不同碳源条件下草菇内切型纤维素酶基因(egl)转录表达的分析

利用Northern blot方法分析了不同碳源条件下草菇內切型纤维素酶基因(eg1)的表达.结果发现,在含有纤维素的液体培养基中生长10 d,eg1在草菇菌丝中有高效表达;纤维二糖、α-乳糖、β-乳糖,也能诱导eg1的表达,但和纤维素相比,eg1的表达量相对较低,并且它们的诱导效应在加入这类糖12 h后迅速减弱;槐糖和龙胆二糖的诱导作用非常弱.在天然稻草为基质的固体栽培料生长时,草菇eg1的表达和草菇菌丝生长与出菇相对应,在菌丝生长期(d 8)可见eg1的表达,d 12时菌丝已长满,表达减弱,在出菇及菇体的分化及增大期,eg1的表达量逐渐增强,在成熟期达到最高水平;表明在草菇菇体发育中需要更多碳源及能源的补充,eg1在这方面起着非常重要的作用. 图4 参14     

Material dispersion by oceanic internal waves

Internal gravity waves that are generated in the open ocean have a universal frequency spectrum, called Garrett–Munk spectrum. By initializing internal waves that satisfy the Garrett–Munk spectrum in a non-hydrostatic numerical model, we investigate the material dispersion produced by these internal waves. Three numerical experiments are designed: Exp.-1 uses a linearly stratified fluid, Exp.-2 has an upper mixed layer, and Exp.-3 incorporates a circular front into the upper mixed layer. Resorting to neutrally buoyant particles, we investigate the dispersion in terms of metrics of the relative dispersion and finite-scale Lyapunov exponent (FSLE). Exp.-1 shows that the dispersion regime produced by these internal waves is between ballistic and diffusive based on relative dispersion, and is however ballistic according to FSLE. The maximum FSLE at scales of 100 m is about 5 day\(^{-1}\), which is comparable to that calculated using ocean drifters. Exp.-2 demonstrates that internal waves can generate flows and material dispersion in an upper mixed layer. However, when mixed layer eddies are present, as in Exp.-3, the dispersion in the mixed layer is controlled by the eddies. In addition, we show that inertial oscillations do not affect the relative dispersion, but impact FSLE at scales of inertial oscillations.