplementation could improve broiler performance and immune function.     

Municipal solid waste effective stress analysis

The mechanical behavior of municipal solid waste (MSW) has attracted the attention of many researchers in the field of geo-environmental engineering in recent years and several aspects of waste mechanical response under loading have been elucidated. However, the mechanical response of MSW materials under undrained conditions has not been described in detail to date. The knowledge of this aspect of the MSW mechanical response is very important in cases involving MSW with high water contents, seismic ground motion and in regions where landfills are built with poor operation conditions. This paper presents the results obtained from 26 large triaxial tests performed both in drained and undrained conditions. The results were analyzed taking into account the waste particles compressibility and the deformation anisotropy of the waste samples. The waste particles compressibility was used to modify the Terzaghi effective stress equation, using the Skempton (1961) proposition. It is shown that the use of the modified effective stress equation led to much more compatible shear strength values when comparing Consolidated-Drained (CD) and Consolidated-Undrained (CU), results, explaining the high shear strength values obtained in CU triaxial tests, even when the pore pressure is almost equal to the confining stress.     

Quantitative gap analysis for the West Coast of Florida based on experts’ opinion

The primary objective of this study was to identify gaps in coastal and marine data available through the Gulf Geospatial Assessment of Marine Ecosystems (GAME) project for the west coast of Florida. A survey was designed and sent to more than 2,000 experts working with coastal and ocean issues in the Gulf of Mexico to elicit their opinions. The survey asked the experts to rank 12 data categories and score the relevance of four qualifiers (spatial resolution, temporal resolution, age of data, and level of detail) using a discrete choice approach. The statistical interpretation of experts?? responses allowed assigning a relative value to each available data set, based upon their qualifiers. The results were then displayed in a Geographic Information System to visually show how the total value for all data categories or for a specific data category varies spatially and to identify data gaps. It was found that the total data set value is generally higher within Tampa Bay and along the coast. Areas with low total value show a lack of data sets that have qualifiers falling within the ideal range, as per experts?? opinions. The gap maps created for this project are useful to identify priority areas where money should be invested for future data collection.     

Siting MSW landfill using weighted linear combination and analytical hierarchy process (AHP) methodology in GIS environment (case study: Karaj)

Selection of landfill site is a complex process and needs many diverse criteria. The purpose of this paper is to evaluate the suitability of the studied site as landfill for MSW in Karaj. Using weighted linear combination (WLC) method and spatial cluster analysis (SCA), suitable sites for allocation of landfill for a 20-year period were identified. For analyzing spatial auto-correlation of the land suitability map layer (LSML), Maron’s I was used. Finally, using the analytical hierarchy process (AHP), the most preferred alternative for the landfill siting was identified. Main advantages of AHP are: relative ease of handling multiple criteria, easy to understand and effective handling of both qualitative and quantitative data.As a result, 6% of the study area is suitable for landfill siting and third alternative was identified as the most preferred for siting MSW landfill by AHP.The ranking of alternatives were obtained only by applying the WLC approach showed different results from the AHP. The WLC should be used only for the identification of alternatives and the AHP is used for prioritization. We suggest the employed procedure for other similar regions.     

Production and Purification of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Degrading Enzyme from Streptomyces sp. AF-111

A poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) degrading bacterial strain designated as AF-111 was isolated from sewage sludge sample. The bacterium was identified by 16S rRNA gene sequencing. The results revealed that strain AF-111 showed 99 % similarity with Streptomyces althioticus strain NRRL B-3981 and designated as Streptomyces sp. strain AF-111. An extracellular PHBV depolymerase enzyme was produced under optimized conditions and purified through ammonium sulphate fractionation and column chromatography. The enzyme was purified to homogeneity, indicated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and molecular weight was found to be approximately 51 kDa. Effect of temperature, pH, metal ions and inhibitors on the PHBV depolymerase activity was determined. The enzyme was stable at wide range of temperature (35–55 °C) and pH (6–8). PHBV depolymerase was stable in the presence of different metal ions except iron and zinc which had inhibitory effect on depolymerase activity. Both ethylenediamine teteracetic acid and phenylmethyl sulphonyl fluoride strongly inhibited enzyme activity which indicates that this enzyme belongs to the serine hydrolase family like other polyhydroxyalkanoate depolymerases. The results show that a depolymerase from strain AF-111 can effectively degrade PHBV, therefore, it can be applied in the process of biochemical monomer recycling.     

Strategies for the municipal waste management system to take advantage of carbon trading under competing policies: The role of energy from waste in Sydney

Climate change is a driving force behind some recent environmental legislation around the world. Greenhouse gas emission reduction targets have been set in many industrialised countries. A change in current practices of almost all greenhouse-emitting industrial sectors is unavoidable, if the set targets is to be achieved. Although, waste disposal contributes around 3% of the total greenhouse gas emissions in Australia (mainly due to fugitive methane emissions from landfills), the carbon credit and trading scheme set to start in 2010 presents significant challenges and opportunities to municipal solid waste practitioners. Technological advances in waste management, if adopted properly, allow the municipal solid waste sector to act as carbon sink, hence earning tradable carbon credits. However, due to the complexity of the system and its inherent uncertainties, optimizing it for carbon credits may worsen its performance under other criteria. We use an integrated, stochastic multi-criteria decision-making tool that we developed earlier to analyse the carbon credit potential of Sydney municipal solid waste under eleven possible future strategies. We find that the changing legislative environment is likely to make current practices highly non-optimal and increase pressures for a change of waste management strategy.