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171.
Francine Piubeli Matthew J. Grossman Fabiana Fantinatti-Garboggini Lucia R. Durrant 《Environmental science and pollution research international》2014,21(20):12006-12016
In this work the archaea and eubacteria community of a hypersaline produced water from the Campos Basin that had been transported and discharged to an onshore storage facility was evaluated by 16S recombinant RNA (rRNA) gene sequence analysis. The produced water had a hypersaline salt content of 10 (w/v), had a carbon oxygen demand (COD) of 4,300 mg/l and contains phenol and other aromatic compounds. The high salt and COD content and the presence of toxic phenolic compounds present a problem for conventional discharge to open seawater. In previous studies, we demonstrated that the COD and phenolic content could be largely removed under aerobic conditions, without dilution, by either addition of phenol degrading Haloarchaea or the addition of nutrients alone. In this study our goal was to characterize the microbial community to gain further insight into the persistence of reservoir community members in the produced water and the potential for bioremediation of COD and toxic contaminants. Members of the archaea community were consistent with previously identified communities from mesothermic reservoirs. All identified archaea were located within the phylum Euryarchaeota, with 98 % being identified as methanogens while 2 % could not be affiliated with any known genus. Of the identified archaea, 37 % were identified as members of the strictly carbon-dioxide-reducing genus Methanoplanus and 59 % as members of the acetoclastic genus Methanosaeta. No Haloarchaea were detected, consistent with the need to add these organisms for COD and aromatic removal. Marinobacter and Halomonas dominated the eubacterial community. The presence of these genera is consistent with the ability to stimulate COD and aromatic removal with nutrient addition. In addition, anaerobic members of the phyla Thermotogae, Firmicutes, and unclassified eubacteria were identified and may represent reservoir organisms associated with the conversion hydrocarbons to methane. 相似文献
172.
Ecotoxicological assessment of metal-polluted urban soils using bioassays with three soil invertebrates 总被引:1,自引:0,他引:1
This study aimed at assessing the quality of urban soils by integrating chemical and ecotoxicological approaches. Soils from five sites in downtown Naples, Italy, were sampled and characterized for physical-chemical properties and total and water-extractable metal concentrations. Bioassays with Eisenia andrei, Enchytraeus crypticus and Folsomia candida were performed to assess toxicity of the soils, using survival, reproduction and growth as the endpoints. Metal bioaccumulation in the animals was also measured. The properties and metal concentrations of the soils strongly differed. Metal bioaccumulation was related with total metal concentrations in soil and was highest in E. crypticus, which was more sensitive than E. andrei and F. candida. Responses of the three species to the investigated soils seemed due to both metal contamination and soil properties. 相似文献
173.
Zubcov E Zubcov N Ene A Biletchi L 《Environmental science and pollution research international》2012,19(6):2238-2247
Purpose
The aim of this work was to assess the levels of copper and zinc in fish from the main freshwater ecosystems of Moldova, in relation with species, habitat, age, sex, season, and development stage.Methods
Fish from Cyprinidae and Percidae families (Cyprinus carpio, Carassius auratus gibelio, Rutilus rutilus heckeli, Abramis brama, Aristichthys nobilis, Hypophtalmichthys molitrix, Sander lucioperca) were collected from Prut and Dniester rivers, Cuciurgan, Dubasari, and Costesti-Stanca reservoirs, and ponds of farms in the Dniester delta. The Cu and Zn content of fish tissues (skeletal muscles, liver, gonads, gills, skin, and scales) was determined by flame atomic absorption spectrophotometer AAS-3, of water by graphite furnace HGA 900 of AAnalist 400.Results
The level of heavy metals accumulation in muscles of immature fish follows their dynamics in water. The highest concentration of zinc was registered in the gonads of mature fish, and of copper??in the liver. The lowest Cu and Zn contents were recorded in the muscles and are in the United Nations Food and Agriculture Organization safety-permissible levels for human consumption. Cu and Zn contents in muscles of fish depend on specimen age. Their level in fish gonads was sharply increasing during pre-spawning period. During the early developmental stages, the metal concentration in fish eggs and larvae varies within wide limits, but the accumulation pattern is similar in the investigated species.Conclusions
The fish represent one of the most indicative factors for the estimation of trace metals pollution in freshwater systems and this is important not only for monitoring purposes, but also for the fish culture ones. 相似文献174.
Ciniglia C Sansone C Panzella L Napolitano A d'Ischia M 《Environmental science and pollution research international》2012,19(8):3299-3306
INTRODUCTION: The reuse of wastewaters for agricultural purposes is a common practice in many countries and is increasingly recommended by organizations that promote sustainable development. Yet, it is restricted by the potential negative impact of these materials on soil and crops. The aim of this study was therefore to evaluate the environmental impact of walnut husk washing waters (WHWW) and their organic fractions, in order to conceive their agricultural exploitation. DISCUSSION: Phytotoxicity tests and morphological investigations on representative plant species of horticultural interest indicated that WHWW and their organic fractions can elicit a concentration-dependent stimulating effect on the growth of radish, lettuce cv. cavolo Napoli with effects up to 165 %. An opposite inhibitory effect up to 70 % was observed on spinach and lettuce cv. Gentilina. Proapoptotic effects were observed by acridine orange/ethidium bromide assay in the species inhibited by WHWW treatment. High-performance liquid chromatography-mass spectrometry analysis of the WHWW revealed the presence of a main component which was extracted selectively in organic solvents and purified by preparative chromatography. Complete spectral analysis allowed identification as 4,8-dihydroxy-1-tetralone, commonly known as regiolone. Regiolone exhibited the same concentration-dependent activity on root elongation with a stimulation in the case of radish up to 135 % with respect to control. These results open perspectives in the exploitation of WHWW and the main phenolic constituent readily available by a straightforward isolation procedure as a natural fertilizer for specific crops. 相似文献
175.
Noortje M. Reeuwijk Antonia Talidda Rainer Malisch Alexander Kotz Angelika Tritscher Heidelore Fiedler Marco J. Zeilmaker Martin Kooijman Koen J.H. Wienk Wim A. Traag Ron L.A.P. Hoogenboom 《Chemosphere》2013,90(5):1678-1685
Geophagy, the practice of consuming clay or soil, is encountered among pregnant women in Africa, Eastern Asia and Latin America, but also in Western societies. However, certain types of clay are known to contain high concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs). The aim of this study was to determine the PCDD/F contents of orally consumed clays purchased from Dutch and African markets. Congener patterns were compared with those of pooled human milk samples collected in eight African countries, to investigate a possible relationship with clay consumption. From the Dutch market thirteen clay products were examined, seven of African and six of Suriname origin. From seven African countries, twenty clay products were collected. All 33 clay products were screened with a cell-based bioassay and those showing a high response were analyzed by GC/HRMS. High PCDD/F concentrations were measured in three clay products from the Dutch market, ranging from 66 to 103 pg TEQ g-1, whereas clay products from African countries were from 24 to 75 pg TEQ g-1. Patterns and relatively high concentrations of PCDD/Fs in human milk samples from the Democratic Republic of the Congo and Côte d’Ivoire suggest a relationship with the consumption of contaminated clay. Frequent use of PCDD/F contaminated clay products during pregnancy may result in increased exposure of the mother and subsequently the developing fetus and new-born child. The use of these contaminated clays during pregnancy should be carefully considered or even discouraged. 相似文献
176.
Antonia Engert Shumon Chakrabarti Nadine Saul Michal Bittner Ralph Menzel Christian E.W. Steinberg 《Chemosphere》2013,90(7):2136-2141
For organisms, temperature is one of the most important environmental factors and gains increasing importance due to global warming, since increasing temperatures may pose organisms close to their environmental tolerance limits and, thus, they may become more vulnerable to environmental stressors. We analyzed the temperature-dependence of the water-soluble antioxidant capacity of the cladoceran Moina macrocopa and evaluated its life trait variables with temperature (15, 20, 25, 30 °C) and humic substance (HS) concentrations (0, 0.18, 0.36, 0.90, 1.79 mM DOC) as stressors. Temperatures below and above the apparent optimum (20 °C) reduced the antioxidative capacity. Additions of HSs increased body length, but decreased mean lifespan at 15 and 20 °C. There was no clear HS-effect on offspring numbers at 15, 20, and 30 °C. At 25 °C with increasing HS-concentration, lifespan was extended and offspring numbers increased tremendously, reaching 250% of the control. Although the applied HS preparation possesses estrogenic and antiandrogenic activities, a xenohormone mechanism does not seem plausible for the reproductive increase, because comparable effects did not occur at other temperatures. A more convincing explanation appears to be the mitohormesis hypothesis which states that a certain increase of reactive oxygen production leads to improved health and longevity and, with Moina, also to increased offspring numbers. Our results suggest that at least with the eurythermic M. macrocopa, a temperature above the optimum can be beneficial for several life trait variables, even when combined with a chemical stressor. Temperatures approximately 10 °C above its optimum appear to adversely affect the lifespan and reproduction of M. macrocopa. This indicates that this cladoceran species seems to be able to utilize temperature as an ecological resource in a range slightly above its thermal optimum. 相似文献
177.
Lucia Barra Maria Valeria Ruggiero Diana Sarno Marina Montresor Wiebe H. C. F. Kooistra 《Environmental science and pollution research international》2013,20(10):6705-6718
The planktonic diatom genus Pseudo-nitzschia contains several genetically closely related species. Some of these can produce domoic acid, a potent neurotoxin. Thus, monitoring programs are needed to screen for the presence of these toxic species. Unfortunately, many are impossible to distinguish using light microscopy. Therefore, we assessed the applicability of microarray technology for detection of toxic and non-toxic Pseudo-nitzschia species in the Gulf of Naples (Mediterranean Sea). Here, 11 species have been detected, of which at least 5 are potentially toxic. A total of 49 genus- and species-specific DNA probes were designed in silico against the nuclear LSU and SSU rRNA of 19 species, and spotted on the microarray. The microarray was tested against total RNA of monoclonal cultures of eight species. Only three of the probes designed to be species-specific were indeed so within the limits of our experimental design. To assess the effectiveness of the microarray in detecting Pseudo-nitzschia species in environmental samples, we hybridized total RNA extracted from 11 seasonal plankton samples against microarray slides and compared the observed pattern with plankton counts in light microscopy and with expected hybridization patterns obtained with monoclonal cultures of the observed species. Presence of species in field samples generally resulted in signal patterns on the microarray as observed with RNA extracted from cultures of these species, but many a-specific signals appeared as well. Possible reasons for the numerous cross reactions are discussed. Calibration curves for Pseudo-nitzschia multistriata showed linear relationship between signal strength and cell number. 相似文献
178.
Tommaso Chiti Giacomo Certini Lucia Perugini Giovanni Mastrolonardo Dario Papale Riccardo Valentini 《Regional Environmental Change》2011,11(2):371-376
The purpose of the present work is to asses the possibility of detecting changes in soil organic carbon (SOC) at the end of
the 5-years of the first Commitment Period (CP) of the Kyoto Protocol of the United Nation’s Framework Convention on Climate
Change (1 January, 2008–31 December, 2012), by both direct measurement and the use of an opportunely evaluated SOC model,
CENTURY. The investigated soil is young, developed since 28 years on virtually C-free spoil banks and under the influence
of two managed forest stands, one a mix of English oak (Quercus robur L.) and Italian alder (Alnus cordata Loisel.) and the other pure English oak. The SOC stock of either stand was monitored since the time the stands were planted
in 1981, and it was used together with other parameters for the model evaluation, while the future projections for the end
of the first (2012) and second (2017) CP were made according to two extreme IPCC climatic scenarios: A1F1, the most dramatic,
and B2, among the less impacting. Direct SOC measurements performed at the beginning and at the end of a time frame equivalent
to a commitment period (2004–2008) had not shown significant variations in either stands. Compared to the 2008 SOC stock,
in both stands the model shows variations at the end of the first CP from 0.7 to 1.8 Mg C ha−1 for the A1F1 scenario and from 0.3 to 1.7 Mg C ha−1 for the B2. These variations are within the standard deviations of the C stocks measured in 2008. On the contrary, at the
end of the second CP, the modelled SOC increments range from 2.5 to 3.6 Mg C ha−1 (A1F1) or from 1.9 to 3.4 Mg C ha−1 (B2), indicating the possibility to detect the SOC changes by direct measurement, since the values well agree with the minimum
detectable variation estimated for both sites in 3.3–4.5 Mg C ha−1. This work shows that SOC stock changes measured directly in the field can be minimal at the end of both CPs, and that CENTURY
well simulates the SOC dynamics of the stands. The use of such a model, validated at long-term experimental sites, hence represents
an effective tool for estimating future changes in SOC amounts in support of direct measurements when a short period of time,
such as the CP, is considered. 相似文献
179.
Manzo S Rocco A Carotenuto R Picione Fde L Miglietta ML Rametta G Di Francia G 《Environmental science and pollution research international》2011,18(5):756-763
Introduction
Nanomaterials have widespread applications in several industrial sectors. ZnO nanoparticles (NPs) are among the most commonly used metal oxide NPs in personal care products, coating and paints. However, their potential toxicological impact on the environment is largely unexplored. 相似文献180.
Roberto Delogu Andrea Battistone Gabriele Buttinelli Stefano Fiore Stefano Fontana Concetta Amato Karen Cristiano Sabine Gamper Josef Simeoni Rita Frate Laura Pellegrinelli Sandro Binda Licia Veronesi Roberta Zoni Paolo Castiglia Andrea Cossu Maria Triassi Francesca Pennino Cinzia Germinario Viviana Balena Antonella Cicala Pietro Mercurio Lucia Fiore Carlo Pini Paola Stefanelli 《Food and environmental virology》2018,10(4):333-342
Within the initiatives for poliomyelitis eradication by WHO, Italy activated an environmental surveillance (ES) in 2005. ES complements clinical Acute Flaccid Paralysis (AFP) surveillance for possible polio cases, detects poliovirus circulation in environmental sewage, and is used to monitor transmission in communities. In addition to polioviruses, the analyses comprised: (i) the monitoring of the presence of non-polio enteroviruses in sewage samples and (ii) the temporal and geographical distribution of the detected viruses. From 2009 to 2015, 2880 sewage samples were collected from eight cities participating in the surveillance. Overall, 1479 samples resulted positive for enteroviruses. No wild-type polioviruses were found, although four Sabin-like polioviruses were detected. The low degree of mutation found in the genomes of these four isolates suggests that these viruses have had a limited circulation in the population. All non-polio enteroviruses belonged to species B and the most frequent serotype was CV-B5, followed by CV-B4, E-11, E-6, E-7, CV-B3, and CV-B2. Variations in the frequency of different serotypes were also observed in different seasons and/or Italian areas. Environmental surveillance in Italy, as part of the ‘WHO global polio eradication program’, is a powerful tool to augment the polio surveillance and to investigate the silent circulation or the re-emergence of enteroviruses in the population. 相似文献