假单胞菌MBR还原钛(Ⅳ)合成钛纳米粒子

研究了一株假单胞菌MBR(Pseudomonas sp.MBR)在好氧环境下,以有机碳源为电子供体,将氟钛酸盐还原为钛单质的特性及影响还原的因素.结果表明,该菌还原氟钛酸盐初始浓度范围为0.5~8.0 mmol/L,最适pH值为7.0,柠檬酸钠为最佳碳源供给物,并且在电子供体和钛的摩尔比为100:1时还原效果最好.扫描电子显微镜和透射电子显微镜观察显示加氟钛酸盐后细胞的形态并没有发生变化,还原的Ti单质以纳米粒子形式积累在细胞内.     

水文变异下东江流域枯水径流风险率分析

探求人类活动与气候变化对径流枯水风险的影响,对实现区域经济与环境的可持续发展有着重要的意义。以东江流域为例,通过对相对关联距离熵Hd辨识水文系统状态的跳跃,识别水文变异点,采用信息扩散风险分析模型评估径流的枯水风险率,分析径流枯水风险率在水文变异前后的变化趋势及演变情势。研究结果表明：东江流域在1972-1973年发生水文变异,与东江上游枫树坝建设运行时间基本一致。枯水风险率大于0.8的年份皆发生强厄尔尼诺事件,表明了厄尔尼诺现象出现时,当年东江流域月径流量普遍发生减少。在水文变异后,径流的枯水风险率降低,但变化幅度更加剧烈,同时枯水径流的风险率与降雨的相关性减弱,但与降雨的相关性还是超过0.9,这些都揭示了径流在水文变异后在主要受到气候变化影响的同时,越来越受到水利工程的调节作用的影响。研究成果可为流域综合管理部门决策提供参考。     

阜阳城区水体浮游动物周年结构特征及其与水质评价

为研究阜阳城区各水体浮游动物结构特征、多样性水平以及各水体浮游动物的生物量与主要水质指标的相关性,于2011年3月到2012年2月设点采样并进行分析。结果:各水体浮游动物密度有一致特征,一年中分别在4—5月和10月出现高峰,相应密度高峰后一个月左右生物量也出现高峰;与水质检测结果对照,浮游动物密度年平均值大的水域污染明显,相应多样性指数、丰富度指数及均匀度指数都低,而浮游动物密度年均值相对小的水体污染较轻,相应多样性指数、丰富度指数及均匀度指数都高;相关分析表明,浮游动物生物量与相应水体检测指标(CODCr、BOD、DO、TP、NH3-N)显著相关。结论:阜阳城区各水体浮游动物生物量与水体污染程度关系明显,水体水质可凭相应浮游动物生物量来评价,但水生态环境受多种因素的影响,情况复杂,综合分析各层面因素便于得出客观结论。     

Label-free colorimetric nanosensor with improved sensitivity for Pb2+ in water by using a truncated 8–17 DNAzyme

• Unmodified-AuNP based, colorimetric nanosensor was constructed for Pb²⁺ detection. • 5-nucleotide truncation in DNAzyme made complete substrate detachment upon Pb²⁺. • Ultrasensitive and selective detection of Lead (II) was achieved with 0.2×10^-9 mol/L LOD. Water pollution accidents, such as the Flint water crisis in the United States, caused by lead contamination have raised concern on the safety of drinking water distribution systems. Thus, the routine monitoring of lead in water is highly required and demands efficient, sensitive, cost-effective, and reliable lead detection methods. This study reports a label-free colorimetric nanosensor that uses unmodified gold nanoparticles (AuNPs) as indicators to enable rapid and ultra-sensitive detection of lead in environmental water. The 8–17 DNAzyme was truncated in this study to facilitate the detachment of single-stranded DNA fragments after substrate cleavage in the presence of Pb²⁺. The detached fragments were adsorbed over AuNPs and protected against salt concentration-induced aggregation. Accordingly, high Pb²⁺ would result in rapid color change from blue to pink. The established sensing principle achieved a sensitive limit of detection of 0.2×10^-9 mol/L Pb²⁺, with a linear working range of two orders of magnitude from 0.5×10^-9 mol/L to 5×10^-9 mol/L. The selectivity of the nanosensor was demonstrated by evaluating the interfering metal ions. The developed nanosensor can serve as a substitute for the rapid analysis and monitoring of trace lead levels under the drinking water distribution system and even other environmental water samples.     

草甘膦对GC-1小鼠精原细胞的毒性作用及NAC的干预效应

为探讨41%草甘膦水溶液(农达)对雄性生殖细胞的毒性及其作用机制以及N-乙酰半胱氨酸(N-acetylcysteine, NAC)的干预效应。以GC-1小鼠精原细胞为受试细胞,设正常对照组、草甘膦染毒组(60、90、120、150、180 mg·L^-1)、NAC干预组(10 mmol·L^-1 NAC＋90 mg·L^-1农达)。MTT法检测细胞存活率,Giemsa染色法观察细胞的形态学改变,彗星试验检测细胞DNA损伤,比色法检测细胞培养液乳酸脱氢酶(LDH)以及细胞内超氧化物歧化酶(SOD)、谷胱甘肽(GSH)及丙二醛(MDA)水平的变化。结果显示,随着草甘膦染毒浓度增加,细胞存活率逐渐下降(p<0.01),彗星阳性率逐渐升高(p<0.01);与对照组相比,草甘膦染毒组LDH活性增加(p<0.05,60 mg·L^-1组除外),MDA生成量增多(p<0.05),GSH含量降低(p<0.05)和SOD活性降低(p<0.05)。抗氧化剂NAC预处理具有相应的拮抗作用。研究表明,60～180 mg·L^-1浓度草甘膦对GC-1细胞有明显的损伤作用,其机制可能是草甘膦诱导氧化应激,导致细胞通透性增加和DNA损伤。抗氧化剂NAC对草甘膦的细胞毒性具有一定保护作用。     

Simultaneous removal of arsenate and fluoride from water by AI-Fe （hydr）oxides

A1-Fe （hydr）oxides with different A1/Fe molar ratios （4：1, 1：1, 1：4, 0：1） were prepared using a co- precipitation method and were then employed for simultaneous removal of arsenate and fluoride. The 4A1 ： Fe was superior to other adsorbents for removal of arsenate and fluoride in the pH range of 5.0-9.0. The adsorption capacity of the A1-Fe （hydr）oxides for arsenate and fluoride at pH 6.50.3 increased with increasing A1 content in the adsorbents. The linear relationship between the amount of OH released from the adsorbent and the amount of arsenate or fluoride adsorbent by 4A1 ： Fe indicated that the adsorption of arsenate and fluoride by A1- Fe （hydr）oxides was realized primarily through quantita- tive ligand exchange. Moreover, there was a very good correlation between the surface hydroxyl group densities of A1-Fe （hydr）oxides and their adsorption capacities for arsenate or fluoride. The highest adsorption capacity for arsenate and fluoride by 4A1 ： Fe is mainly ascribed to its highest surface hydroxyl group density besides its largest pHpzc. The dosage of adsorbent necessary to remove arsenate and fluoride to meet the drinking water standard was mainly determined by the presence of fluoride since fluoride was generally present in groundwater at much higher concentration than arsenate.     

磺胺甲恶唑污染对颤蚓的氧化胁迫

在实验室模拟条件下分别采用动、静态生物实验方法,研究了不同浓度的磺胺甲恶唑(sulfamethoxazole,SMZ)对颤蚓(Monopyle phorus limosus)体内抗氧化防御系统的影响以及颤蚓暴露于单一浓度的SMZ后,其体内典型的抗氧化酶,超氧化物歧化酶(SOD)的活性随时间的响应。结果显示:染毒8d后,颤蚓体内SOD的活性先降低后逐渐升高。SMZ染毒浓度达到1.00mg·L-1时,SOD的活性被显著诱导(P<0.01),为对照组的1.30倍。过氧化物酶(POD)活性的变化趋势明显分为先升高、再降低2个阶段。染毒浓度达到0.25mg·L-1时,POD的活性受到最大诱导(P<0.01),约为对照组的1.40倍。各处理组过氧化氢酶(CAT)的活性均高于对照组,并且随着SMZ暴露浓度的增加,CAT活性的变化趋势是逐渐降低的,与SMZ染毒浓度间存在较好的剂量-效应关系。随着SMZ暴露时间的延长,SOD、POD和CAT的活性变化趋势类似,均为先升高后降低,并在第8天时达到最大值(P<0.01),增长率分别为32%、26%和82%。综上,SMZ能诱导颤蚓体内产生氧化胁迫,而CAT在活性氧自由基的清除中发挥主要作用。     

Poverty issues in a national wildlife reserve in China

The increasing interest in environmental conservation is a global phenomenon. In many countries, natural reserves or protected areas (PAs) have been established to restrict human activities and to enhance natural ecosystems, which potentially benefit the planet as a whole. However, this often demands some sacrifices from local residents who may have to give up resources they rely on or suffer losses caused by wildlife. This issue potentially becomes aggravated in densely populated areas where many people and economic activities are impacted. This paper presents research findings on poverty issues in a newly established national natural reserve in China, Hengshui Lake Natural Reserve. The paper addresses the characteristics of poor households in the reserve and the interaction between these families and the reserve. In addition, poverty alleviation measures are analysed and additional measures to reduce poverty and conflicts between the reserve and local residents are recommended.     

黑龙江省霜冻的气候变化特征和预报指标

利用1970-2008年地面最低气温和历史天气图资料,对黑龙江省初、终霜冻的天气气候特征进行了分析,并探讨了出现霜冻的环流形势特点,总结出黑龙江省出现霜冻的三种天气学模型:①超极地路径型;②西方路径型;③西北路径型。给出了霜冻的时空分布特征以及对预报有用的一些预报指标,对霜冻预报业务有参考作用。     

农牧交错带土地利用格局演变研究进展

农牧交错带作为我国农业与牧业区交汇的桥梁和纽带,其生态系统突变和变化方向的不确定性日益突出。在总结农牧交错带自然条件、存在问题和发展现状的基础上重点梳理了土地利用格局驱动力及其演变对生态过程耦合和区域生态环境的影响;基于生态化学计量学元素平衡与循环机制研究土地利用格局演变规律的研究进展,最后对目前基础理论和研究手段进行了分析和前景展望。