Biodiversity of the ground-dwelling spider fauna of afforestation habitats

The aim of this study was to assess spider diversity among habitats that are typically used for afforestation in Ireland, and to identify habitat parameters which could potentially be used as indicators of their biodiversity value. Ground-dwelling spiders were surveyed in 24 sites across Ireland, with eight sites of each of the following habitat types: improved grassland, wet grassland and peatland. The spiders were sampled using pitfall traps which were located within the major vegetation types present in each site as well as within supplementary habitat features which may add to biodiversity value of the whole site such as hedgerows, flushes and the edges of ditches and streams.Each habitat supported distinct spider assemblages that reflect major differences in both environmental conditions and management regime. The improved grasslands had low spider species richness and low variation in assemblage structure which is probably related to the intensive management of this habitat. In this case hedgerows maybe an important aspect of the spider diversity within agricultural landscapes. The peatlands, and to a lesser extent wet grasslands, supported a diverse and specialist spider fauna, including a number of rare species; this may be due to differences in soil moisture and plant architecture. Indicators of biodiversity value identified included wet flushes in the peatlands and low grazing pressure in the wet grasslands. This study suggests that in terms of biodiversity value improved grassland is the preferable habitat for afforestation, because of the poor baseline spider diversity. However, it may be unrealistic to expect land owners to afforest their most productive agricultural land, so the management and habitat indicators identified in this study may be of use for assessing habitat quality among the wet grassland and peatlands to allow sites with lower biodiversity value to be identified.     

Isotopic evidence of distinct feeding ecologies and movement patterns in two migratory predators (yellowfin tuna and swordfish) of the western Indian Ocean

Ecologists primarily use δ¹⁵N values to estimate the trophic level of organisms, while δ¹³C, and even recently δ¹⁵N, are utilized to delineate feeding habitats. However, many factors can influence the stable isotopic composition of consumers, e.g. age, starvation or isotopic signature of primary producers. Such sources of variability make the interpretation of stable isotope data rather complex. To examine these potential sources of variability, muscle tissues of yellowfin tuna (Thunnus albacares) and swordfish (Xiphias gladius) of various body lengths were sampled between 2001 and 2004 in the western Indian Ocean during different seasons and along a latitudinal gradient (23°S to 5°N). Body length and latitude effects on δ¹⁵N and δ¹³C were investigated using linear models. Both latitude and body length significantly affect the stable isotope values of the studied species but variations were much more pronounced for δ¹⁵N. We explain the latitudinal effect by differences in nitrogen dynamics existing at the base of the food web and propagating along the food chain up to top predators. This spatial pattern suggests that yellowfin and swordfish populations exhibit a relatively unexpected resident behaviour at the temporal scale of their muscle tissue turnover. The body length effect is significant for both species but this effect is more pronounced in swordfish as a consequence of their different feeding strategies, reflecting specific physiological abilities. Swordfish adults are able to reach very deep water and have access to a larger size range of prey than yellowfin tuna. In contrast, yellowfin juveniles and adults spend most of their time in the surface waters and large yellowfin tuna continue to prey on small organisms. Consequently, nitrogen isotopic signatures of swordfish tissues are higher than those of yellowfin tuna and provide evidence for different trophic levels between these species. Thus, in contrast to δ¹³C, δ¹⁵N analyses of tropical Indian Ocean marine predators allow the investigation of complex vertical and spatial segregation, both within and between species, even in the case of highly opportunistic feeding behaviours. The linear models developed in this study allow us to make predictions of δ¹⁵N values and to correct for any body length or latitude differences in future food web studies.     

Prevalence of Human Noroviruses in Frozen Marketed Shellfish,Red Fruits and Fresh Vegetables

Noroviruses (NoVs), currently recognised as the most common human food-borne pathogens, are ubiquitous in the environment and can be transmitted to humans through multiple foodstuffs. In this study, we evaluated the prevalence of human NoV genogroups I (GI) and II (GII) in 493 food samples including soft red fruits (n = 200), salad vegetables (n = 210) and bivalve mollusc shellfish (n = 83), using the Bovine Enterovirus type 1 as process extraction control for the first time. Viral extractions were performed by elution concentration and genome detection by TaqMan Real-Time RT-PCR (RT-qPCR). Experimental contamination using hepatitis A virus (HAV) was used to determine the limit of detection (LOD) of the extraction methods. Positive detections were obtained from 2 g of digestive tissues of oysters or mussels kept for 16 h in seawater containing 2.0–2.7 log₁₀ plaque-forming units (PFU)/L of HAV. For lettuces and raspberries, the LOD was, respectively, estimated at 2.2 and 2.9 log₁₀ PFU per 25 g. Of the molluscs tested, 8.4 and 14.4 % were, respectively, positive for the presence of GI NoV and GII NoV RNA. Prevalence in GI NoVs varied from 11.9 % for the salad vegetables samples to 15.5 % for the red soft fruits. Only 0.5 % of the salad and red soft fruits samples were positive for GII NoVs. These results highlight the high occurrence of human NoVs in foodstuffs that can be eaten raw or after a moderate technological processing or treatment. The determination of the risk of infection associated with an RT-qPCR positive sample remains an important challenge for the future.     

Adaptation to climate change: changes in farmland use and stocking rate in the U.S.

This paper examines possible adaptations to climate change in terms of pasture and crop land use and stocking rate in the United States (U.S.). Using Agricultural Census and climate data in a statistical model, we find that as temperature and precipitation increases agricultural commodity producers respond by reducing crop land and increasing pasture land. In addition, cattle stocking rate decreases as the summer Temperature-humidity Index (THI) increases and summer precipitation decreases. Using the statistical model with climate data from four General Circulation Models (GCMs), we project that land use shifts from cropping to grazing and the stocking rate declines, and these adaptations are more pronounced in the central and the southeast regions of the U.S. Controlling for other farm production variables, crop land decreases by 6 % and pasture land increases by 33 % from the baseline. Correspondingly, the associated economic impact due to adaptation is around ?14 and 29 million dollars to crop producers and pasture producers by the end of this century, respectively. The national and regional results have implications for farm programs and subsidy policies.     

Genetic Analysis of Norovirus Strains that Caused Gastroenteritis Outbreaks Among River Rafters in the Grand Canyon,Arizona

Toilet solid waste samples collected from five outbreaks among rafters in the Grand Canyon were subjected to sequencing analysis of norovirus partial capsid gene. The results revealed that a GI.3 strain was associated with one outbreak, whereas the other outbreaks were caused by GII.5 whose sequences shared >98.9% homology.     

Phosphogypsum capping depth affects revegetation and hydrology in Western Canada

Phosphogypsum (PG), a byproduct of phosphate fertilizer manufacturing, is commonly stacked and capped with soil at decommissioning. Shallow (0, 8, 15, and 30 cm) and thick (46 and 91 cm) sandy loam caps on a PG stack near Fort Saskatchewan, Alberta, Canada, were studied in relation to vegetation establishment and hydrologic properties. Plant response was evaluated over two growing seasons for redtop ( L.), slender wheatgrass ( (Link) Malte ex H.F. Lewis), tufted hairgrass ( (L.) P. Beauv.), and sheep fescue ( L.) and for a mix of these grasses with alsike clover ( L.). Water content below the soil-PG interface was monitored with time-domain reflectometry probes, and leachate water quantity and quality at a depth of 30 cm was measured using lysimeters. Vegetation responded positively to all cap depths relative to bare PG, with few significant differences among cap depths. Slender wheatgrass performed best, and tufted hairgrass performed poorly. Soil caps <1 m required by regulation were sufficient for early revegetation. Soil water fluctuated more in shallow than in thick caps, and water content was generally between field capacity and wilting point regardless of cap depth. Water quality was not affected by cap depths ≤30 cm. Leachate volumes at 30 cm from distinct rainfall events were independent of precipitation amount and cap depth. The study period had lower precipitation than normal, yet soil caps were hospitable for plant growth in the first 2 yr of establishment.