Heavy metal ion influence on the photosynthetic growth of Rhodobacter sphaeroides

The potential of purple non-sulphur bacteria for bioremediation was assessed by investigating the ability of Rhodobacter sphaeroides strain R26.1 to grow photosynthetically in heavy metal contaminated environments. Bacterial cultures were carried out in artificially polluted media, enriched with the transition metal ions Hg2+, Cu2+, Fe2+, Ni2+, Co2+, MoO4(2-), and CrO4(2-) in millimolar concentration range. For each investigated ion the effect on growth parameters was evaluated. The analysis of concentration-effect curves revealed a differentiated response, indicating that diverse mechanisms of tolerance and/or resistance are involved. Adaptation or selection procedures were not applied, leading to assess intrinsic abilities of coping with these contaminants. The microorganism proved to be highly tolerant to heavy metal exposure, especially towards Co2+, Fe2+ and MoO4(2-). In addition Ni2+ and Co2+ were found to decrease the cellular content of light harvesting complexes. A characteristic behavior was observed with mercuric ions, which produced a significant increase of the lag-phase.     

Belligerent broadcasting,male anti-authoritarianism and anti-environmentalism: the case of Top Gear (BBC, 2002–2015)

ABSTRACT

This article considers the format and cultural politics of the hugely successful UK television program Top Gear (BBC 2002–2015). It analyzes how—through its presenting team—it constructed an informal address predicated around anti-authoritarian or contrarian banter and protest masculinity. Regular targets for Top Gear presenter’s protest—curtailed by broadcast guidelines in terms of gender and ethnicity—are deflected onto the “soft” targets of government legislation on environmental issues or various forms of regulation “red tape. Repeated references to speed cameras, central London congestion charges and “excessive” signage are all anti-authoritarian, libertarian discourses delivered through a comedic form of performance address. Thus, the BBC’s primary response to complaints made about this program was to defend the program’s political views as being part of the humor. The article draws on critical discourse analysis and conversation analysis to consider how the program licensed a particular form of engagement that helped it to deflect criticisms, and considers the limits to such discursive positioning. We conclude by examining the controversies that finally led, in 2015, to the removal of the main presenter, Jeremy Clarkson, and the ending of this version of the program through the departure of the team to an on-demand online television service.     

A dynamic optimization model for solid waste recycling

Recycling is an important part of waste management (that includes different kinds of issues: environmental, technological, economic, legislative, social, etc.). Differently from many works in literature, this paper is focused on recycling management and on the dynamic optimization of materials collection. The developed dynamic decision model is characterized by state variables, corresponding to the quantity of waste in each bin per each day, and control variables determining the quantity of material that is collected in the area each day and the routes for collecting vehicles. The objective function minimizes the sum of costs minus benefits. The developed decision model is integrated in a GIS-based Decision Support System (DSS). A case study related to the Cogoleto municipality is presented to show the effectiveness of the proposed model. From optimal results, it has been found that the net benefits of the optimized collection are about 2.5 times greater than the estimated current policy.     

Dust explosion incidents and regulations in the United States

The U.S. Chemical Safety and Hazard Investigation Board (CSB) investigated three fatal dust explosions that all occurred in 2003. These explosions caused the deaths of 14 people and injured hundreds more. Two of the facilities were damaged beyond repair, and several hundred employees lost their jobs.

CSB's investigations revealed that the explosions had common causes, despite their geographic and industrial diversity. Consequently, CSB commissioned a study of combustible dust fire and explosion hazards. This paper presents a summary of CSB's findings and recommendations developed during that study.     

Intriguing olfactory proteins from the yellow fever mosquito,<Emphasis Type="Italic"> Aedes aegypti</Emphasis>

Four antennae-specific proteins (AaegOBP1, AaegOBP2, AaegOBP3, and AaegASP1) were isolated from the yellow fever mosquito, Aedes aegypti and their full-length cDNAs were cloned. RT-PCR indicated that they are expressed in female and, to a lesser extent, in male antennae, but not in control tissues (legs). AaegOBP1 and AaegOBP3 showed significant similarity to previously identified mosquito odorant-binding proteins (OBPs) in cysteine spacing pattern and sequence. Two of the isolated proteins have a total of eight cysteine residues. The similarity of the spacing pattern of the cysteine residues and amino acid sequence to those of previously identified olfactory proteins suggests that one of the cysteine-rich proteins (AaegOBP2) is an OBP. The other (AaegASP1) did not belong to any group of known OBPs. Structural analyses indicate that six of the cysteine residues in AaegOBP2 are linked in a similar pattern to the previously known cysteine pairing in OBPs, i.e., Cys-24–Cys-55, Cys-51–Cys-104, Cys-95–Cys-113. The additional disulfide bridge, Cys-38–Cys-125, knits the extended C-terminal segment of the protein to a predicted 2-helix. As indicated by circular dichroism (CD) spectra, the extra rigidity seems to prevent the predicted formation of a C-terminal -helix at low pH.Electronic Supplementary Material  Supplementary material is available for this article if you access the article at . A link in the frame on the left on that page takes you directly to the supplementary material.     

Overfill protective systems – Complex problem,simple solution

Overfills have resulted in significant process safety incidents. Longford (Australia, 1998), Texas City (United States, 2005), and Buncefield (United Kingdom, 2005) can be traced to loss of level control leading to high level and ultimately to loss of containment. A tower at Longford and a fractionating column at Texas City were overfilled, allowing liquid to pass to downstream equipment that was not designed to receive it. The Buncefield incident occurred when a terminal tank was overfilled releasing hydrocarbons through its conservation vents.The causes of overfill are easy to identify; however, the risk analysis is complicated by the combination of manual and automated actions often necessary to control level and to respond to abnormal level events. This paper provides a brief summary of the Longford, Texas City, and Buncefield incidents from an overfill perspective and highlights 5 common factors that contributed to making these incidents possible. Fortunately, while overfill can be a complex problem, the risk reduction strategy is surprisingly simple.     

Spatial variability of air pollutants in the city of Taranto, Italy and its potential impact on exposure assessment

Epidemiological studies typically use monitored air pollution data from a single station or as averaged data from several stations to estimate population exposure. In industrialized urban areas, this approach may present critical issues due to the spatial complexities of air pollutants which are emitted by different sources. This study focused on the city of Taranto, which is one of the most highly industrialized cities in southern Italy. Epidemiological studies have revealed several critical situations in this area, in terms of mortality excess and short-term health effects of air pollution. The aims of this paper are to study the variability of air pollutants in the city of Taranto and to interpret the results in relation to the applicability of the data in assessing population exposure. Meteorological and pollution data (SO2, NO2, PM10), measured simultaneously and continuously during the period 2006–2010 in five air quality stations, were analyzed. Relative and absolute spatial concentration variations were investigated by means of statistical indexes. Results show significant differences among stations. The highest correlation between stations was observed for PM10 concentrations, while critical values were found for NO2. The worst values were observed for the SO2 series. The high values of 90th percentile of differences between pairs of monitoring sites for the three pollutants index suggest that mean concentrations differ by large amounts from site to site. The overall analysis supports the hypothesis that various parts of the city are differently affected by the different emission sources, depending on meteorological conditions. In particular, analysis revealed that the influence of the industrial site may be primarily identified with the series of SO2 data which exhibit higher mean concentration values and positive correlations with wind intensity when the monitoring station is downwind from the industrial site. Results suggest evaluating the population exposure to air pollutants in industrialized cities by taking into account the possible zones of influence of different emission sources. More research is needed to identify an indicator, which ought to be a synthesis of several pollutants, and take into account the meteorological variables.     

Environmental drivers of decadal change of a mangrove forest in the North coast of the Yucatan peninsula,Mexico

Mangrove forests provide important ecosystem services, but are under constant pressure from natural, anthropogenic, and climate change related disturbances. Environmental drivers on mangrove change at large spatial scales, other than sea level rise, are not well understood. In here, we use spatially explicit methods to identify the main environmental drivers of mangrove coverage change over a decade in the landscape of the North coast of the Yucatan peninsula, Mexico. A post-supervised classification approach on seven SPOT 5 multispectral satellite images was used to construct thematic maps of mangrove coverage between 2004 and 2014. A linear regression model between the thematic maps was performed to estimate the mangrove coverage change rate per pixel. Climate surfaces for annual maximum, minimum and mean temperature, and annual mean and cumulative precipitation for the region were calculated for the period 1980–2009 using data obtained from the National Meteorological Service. The effect of environmental variables on mangrove coverage change rates was assessed with a boosted generalized additive model (boosted GAM). The lowest and highest overall accuracy obtained for the time series thematic maps were 87.14% (Kappa?=?0.78), and 97.5% (Kappa?=?0.95), respectively. The most influential environmental variables on mangrove coverage change were annual cumulative precipitation (21%), and annual maximum temperature (9%). Current climate change scenarios for the region predict an increase in temperature and a decrease in precipitation, intensifying environmental stress on this ecosystem. Therefore, adequate management strategies are fundamental to help maintain the mangrove forest under changing environmental conditions.     

Are Toronto’s streams sick? A look at the fish and benthic invertebrate communities in the Toronto region in relation to the urban stream syndrome

Impacts of urbanization on aquatic ecosystems are intensifying as urban sprawl spreads across the global land base. The urban stream syndrome (USS) identifies “symptoms” associated with urban development including changes in biotic communities, hydrology, water chemistry, and channel morphology. Direct relationships between road density (as surrogate of urbanization) and indicators of the USS were identified for streams in the Toronto region. Significant negative relationships were revealed between road density and biological (fish and benthic macroinvertebrate) richness, diversity, and fish Index of Biotic Integrity scores. Significant positive relationships were found between road density and tolerant fish/benthic macroinvertbrates, benthos Family Biotic Index scores, mean summer stream temperature, stream flashiness, and several water quality variables. Analysis of biological data showed that only four fish species and a reduced number of benthic macroinvertebrate families remained at the most urbanized sites. Road density was found to be a major determinant in both the fish and benthic macroinvertebrate community structure.